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Analysis of population structure in the seed orchard of wild cherry Prunus avium L.
Krejzková, Anna ; Korecký, Jiří (advisor) ; Čepl, Jaroslav (referee)
The seed orchard of Prunus avium L., Čejkovka, LS LČR Lužná, was established in 2002. Low seed production is characteristic for the orchard. First seed collection took place in 2015, summing up to 35 kilograms. Based on genetic analysis, S locus alleles and microsatellite data were detected. Furthermore, phenology of blooming and fertility of individual ramets was observed. It was detected that from the registered 59 clones, 46 clones were of different genotypes and 29 clones are diferrent in S locus. Approximately 37% ramets in the orchard are genetically identical. In 2015, a total of 45% ramets produced seeds. Based on evidence, the effective number of clones in the orchard is 31, 12 based on SSRs and 10 based on S locus. The number of seed producing clones in 2015 was 20 based on evidence, 8 based on SSRs and only 5 based on S locuses. In order to achieve higher seed production it would be appropriate to partially supplement genetically identical clones with those of different genotypes. An additional option would be utilizing the existing orchard to collect grafts to establish a new, appropriately designed, orchard.
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