|
|
Study of plasma interaction with bacteria for cosmetic applications
Kocianová, Magdaléna ; Krčma, František (referee) ; Kozáková, Zdenka (advisor)
This bachelor thesis is focused on sterilization effects of low temperature plasma on grampositive, anaerobic non-sporulating bacteria Propionibacterium acnes. The plasma sterilization process is fast, effective, non-toxic, environmentally friendly, cost- effective and safe not only for servicing staff, but mainly for the patient. Another advantage of using low temperature plasma is the promotion of cell proliferation and wound healing. The experimental part is conducted to provide an inhibition effect of low-temperature plasma on the Propionibacterium acnes bacterium that causes a purulent inflammation. This bacteria was inoculated in various concentrations on culture soil and treated by plasma at a distance of 1 mm from the agar surface. Two plasma devices were used for plasma generation. One of them was a traditional torch discharge, the second one was based on the surface wave discharge. The microwave discharge was generated in argon at a power of 9 W, gas flow rate of 5 l/min and with water cooling to avoid thermal effects on the treated surface. From the achieved results it can be stated that the low temperature plasma has sterilization effects on a grampositive, anaerobic non-sporulating bacteriu Propionibacterium acnes.
|
|
|
Application of plasma and plasma activated water in cosmetics
Kocianová, Magdaléna ; Tarabová,, Barbora (referee) ; Kozáková, Zdenka (advisor)
This diploma thesis is focused on the study of the inhibitory effects of the cold atmospheric pressure plasma on the grampositive, anaerobic, non-sporulating bacteria Propionibacterium acnes. The treatment was carried out by a direct application of plasma, indirectly (using plasma-treated liquids) and also in their combination. The plasma sterilization process is fast, effective, non-toxic, environmentally friendly, cost-effective and safe not only for the operating staff, but especially for the patient. The experimental part focuses on the study of the direct application of plasma. Two plasma devices for the generation of microwave plasma were used. One of them was a unipolar microwave discharge torch with direct gas supply and the other was a microwave discharge with a surface wave. Discharges were generated in argon with a flow rate of 5 Slm, at a power of 9 W and 12 W. The work also deals with the indirect treatment using plasma-treated liquids. These were prepared using the pinhole discharge and the dielectric barrier discharge. In the last part, combined treatment using plasma-treated liquid and direct plasma treatment was studied. From the achieved results, it can be concluded that the sterilization effects of the plasma and the plasma-treated liquids on the Propionibacterium acnes were confirmed.
|
|
|
Study of plasma interaction with bacteria for cosmetic applications
Kocianová, Magdaléna ; Krčma, František (referee) ; Kozáková, Zdenka (advisor)
This bachelor thesis is focused on sterilization effects of low temperature plasma on grampositive, anaerobic non-sporulating bacteria Propionibacterium acnes. The plasma sterilization process is fast, effective, non-toxic, environmentally friendly, cost- effective and safe not only for servicing staff, but mainly for the patient. Another advantage of using low temperature plasma is the promotion of cell proliferation and wound healing. The experimental part is conducted to provide an inhibition effect of low-temperature plasma on the Propionibacterium acnes bacterium that causes a purulent inflammation. This bacteria was inoculated in various concentrations on culture soil and treated by plasma at a distance of 1 mm from the agar surface. Two plasma devices were used for plasma generation. One of them was a traditional torch discharge, the second one was based on the surface wave discharge. The microwave discharge was generated in argon at a power of 9 W, gas flow rate of 5 l/min and with water cooling to avoid thermal effects on the treated surface. From the achieved results it can be stated that the low temperature plasma has sterilization effects on a grampositive, anaerobic non-sporulating bacteriu Propionibacterium acnes.
|
guest ::
