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Effect of inositol hexaphosphate on adhesion and migration of cell lines SW480 and SW620
Peterková, Alena ; Szotáková, Barbora (advisor) ; Skálová, Lenka (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Alena Ježková Supervisor: Doc. Ing. Barbora Szotáková, Ph.D. Supervisor specialist: RNDr. Veronika Hanušová, Ph.D. Title of diploma thesis: Effect of inositol hexaphosphate on adhesion and migration of cell lines SW480 and SW620 Inositol hexaphosphate (IP6) is molecule, which is usually present in nature, especially in grain and legumes. It has been described several processes, which are influenced in human body by IP6, such as decrease of platelet aggregation, promoting effect on insulin secretion, hypocholesterolemic, as well as anticancer effect. Aim of this work was to study adhesion and migration properties, which are the most important processes in metastasis of cancer cells. For experiments, colorectal adenocarcinoma cell line SW480 and metastatic lymph node cancer cell line SW620, both isolated from identical patient, have been selected. The ability of migration in both cell lines was tested. The influence of IP6 in different concentrations and several time intervals on mRNA (RT-PCR) and protein level (western blot, immunofluorescence) expressions of adhesion and invasion molecules has been compared as well. Considerable ability of migration was found in metastatic cell line...
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Uranium mining, its impact on environment and recultivation
Peterková, Alena ; Malíček, Jiří (advisor) ; Frouz, Jan (referee)
In past, the Czech Republic was one of the most important areas for mineral resources mining. In the period after the Second World War, the country was a very important conqueror of the uranium ore, thanks to a massive production of nuclear energy and nuclear weapons. However, in the 1980s, the interest in uranium declined and in the 1990s almost all of the uranium mines in the country were closed. Rožná was the last place of uranium mining, but it was closed in 2017. Nowadays, there are reclamation works in these mining places. Planning of a reclamation for is not always simply. It is necessary to think about the account of the method of extraction, which was used in the territory, the subsoil, distance from dwellings, underground water, fauna and flora of the surrounding nature etc. The land reclamations are often done in the wrong way and therefore, the extraordinary potential of the landscape is not exploited. People have a strong tendency to interfere this landscape, for example by supplying nutrients for creating of an intensive agricultural landscape. They often do not realize that the mining caused a change of abiotic conditions. The newly created habitats are often inhabited by rare and endangered organisms, living in early succession stages and missing in the surrounding landscape....
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Comparison of the occurrence of invertebrate communities on reclaimed and non-reclaimed heaps after uranium mining
Peterková, Alena ; Frouz, Jan (advisor) ; Reif, Jiří (referee)
This thesis deals with heaps after uranium mining, when not only the physical and chemical properties are affected, but also the biota. During last century in Czech republic uranium mining was important and it significantly affeted the landscape character, for example in Příbram region. Soil heaps after mining were partly reclaimed and partly left for spontaneous succesion. I fit is appropriare to reclaim these areas or leave them for spontaneous succesion or to combine both procedures is a long-standing problem. This paper deals with the comparsion of the occurrence epigeic invertebrate species on two reclaimed, two non-reclaimed spoil heaps and on one control site in their surroundings. Using pitfall traps were collected samples from all these sites during one year. Later they were sorted into classes, Carabidae, Aranea, Diplopoda, Chipoloda and Isopoda were determined into species. Also vegetation samples were made. The result of this work is a comparison of these different sites, founded species and number of individuals of defined groups. It was found, tat the communities od the reclaimed heaps are similar to communities of the control site, while not- reclaimed heaps communities are different. Some endangered, vunerable and near threatened species of Araneae were found. And also two species...
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Effect of inositol hexaphosphate on adhesion and migration of cell lines SW480 and SW620
Peterková, Alena ; Szotáková, Barbora (advisor) ; Skálová, Lenka (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Alena Ježková Supervisor: Doc. Ing. Barbora Szotáková, Ph.D. Supervisor specialist: RNDr. Veronika Hanušová, Ph.D. Title of diploma thesis: Effect of inositol hexaphosphate on adhesion and migration of cell lines SW480 and SW620 Inositol hexaphosphate (IP6) is molecule, which is usually present in nature, especially in grain and legumes. It has been described several processes, which are influenced in human body by IP6, such as decrease of platelet aggregation, promoting effect on insulin secretion, hypocholesterolemic, as well as anticancer effect. Aim of this work was to study adhesion and migration properties, which are the most important processes in metastasis of cancer cells. For experiments, colorectal adenocarcinoma cell line SW480 and metastatic lymph node cancer cell line SW620, both isolated from identical patient, have been selected. The ability of migration in both cell lines was tested. The influence of IP6 in different concentrations and several time intervals on mRNA (RT-PCR) and protein level (western blot, immunofluorescence) expressions of adhesion and invasion molecules has been compared as well. Considerable ability of migration was found in metastatic cell line...
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Uranium mining, its impact on environment and recultivation
Peterková, Alena ; Malíček, Jiří (advisor) ; Frouz, Jan (referee)
In past, the Czech Republic was one of the most important areas for mineral resources mining. In the period after the Second World War, the country was a very important conqueror of the uranium ore, thanks to a massive production of nuclear energy and nuclear weapons. However, in the 1980s, the interest in uranium declined and in the 1990s almost all of the uranium mines in the country were closed. Rožná was the last place of uranium mining, but it was closed in 2017. Nowadays, there are reclamation works in these mining places. Planning of a reclamation for is not always simply. It is necessary to think about the account of the method of extraction, which was used in the territory, the subsoil, distance from dwellings, underground water, fauna and flora of the surrounding nature etc. The land reclamations are often done in the wrong way and therefore, the extraordinary potential of the landscape is not exploited. People have a strong tendency to interfere this landscape, for example by supplying nutrients for creating of an intensive agricultural landscape. They often do not realize that the mining caused a change of abiotic conditions. The newly created habitats are often inhabited by rare and endangered organisms, living in early succession stages and missing in the surrounding landscape....
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