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	A comparison of UV a CAD detection possibilities for selected groups of compounds. Vrkočová, Kateřina ; Nováková, Lucie (advisor) ; Šatínský, Dalibor (referee) This thesis was focused to a comparison of UV and CAD detection for High Liquid Performance Chromathography. The separation of estrogen steroids and phenolic acids was performed on Zorbax SB C18 (50x4,6 mm; 1,8 µm) analytical column. The determination was carried out using two type of detection: ultra - viollet detection (UV) and charged aerosol detection (CAD). The optimized conditions were carried out for determination of estrogen steroids and phenolic acids, especially it was focused on appropriate sensitivity of CAD. The effect of composition and concentration of aqueous part of mobile phase, flow rate of mobile phase and setting of CAD range to its response was monitored. The gradient elution was used for determination of estrogen steroids. The mobile phase was formed by 0,1% acetic acid and acetonitrile. The gradient elution was used for determination of phenolic acids. The mobile phase was formed by 0,5% formic acid and methanol. A part of this thesis was verification of Corona CAD detector applications. CAD enables simultaneous detection of β - estradiol and its five possible impurities at optimized conditions. Relevant results by CAD detection were not obtained in case of the latest eluted cinnamic acid in determination of ten phenolic acids. The method for estrogen steroids was validated... Detailed record
	A comparison of UV a CAD detection possibilities for selected groups of compounds. Vrkočová, Kateřina ; Šatínský, Dalibor (referee) ; Nováková, Lucie (advisor) This thesis was focused to a comparison of UV and CAD detection for High Liquid Performance Chromathography. The separation of estrogen steroids and phenolic acids was performed on Zorbax SB C18 (50x4,6 mm; 1,8 µm) analytical column. The determination was carried out using two type of detection: ultra - viollet detection (UV) and charged aerosol detection (CAD). The optimized conditions were carried out for determination of estrogen steroids and phenolic acids, especially it was focused on appropriate sensitivity of CAD. The effect of composition and concentration of aqueous part of mobile phase, flow rate of mobile phase and setting of CAD range to its response was monitored. The gradient elution was used for determination of estrogen steroids. The mobile phase was formed by 0,1% acetic acid and acetonitrile. The gradient elution was used for determination of phenolic acids. The mobile phase was formed by 0,5% formic acid and methanol. A part of this thesis was verification of Corona CAD detector applications. CAD enables simultaneous detection of β - estradiol and its five possible impurities at optimized conditions. Relevant results by CAD detection were not obtained in case of the latest eluted cinnamic acid in determination of ten phenolic acids. The method for estrogen steroids was validated... Detailed record
	Analysis of Possibilities and Conditions of EU Sources Use for Enterprise Development VRKOČOVÁ, Kateřina Detailed record

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