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Development of UHPLC-MS/MS method for separation of Amaryllidaceae alkaloids
Burianová, Kristýna ; Nováková, Lucie (advisor) ; Chocholoušová Havlíková, Lucie (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of analytical chemistry Candidate: Kristýna Burianová Supervisor: prof. PharmDr. Lucie Nováková, Ph.D. Consultant: PharmDr. Veronika Pilařová, Ph.D. Title of Doctoral Thesis: Development of UHPLC-MS/MS method for separation of Amaryllidaceae alkaloids In this master thesis, a method for the separation of 30 alkaloids of the Amaryllidaceae family was developed using ultra-high performance liquid chromatography coupled with tandem mass detection (UHPLC-MS/MS). Chromatographic conditions and mass spectrometer parameters were optimized. In the first step of optimization, screening of 10 analytical columns was performed on an ultra-high performance liquid chromatography coupled with photodiode array detection (UHPLC-PDA). Those tested columns were Acquity UPLC BEH C18, Acquity UPLC CSH C18, Acquity UPLC BEH Phenyl, Acquity UPLC CSH Phenyl-hexyl, Arion Plus C18, Acquity UPLC BEH Shield RP18, Ascentis Express RP-Amide, ACE Excel C18-PFP, Kinetex PFP, and Kinetex F5. Acquity UPLC BEH Phenyl, Acquity UPLC CSH Phenyl-hexyl, and Acquity UPLC CSH C18 as they provided the highest separation score for the analyzed isomeric groups of analytes were selected for further optimization. The optimization of MS conditions was performed by...
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Langer's Drama Angels With Us and The Problem of Euthanasia
Buriánová, Kristýna ; Just, Vladimír (advisor) ; Jiřík, Jan (referee)
The purpose of this thesis is to analyze the play of František Langer Angels among us, which he wrote in 1931. Although it was written more than eighty years ago, it still expresses the very topical issues. One of the main themes of the play, which the author addresses is the problem of euthanasia, which has been debated since ancient Greece and Rome and cease debate even today. The paper includes a description of how to treat the theme in the play itself, what opinions on euthanasia prevailed in past centuries, when the play was written and attention is focused even on the present.
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Langer's Drama Angels With Us and The Problem of Euthanasia
Buriánová, Kristýna ; Just, Vladimír (advisor) ; Jiřík, Jan (referee)
The purpose of this thesis is to analyze the play of František Langer Angels among us, which he wrote in 1931. Although it was written more than eighty years ago, it still expresses the very topical issues. One of the main themes of the play, which the author addresses is the problem of euthanasia, which has been debated since ancient Greece and Rome and cease debate even today. The paper includes a description of how to treat the theme in the play itself, what opinions on euthanasia prevailed in past centuries, when the play was written and attention is focused even on the present.
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