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Optimization and validation of UHPSFC-UV methods for quality control of agomelatin and atorvastatin in drug substance and in tablets
Machová, Zdeňka ; Nováková, Lucie (advisor) ; Khalikova, Maria (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Zdeňka Němcová Supervisor: Assoc. Prof. PharmDr. Lucie Nováková, Ph.D. Title of Diploma Thesis: Optimization and validation of UHPSFC-UV methods for quality control of agomelatin and atorvastatin in drug substance and in tablets The aim of this master thesis was to develop two ultra-high performance supercritical fluid chromatography (UHPSFC) methods with PDA detection for determination of agomelatine and atorvastatin and its potential impurities. UHPSFC system Acquity UPC2 with PDA detector was used for measurement. The separation of agomelatine was performed by Torus Diol column (100 × 3.0 mm, 1.7 µm). Gradient elution was performed using CO2 with the mixture of methanol/acetonitrile (1:1) and 31.5 mM (0,1 %) ammonium hydroxide as a mobile phase. Column temperature was set at 40 řC and BPR (back pressure regulator) pressure at 2000 psi. The detection wavelength was set at 225 nm. The method was developed for measurment of API (active pharmaceutical ingredient) and subsequently for tablets. The separation of atorvastatin was performed using Acquity UPLC HSS C18 SB column (100 × 3.0 mm, 1.8 µm). Gradient elution was performed using CO2 with the mixture of methanol/ acetonitrile (2:1), 15 mM...
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Legal aspects of the nursing profession
Máchová, Zdeňka ; Rožnovská, Ludmila (advisor) ; Dvořáčková, Ivana (referee) ; Fejfarová, Radmila (referee)
This title of the thesis is "Legal aspects of the nursing profession". The theoretical part of the thesis describes legal liability, nurse's rights and duties arising from the practise of the nurse profession determined by legislation. This part of the thesis is also focused on the Law about nonmedical care professions. The empirical part of the thesis target the survey of knowledge and adoption of the legislation in practise. At the conclusion there is an evalution of the concrete findings arising from the empirical part.
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Occurrence of mercury in the soil of South Bohemia.
MACHOVÁ, Zdeňka
The work is concerned with mercury appearance in forest and agricultural soils. There is description of process of specimens collection and treatment. The soil specimens were picked up in the selected habitats in South Bohemia. Among studied areas belongs: Lenora near Prachatice, Břehov near České Budějovice, Lužice near Netolice and Velký Chuchelec near Kaplice. There were collected specimens of forest soils, arable land soils, meadows and pastures. Total content of mercury in soils was determined out by the help of atomic absorbtion spectrometric method (CV-AAS) with technique generating cold vapor and gold trap preconcentration. Outcomes were compared with each other in terms of the farming way, locality and vegetation. The data were treated with statistical method ANOVA in programme STATISTICA CZ6. In forest soils there was found the most total content of mercury in the humic horizons in Lenora area on the Chlustov hill - east 0,574 {$\pm$} 0,042 mg.kg-1 dry matter. The least of all of the total content of mercury in forest soils was measured in the second mineral A2 horizon in Břehov area 0,017 {$\pm$} 0,001 mg.kg -1 dry matter. In the agricultural soils there was found the most total quantity of mercury in Lužice area in meadow soil in taking depth into 15 cm 0,088 {$\pm$} 0,007 mg.kg -1 dry matter. On the contrary for at least of total content of mercury was measured out in locality Lužice in arable land in taking depth into 40 cm 0,012 {$\pm$} 0,0001 mg.kg -1 of dry matter. Submission working hypothesis about the influence of land management have the best statistically significantly proved in locality Lužice and Velky Chuchelec. From the results is evident that the way of the use has affect on content of the total content mercury in soil. But there are some other factors which have influence on the mercury content. In the forest soils there was proved statistically relevant effect of sort and age vegetation on the mercury total contant in soils.
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Occurrence of mercury in the soil.
MACHOVÁ, Zdeňka
ABSRTACT Mercury occur in atmosphere, in water also in soil. Work deal with compile literary background research about occurrence mercury in soils and decontamination contaminated soils. Decontamination they may proceed by the help of electrokinetics method, pyrometallurgical processes, biochemical processes and by the help of plants. Work is supplemented about experimental partthat the was compile - time on experimental localities. Main experimental locality finds not far off municipality Velký Chuchelec on Českokrumlovsko. Two next locations find near municipality Břehov on Českobudejovicko and municipality Lužice not far off Netolice on Prachaticko. Locality near Velký Chuchelec is in a number of ways managed meadow. In locality Břehov was take away exhibits arable land and in locality Lusatia was exhibits take away from arable soil, meadowland and wood. Total content mercury in soil was given by the help of atomic absorbtion spectrometry(AAS). Record was compared among single parts locations Velký Chuchelec that the was variously managed. Was processed by the help of statistical methods ANOVA and for content general mercury was quantified correlation with lysimeter waters and with oxidable carbon and organic carbonr. Further was compared contents total mercury among localities mutually and among variants wood, arable land and meadow. Was ascertained that the on experimental locality near Velký Chuchelec was included at least total mercury on flat 1, plot managed skiving three times per annum and amended NPK fertilizers, average content do 0,0265 {$\mu$}g/g. On the contrary high value in those locality was on plot managed mulching in combination with skiving also on flat 1, average value was 0,1171 {$\mu$}g/g. In comparison of others localities was documentary downtrend near kind soil. Differences content mercury in soil among forest soil, meadow and arable soil was conclusive. Downward trend was in sequence: forest soil F+H > meadowland > forest soil A1+A2 > arable land, with next average values in mentioned sequence: 0,1896 {$\mu$}g/g > 0,0924 {$\mu$}g/g > 0,0517{$\mu$}g/g > 0,0401 {$\mu$}g/g.
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