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Phospholipids as the basis of biodegradable delivery systems
Burdíková, Jana ; Čeppan, Michal (referee) ; Mravec, Filip (advisor)
This thesis is focused on investigation of phospholipid-hyaluronan system. First, appropriate method for preparation of bulk solution of phospholipid/lipid and suitable fluorescence probe were chosen. Sonification was selected as a method for preparation of bulk solution and pyrene was chosen as a fluorescence probe. From the group of phospholipids lecithin was selected. Next to phospholipid, lipid with no phosphate group (DPTAP) was utilized for comparison, alternatively a mixture of lipid (DPTAP) and phospholipid (DPPC). Instead of hyaluronan another polyelectrolytes (sodium polystyrene sulfonate, sodium alginate) were used too. Measurements were performed in water environment and in phosphate buffer saline (PBS). All investigation was accomplished by fluorescence spectroscopy and dynamic light scattering.
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Preparation and Characterization of Drug Carrier Systems on the Basis of Magnetic Nanoparticles
Chvátalová, Barbora ; Burdíková, Jana (referee) ; Kalina, Michal (advisor)
This bachelor thesis deals with the study of magnetic nanoparticles, optimizing their preparation and stabilization. The nanoparticles with different ratios of Fe2+ and Fe3+ ions were prepared by coprecipitation by variously concentrated sodium hydroxide. Three samples of particles were selected and were further observed. Their stability in Milli-Q water, buffer, polyvinylalcohol, hyaluronic acid and chitosan was studied in function of time using methods of dynamic and electrophoretic light scattering. By UV-VIS spectrometry it was confirmed that the magnetic nanoparticles were actually prepared. Nanoparticles coated with chitosan and hyaluronic acid were then further characterized using FT-IR, XRD and SEM.
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Biocompatible Amphiphilic Compounds and their Interactions with Polymers
Burdíková, Jana ; Sedlařík, Vladimír (referee) ; Bakoš, Dušan (referee) ; Pekař, Miloslav (advisor)
This work is focused on the study of interaction between hyaluronan and high-biocompatible amphiphilic molecules. Using fluorescent probe method, screening of the interaction of cationic lipid 1,2-dipalmitoyl-3-trimethylammonium-propane (DPTAP), mixture of this cationic lipid with zwitterionic lipid, 1,2-dipalmitoyl-sn-glycero-3-phosphochloline (DPPC), with hyaluronan, both native and hydrophobically modified was carried out. Results showed the self-aggregation of DPPC and DPTAP independently on lipids ratio in the mixture and the interaction of DPTAP and DPPC/DPTAP aggregates with hyaluronan at specific ratio of DPTAP and hyaluronan concentration. Physical properties of formed membranes and the influence of cholesterol were also investigated at different DPPC and DPTAP concentration ratio. Last but not least, the non-ionic surfactant-DPPC systems were studied, namely, the size of the formed aggregates, the thermodynamics of solubilisation and the interaction with native hyaluronan.
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Study of interaction between hyaluronan and phospholipid in a presence of biocompatible non-ionic surfactant
Burdíková, Jana ; Klučáková, Martina (referee) ; Mravec, Filip (advisor)
This work is based on investigation of aggregation behavior of sugar surfactants and sn-glycero-dipalmitoylphosphatidylcholin (DPPC) in aqueous environment and on study of the interactions in nonionic surfactant-DPPC and DPPC-nonionic surfactant-hyaluronan systems. Sugar surfactants were used from non-ionic surfactants. The behavior of each systems was studied by fluorescence spectroscopy. Values of critical micellar concentrations (CMC) of sugar surfactants, begining of aggregation of DPPC, DPPC aggregation effect of sugar surfactants and the effect of hyaluronan on the system non-ionic surfactant-DPPC has been investigated. Also CMC of the CPC (cetylpyrimidium chloride), which was used for fluorescence quenching in the determination of aggregate numbers of sugar surfactants, has been determined. Fluorescent probes pyrene, perylene, nile red, acridine orange and hydrophobic dye sudan red were used for measurements.
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Biocompatible Amphiphilic Compounds and their Interactions with Polymers
Burdíková, Jana ; Sedlařík, Vladimír (referee) ; Bakoš, Dušan (referee) ; Pekař, Miloslav (advisor)
This work is focused on the study of interaction between hyaluronan and high-biocompatible amphiphilic molecules. Using fluorescent probe method, screening of the interaction of cationic lipid 1,2-dipalmitoyl-3-trimethylammonium-propane (DPTAP), mixture of this cationic lipid with zwitterionic lipid, 1,2-dipalmitoyl-sn-glycero-3-phosphochloline (DPPC), with hyaluronan, both native and hydrophobically modified was carried out. Results showed the self-aggregation of DPPC and DPTAP independently on lipids ratio in the mixture and the interaction of DPTAP and DPPC/DPTAP aggregates with hyaluronan at specific ratio of DPTAP and hyaluronan concentration. Physical properties of formed membranes and the influence of cholesterol were also investigated at different DPPC and DPTAP concentration ratio. Last but not least, the non-ionic surfactant-DPPC systems were studied, namely, the size of the formed aggregates, the thermodynamics of solubilisation and the interaction with native hyaluronan.
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Phospholipids as the basis of biodegradable delivery systems
Burdíková, Jana ; Čeppan, Michal (referee) ; Mravec, Filip (advisor)
This thesis is focused on investigation of phospholipid-hyaluronan system. First, appropriate method for preparation of bulk solution of phospholipid/lipid and suitable fluorescence probe were chosen. Sonification was selected as a method for preparation of bulk solution and pyrene was chosen as a fluorescence probe. From the group of phospholipids lecithin was selected. Next to phospholipid, lipid with no phosphate group (DPTAP) was utilized for comparison, alternatively a mixture of lipid (DPTAP) and phospholipid (DPPC). Instead of hyaluronan another polyelectrolytes (sodium polystyrene sulfonate, sodium alginate) were used too. Measurements were performed in water environment and in phosphate buffer saline (PBS). All investigation was accomplished by fluorescence spectroscopy and dynamic light scattering.
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Preparation and Characterization of Drug Carrier Systems on the Basis of Magnetic Nanoparticles
Chvátalová, Barbora ; Burdíková, Jana (referee) ; Kalina, Michal (advisor)
This bachelor thesis deals with the study of magnetic nanoparticles, optimizing their preparation and stabilization. The nanoparticles with different ratios of Fe2+ and Fe3+ ions were prepared by coprecipitation by variously concentrated sodium hydroxide. Three samples of particles were selected and were further observed. Their stability in Milli-Q water, buffer, polyvinylalcohol, hyaluronic acid and chitosan was studied in function of time using methods of dynamic and electrophoretic light scattering. By UV-VIS spectrometry it was confirmed that the magnetic nanoparticles were actually prepared. Nanoparticles coated with chitosan and hyaluronic acid were then further characterized using FT-IR, XRD and SEM.
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Study of interaction between hyaluronan and phospholipid in a presence of biocompatible non-ionic surfactant
Burdíková, Jana ; Klučáková, Martina (referee) ; Mravec, Filip (advisor)
This work is based on investigation of aggregation behavior of sugar surfactants and sn-glycero-dipalmitoylphosphatidylcholin (DPPC) in aqueous environment and on study of the interactions in nonionic surfactant-DPPC and DPPC-nonionic surfactant-hyaluronan systems. Sugar surfactants were used from non-ionic surfactants. The behavior of each systems was studied by fluorescence spectroscopy. Values of critical micellar concentrations (CMC) of sugar surfactants, begining of aggregation of DPPC, DPPC aggregation effect of sugar surfactants and the effect of hyaluronan on the system non-ionic surfactant-DPPC has been investigated. Also CMC of the CPC (cetylpyrimidium chloride), which was used for fluorescence quenching in the determination of aggregate numbers of sugar surfactants, has been determined. Fluorescent probes pyrene, perylene, nile red, acridine orange and hydrophobic dye sudan red were used for measurements.
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