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Effects of triazine based pesticides on fish
MALÝ, Vratislav
Influence of triazine based pesticide (Sencor 70 WG - active substance metribuzine) was assessed via biochemical and hematological blood profile determination of experimental fish. As experimental fish we used common carp (Cyprinus carpio L.) and rainbow trout (Oncorhynchus mykiss ). Before rating the Sencor 70 WG pesticide influence on biochemical and hematological blood profile we accomplished a preliminary test to determine a concentration range and after that a basic acute toxicity test to determine 96hLC50 value. Both fish samples fingerlings were used for performing the tests. After 96hLC50 determination two groups of experimental fish (aged 1-2 years) were under this concentration and one control group without the substance. The test was processed according to the OECD methodology No. 203. Fish dissection was performed after the test. The increased amount of phlegm of water consistency on the body surface was found out. The fish were of dark colour in contrast with the control group. The ventricle was larger and containing transudate. An outstanding vascular profusion of internal organs in the ventricle was found out. Biochemical profile of the rainbow trout (Oncorhynchus mykiss ) experimental group under 96 hour acute operation of pesticide Sencor 70 WG (89,3mg.kg-1) proved significant lowering (p<0,01) of total proteins (TP), triglycerides (TRIG), aspartate aminotransferase (AST), amonnia (NH3), alcaline phosphatase (ALP) and Ca2+ in the blood plasma in contrast with the control group. Hematological profile of the same group proved significant lowering (p<0,05) of the number of erythrocytes (Er), haematocrit (PCV), and significant increase (p<0,05) of erythrocyte haemoglobin (MCH) value in contrast with the control group. Biochemical profile of the common carp (Cyprinus carpio L.) experimental group under 96 hour acute operation of pesticide Sencor 70 WG (250,2 mg.l-1) proved significant lowering (p<0,01) of total proteins (TP), triglycerides (TRIG), lactate dehydrogenase (LDH), Ca2+ and inorganic phosphate (PHOS) and significant increase (p<0,01) of glukose (GLU) and amonnia (NH3) concentration in the blood plasma in contrast with the control group. Hematological profile of the same group proved significant lowering (p<0,01) of the haematocrit (PCV), erytrocyte haemoglobin (MCH), mean erytrocyte volume (MCV) and number of leukocytes (Leuko) in contrast with the control group.
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