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Thick-film sensors for heavy metals detection
Gajdoš, Libor ; Majzlíková, Petra (referee) ; Prášek, Jan (advisor)
This project describes the possibilities of heavy metals detections in solutions using differential pulse voltammetry with tree-electrode electrochemical thick-film sensors. In the theoretical part, the thick-film technology is described as well as thick-film pastes’ types and compositions, followed by introduction to electrochemistry and electro-analytical methods of measurement. This project also describes the three measured heavy metals – cadmium, lead and copper. In the practical part, heavy metals’ ions detection in solutions using carbon working electrode and determination of the detection limit and sensitivity are described and discussed. Finally the evaluation of obtained results and results comparison to hygienic limits are included.
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Electrode system of thick-film electrochemical sensors optimization
Gablech, Imrich ; Adámek, Martin (referee) ; Prášek, Jan (advisor)
This thesis is about optimization of three-electrode electrochemical sensor for detection of substances in solutions fabricated using standard thick film technology. The theoretical part of this thesis describes the thick film technology, introduction to electrochemistry and electro analytical methods for the detection of substances in solutions. The surface optimization of reference and auxiliary electrode of a three-electrode system is solved in the practical part. In conclusions results of the evaluation and description of the ideal three-electrode system are discussed.
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Electrochemical detection of purine bases using thick film Cu2O electrodes
Gabriel, Petr ; Prášek, Jan (referee) ; Pekárková, Jana (advisor)
This work deals with the production process thick films electrodes. It is focused on the Cu2O working electrodes preparation by screen printing and spray coating - methods on the corundum substrate. In this work are described both methods of fabrication and basic electrochemical methods. Prepared Cu2O electrodes were used for electrochemical detection of purine bases (adenine, guanine) using cyclic voltammetry. The effect of pH on the detection of purines was also examines.
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Electrochemical biosensor for glucose detection
Matula, Tomáš ; Majzlíková, Petra (referee) ; Prášek, Jan (advisor)
This thesis pays attention to electrochemical sensors for the detection of glucose. The theoretical part deals with the technology of thick film, their application and firing. It is further described electrochemistry, electrodes used in electrochemistry and analytical methods based on electrochemical reaction. End of theoretical part deals with electrochemical biosensors for detection glucose using special electrodes based on enzyme glucose oxidase. In the experimental part is processed amperometric measure for detection glucose using screenprinted sensors S10 and S8 with immobilized enzyme glucose oxidase. Next was studied effect of plasma, dissolved potassium chloride, storage, ascorbic acid and the reproducibility of the sensors.
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Gas sensors, their construction and testing
Zbožínek, Štěpán ; Prášek, Jan (referee) ; Pytlíček, Zdeněk (advisor)
The presented bachelor´s thesis deals with gas sensors, their construction and methods of testing. First part shortly describes the problems of gas detection. There are introduced common principles, constructions, examples of possible applications and requirements, which should be complied with gas sensors. In more detail, there are described conductivity gas sensors. Main purpose of the work is draft software equipment determined for compiled mixing station. For check correct function of the program was also done several measurements with gas sensors containing active layer SnO2 created for oxygen detection. In conclusion, there is on the base of collected theoretical pieces of knowledge and measured information discussed accuracy of measurement, and limits of usability station.
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Diagnostics of genom conditioned diseases with the use of micro- and nanoparticles
Mondeková, Věra ; Prášek, Jan (referee) ; Provazník, Ivo (advisor)
The bachelor´s thesis discusses possibilities of viral genome´s detection through use of biosensors, more specifically through use of magnetic particles. The introductory part consists of brief characteristic of viruses, mentioned as originator of genom conditioned diseases, followed by chapters related to selected methods of nucleic acid´s extraction and analysis. The main part is dedicated to magnetic particles. The practical part of thesis deals with possibility of use of biosensors in specific viral pathogen´s detection, selection of biocompatible molecules suitable for magnetic particle modification and description of specific DNA sequence isolation procedure through use of magnetic particles.
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Difference pulse voltametry for detection of metallothionein
Postránecká, Tereza ; Prášek, Jan (referee) ; Provazník, Ivo (advisor)
Metallothionein (MT) is a protein with a characteristic amino acid composition which thanks to its thiol groups can bind metal ions and be involved in the homeostasis of these ions in organism. The protein has its significance in carcinogenesis, could be a suitable marker for certain types of tumors. MT has many specific functions in organisms. It can be studied using several techniques. In common use electroanalysis is used as method for determining the concentration of MT. More specifically, this work is focused on differential pulse voltammetry using Brdička reaction.
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Thick-film sensors for electrochemical detection of species in aqueous solutions
Martynek, Daniel ; Adámek, Martin (referee) ; Prášek, Jan (advisor)
This work deals with the theory, principles and characteristics of electrochemical sensors, thick-film technology and electrodes used in electrochemistry. Methods for the detection of substances matters in solutions are discussed here. Main emphasis is given to differential pulse voltammetry and the influence of the method setup parameters to the measurement results. Experimentally, the work deals with the modification of thick-film working electrode with carbon nanotubes and subsequent activation of the electrode by nitric acid to increase the detection limit and the current response to selected heavy metals ions. Finally, the obtained results are compared with special commercial paste and discussed with the data state in literature.
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Thick-film amperometric sensor array
Růžička, Tomáš ; Prášek, Jan (referee) ; Adámek, Martin (advisor)
Supposed work deals with proposal and construction of thick-film amperometric sensor array. The reason of this proposal of sensor array is multiple increase of speed, increase of accuracy and enlargement of possibilities of measuring against current optical system. In theoretic part there is described method of seriography and electrochemical analytic method for determination of chemical elements in the solution. Practical part is composed of proposal of topology of electrochemical sensor electrodes, their comparison according to characteristics and subsequently of the proposal of sensor array. For testing of function of sensor array measuring by method of cyclical voltametry was done with the help of default electrochemical oxidative-reducing pair of potassium ferro-ferricyanide.
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