National Repository of Grey Literature 3 records found  Search took 0.01 seconds. 
Investigation of microstructure and phase transformations in zirconium alloys
Strnad, Adam ; Šmilauerová, Jana (advisor) ; Šíma, Vladimír (referee)
Title: Investigation of microstructure and phase transformations in zirconium alloys Author: Adam Strnad Department: Department of physics of materials Supervisor: RNDr. Jana Šmilauerová, Ph.D., Department of physics of materials Abstract: In this work, we studied microstructure and phase transformations in three α+β zirconium alloys Zr-4Nb-1Cr, Zr-4Nb-2Al and Zr-6Sn-4Nb. We used methods such as light microscopy and scanning electron microscopy to examine the microstructure of α and β phase and its changes due to homogenization annealing. Differential scanning calorimetry and resistometry were employed to investigate the phase transitions occurring in studied alloys. We determined using these complementary methods the temperature dependency of α → β phase transition. As a result, the beta transus temperature was determined for each alloy. By quenching the samples from temperatures below and above the beta transus, we verified the measured value of beta transus temperature. 1
Detection of Ralstonia solanacearum bacteria pathogenic for tomato, pepper and potato by loop-mediated isothermal amplification
STRNAD, Adam
For rapid detection of quarantine bacteria Ralstonia solancearum pathogenic for tomato, potato and papper, the LAMP method was used. Based on the analysis of the fliC gene sequence, a set of LAMP primers was designed to be specific for Ralstonia solanacearum. Subsequently, the method was optimized to detect only Ralstonia solanacearum and to distinguish it from the other phytopathogenic bacteria which infect potato, tomato and pepper. LAMP can detect bacteria from very small amounts of DNA in less than 30 minutes in real time. The method is capable of amplifing DNA at a concentration of 0.00001 ng/?l. However, a higher DNA concentration is more suitable for more specific detection.

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