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Epithelial uric acid transport proteins and their allelic variants
Vrzáčková, Aneta ; Tlapáková, Tereza (advisor) ; Pavelcová, Kateřina (referee)
In humans, uric acid is the end product of purine metabolism and it is excreted from the body on the one hand by the epithelium lining the tubules of the nephrons, and on the other hand by the intestinal epithelium. This work aims to verify the activity of three candidate transporters from the SLC family (SLC2215, SLC22A18 and SLC47A1) that could transport uric acid in the intestinal epithelium. Preliminary results did not demonstrate that these transporters are involved in uric acid transport in the intestinal epithelium. Furthermore, the work deals with the influence of the W75C allelic variant of the SLC17A1 transporter and the mechanism by which this variant affects the transport of urate in cells. Key words: SLC22A15, SLC22A18, SLC47A1, SLC17A1, transport, epithel, uric acid
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Mechanism of uric acid transport in intestinal epithelium
Vrzáčková, Aneta ; Vávra, Jiří (advisor) ; Zimmermannová, Olga (referee)
Uric acid is main metabolite from purine degradation pathway in humans a higher primates. Serum uric acid level is controlled on one side by absorbtion and on the other side by excretion. This proceses run in kidney nephrone and in intestinal epithelium. This thesis will be summarize the newest knowledges about intestinal epithelium cells transport protein, which excreted or reabsorbed uric acid from lumen of intestine. The thesis will be contain informatin about the transport protein family, protein - substrate interaction, regulation and it's importance in other tissue. Possibilities of regulation the urate handling system in intestinal epithelial, physiological and pathological importance will be contained in this work too. Key words: Uric acid, enterocyte, transport protein, epithelial cell
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