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Diffusion of dyes in biopolymeric hydrogels
Vyroubal, David ; Mravec, Filip (referee) ; Klučáková, Martina (advisor)
This thesis is focused on diffusion of dyes in biopolymer-based hydrogels. These hydrogels are based on interaction between biopolymer-like electrolytes with oppositely charged surfactants. When polyelectrolytes interact with oppositely charged surfactants, micelle-like nano-containers can be formed. These nano-containers are able of binding hydrophobic compounds. In this study, combination of modified dextran (diethylaminoethyl dextran) with positive charge and oppositely charged sodium dodecylsulphate as surfactant was used for preparation of hydrogels. Next type of hydrogel was based on hyaluronan and positive charged surfactant Septonex (carbethoxypendecinium bromide). As a diffusion probes in hydrogels dyes Nile red and ATTO 488 were used. The diffusion of these dyes from aqeous solutions of NaCl or surfactants into hydrogels was monitored in time. Transport of dyes into structure of hydrogels was characterized by diffusion coefficients and structural parameters of hydrogels.
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Transport properties of dextran-based hydrogel systems
Vyroubal, David ; Kalina, Michal (referee) ; Klučáková, Martina (advisor)
This bachelor thesis is focused on study of transport properties of dextran-based hydrogel systems. The aim of this work is to determine their ability to absorb and release substances. Nile red was used as a probe to examine the transport properties of these systems. All hydrogels were prepared by the so-called wet way. At first stock solutions of dextran and surfactants were prepared and subsequently mixed to form a hydrogel. Nile red was dissolved in the surfactant stock solution and colored gels were formed upon mixing with dextran stock solution. Several types of gels were thus formed which differ in the concentration of the surfactant and nile red used. Supernatants were spectrophotometrically measured at various time intervals. For study of transport properties in multiple surroundings, the original supernatant was replaced after hydrogel preparation with a surfactant solution or physiological saline solution.
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Diffusion of dyes in biopolymeric hydrogels
Vyroubal, David ; Mravec, Filip (referee) ; Klučáková, Martina (advisor)
This thesis is focused on diffusion of dyes in biopolymer-based hydrogels. These hydrogels are based on interaction between biopolymer-like electrolytes with oppositely charged surfactants. When polyelectrolytes interact with oppositely charged surfactants, micelle-like nano-containers can be formed. These nano-containers are able of binding hydrophobic compounds. In this study, combination of modified dextran (diethylaminoethyl dextran) with positive charge and oppositely charged sodium dodecylsulphate as surfactant was used for preparation of hydrogels. Next type of hydrogel was based on hyaluronan and positive charged surfactant Septonex (carbethoxypendecinium bromide). As a diffusion probes in hydrogels dyes Nile red and ATTO 488 were used. The diffusion of these dyes from aqeous solutions of NaCl or surfactants into hydrogels was monitored in time. Transport of dyes into structure of hydrogels was characterized by diffusion coefficients and structural parameters of hydrogels.
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Transport properties of dextran-based hydrogel systems
Vyroubal, David ; Kalina, Michal (referee) ; Klučáková, Martina (advisor)
This bachelor thesis is focused on study of transport properties of dextran-based hydrogel systems. The aim of this work is to determine their ability to absorb and release substances. Nile red was used as a probe to examine the transport properties of these systems. All hydrogels were prepared by the so-called wet way. At first stock solutions of dextran and surfactants were prepared and subsequently mixed to form a hydrogel. Nile red was dissolved in the surfactant stock solution and colored gels were formed upon mixing with dextran stock solution. Several types of gels were thus formed which differ in the concentration of the surfactant and nile red used. Supernatants were spectrophotometrically measured at various time intervals. For study of transport properties in multiple surroundings, the original supernatant was replaced after hydrogel preparation with a surfactant solution or physiological saline solution.
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