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Examination of blood for the infectious diseases for the transfusive medicine. Legislature of different countries of the world.
Dohnalová, Eva ; Konečná, Klára (advisor) ; Řeháček, Vít (referee)
Author: Bc. Eva Dohnalová Title: Blood-bank testing for infectious diseases Legislation of different countries of the world Supervisor-specialist: MUDr. Martin Šumpík Supervisor: Mgr. Klára Konečná Diploma thesis Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study specialisation: Specialist in Laboratory Methods A list of most frequently detected infectious agents in blood transfusion service is presented in the first part of this thesis. The history, taxonomy, structure, possible ways of transmission and the most frequently selected diagnostics of detected causative agents are also described in this thesis. In the second part of the thesis, there is described one of the areas of transfusion medicine legislation - blood and blood products testing for infectious diseases. The legislation of some countries of the European Union (the Czech Republic, the Slovak Republic, Poland, Germany, Austria, the United Kingdom of Great Britain and Northern Ireland and France) and the United States of America and their mutual comparison is presented here as well.
Preparation of platelets from apheresis
Slívová, Michaela ; Řeháček, Vít (advisor) ; Jiroušová, Hana (referee)
Bc. Michaela Slívová Preparation of Platelets from Apheresis Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Specialist in Laboratory Methods Background: Aim of the thesis was to evaluate and compare preparation of platelets from apheresis at two departments of the University Hospital Hradec Králové - Transfusion Department (TD) and Department of Clinical Hematology (DCH), using two different types of cell separators - TRIMA Accel version 5.1 and Haemonetics MCS+. Methods: Parameters recorded during platelet collection in the period from August 2010 to January 2011 were used to evaluate characteristics of products and collection procedure properties: collection efficiency, relation between pre donation platelet count and platelet yield, post donation reduction in platelet count, difference between required and acquired platelet yield and leukodepletion. The incidence of adverse reactions of platelet apheresis donors were also recorded. Results: DCH produces more voluminous concentrates with higher platelet yield. The collection efficiency 60 % was determined as satisfactory in both separators. Correlation between pre donation platelet count and platelet yield was positive. Average post donation reduction in platelet count was 27,8 %. There was significant...
Quality control of blood components.
Lebdušková, Jana ; Jiroušová, Hana (referee) ; Řeháček, Vít (advisor)
Transfusion is a treatment procedure that represents the transfer of blood or blood components from the bloodstream into the bloodstream of the donor-recipient patient. Blood transfusion service ensures the quality and safety of therapeutic use of human blood and its components. In order to protect public health and prevent transmission of infectious diseases, shall take all precautionary measures during collection, processing, distribution and use of blood and blood components. Setting high standards of duality and safety of human blood to convince the public that the requirements in all countries of the European Communities. (Directive 2002 / 98/ EC)

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