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Gelation of hydrophobized hyaluronan
Gruberová, Eliška ; Venerová, Tereza (referee) ; Pekař, Miloslav (advisor)
This diploma thesis deals with hyaluronan modified by palmitoyl and its gelation. Gels were created from palmitoyl hyaluronan with molecular weight 216 kDa and degree of substitution 11 % in concentrations 15 and 20 g dm-3 in water and concentrations 10, 15, 20 g dm-3 in NaCl and TSB. Also gel from palmitoyl hyaluronan with molecular weight 35 kDa and degree of substitution 10 % in concentrations 20, 30 g dm-3 in NaCl was created. Gels were investigated concerning medical applications. Gels were rigid and had very good properties, which was confirmed by rheology. The physical properties (pH, water content) of gels and stability were investigated. On the grounds of the MTT test, three methods of cell incorporation were suggested. Gels are nontoxic, biocompatible, and biodegradable with nontoxic degradation products and that is why they are excellent aspirants for use in biomedicine.
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Hydrogels hyaluronan-surfactant: phase diagram
Gruberová, Eliška ; Hurčíková, Andrea (referee) ; Pekař, Miloslav (advisor)
This bachelor’s thesis deals with phase separation of the hyaluronan-cationic surfactant system in a model physiological solution of 0,15 M NaCl resulting in gel formation. The most widely used concentrations of hyaluronan and a wide range of surfactant concentrations was used to form the gel. We have investigated under which conditions the greatest amount of compacted gel was produced. It has been found that phase separation occurs over the entire concentration range. The largest amount of gel was formed at the highest concentration of both hyaluronan and surfactant. The results were plotted in well-arranged graphs. A hydrophobic colorant was used to indicate the presence of micellar formations and the results were depicted in a schematic phase diagram.
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Gelation of hydrophobized hyaluronan
Gruberová, Eliška ; Venerová, Tereza (referee) ; Pekař, Miloslav (advisor)
This diploma thesis deals with hyaluronan modified by palmitoyl and its gelation. Gels were created from palmitoyl hyaluronan with molecular weight 216 kDa and degree of substitution 11 % in concentrations 15 and 20 g dm-3 in water and concentrations 10, 15, 20 g dm-3 in NaCl and TSB. Also gel from palmitoyl hyaluronan with molecular weight 35 kDa and degree of substitution 10 % in concentrations 20, 30 g dm-3 in NaCl was created. Gels were investigated concerning medical applications. Gels were rigid and had very good properties, which was confirmed by rheology. The physical properties (pH, water content) of gels and stability were investigated. On the grounds of the MTT test, three methods of cell incorporation were suggested. Gels are nontoxic, biocompatible, and biodegradable with nontoxic degradation products and that is why they are excellent aspirants for use in biomedicine.
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Hydrogels hyaluronan-surfactant: phase diagram
Gruberová, Eliška ; Hurčíková, Andrea (referee) ; Pekař, Miloslav (advisor)
This bachelor’s thesis deals with phase separation of the hyaluronan-cationic surfactant system in a model physiological solution of 0,15 M NaCl resulting in gel formation. The most widely used concentrations of hyaluronan and a wide range of surfactant concentrations was used to form the gel. We have investigated under which conditions the greatest amount of compacted gel was produced. It has been found that phase separation occurs over the entire concentration range. The largest amount of gel was formed at the highest concentration of both hyaluronan and surfactant. The results were plotted in well-arranged graphs. A hydrophobic colorant was used to indicate the presence of micellar formations and the results were depicted in a schematic phase diagram.
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