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WOOX
Kovaříková, Petra ; Weiss, Jan (referee) ; Havliš, Karel (advisor)
The WOOX company is focused on the production of sport goods - mainly clothes. It began to grow and filled up the capacity of its first factory. For the new one, it has chosen the brownfield of the former Jeseník’s textile factory. It is located five minutes from the city center. I approached the owner of this company and because he was just working on the reconstruction, we agreed on cooperation. My intention is to provide the conceptual idea and possible solutions. It should be used as a source of inspiration. Based on my analysis of the plot and the surroundings, my thesis creates a production hall, a design office, dwellings and a multifunctional hall with a bistro. The work is based on SMART principles. It is trying to interconnect the interests of Jeseník’s residents and the company WOOX.
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Analytical and bioanalytical study of selected drugs using UHPLC
Klepalová, Libuše ; Kovaříková, Petra (advisor) ; Kučera, Radim (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of pharmaceutical chemistry and drug analysis Candidate: Libuše Klepalová Supervisor: doc. PharmDr. Petra Kovaříková, Ph.D. Title of the Master's Thesis: Analytical and bioanalytical study of selected drugs using UHPLC Abstract High-performance liquid chromatography coupled with mass spectrometry plays a crucial role in drug analysis from biological materials, mainly due to its sensitivity, specificity and ability to provide structural information on the analytes. Multiple myeloma is a serious hematologic disease, accounting for about 1 % of all oncological diseases. New drugs from the group of proteasome inhibitors namely, carfilzomib and bortezomib represent a great promises for patients with relapsing multiple myeloma or in the case of the resistant to other anticancer drugs. The aim of this work was to develop pilot chromatographic conditions for HPLC-MS analysis of carfilzomib and bortezomib and evaluate different extraction of these drugs from plasma. Separation was carried out on an Ascentis column C18 10 x 3 mm, 3 µm, (Supelco, Germany). Electrospray ionization with ion trap mass analyzer were used in both cases. The mobile phase consisting of water and acetonitrile (20:80) was the most appropriate for carfilzomib. The flow rate of...
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Non-silica based materials in drug analysis III.
Brejchová, Nina ; Kučera, Radim (advisor) ; Kovaříková, Petra (referee)
Non-silica based materials in the drug analysis III. Diploma thesis Nina Brejchová
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Analysis Sample preparation prior to an essay plays a key role in the drug analysis. Example of a widely used sample preparation technique is a Solid Phase Extraction. This method is characterised by high selectivity, which is given by the type of used sorbent. The aim of this thesis is to study the application of a new sorbent in Solid phase extraction which is zirconium oxide modified by graphene. Graphene is an allotropic modification of carbon possessing a specific adsorptive properties. Therefore it can be used besides other areas in chemical analysis. This thesis focuses on the testing on adsorptive capability of this sorbent using a mixture of model basic drugs. The attention was also paid to pH of solution used for conditioning of the sorbent and type of eluents. After the extraction, the samples were analysed by high- performance liquid chromatography, using a gradient elution. The most appropriate extraction environment was aqueous environment. Extraction recoveries obtained with the prepared sorbent were compared to those obtained with silica gel. The highest recoveries were obtained by extraction of...
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Analytical evaluation of selected drugs using UHPLC I
Kalužíková, Barbora ; Kovaříková, Petra (advisor) ; Kučera, Radim (referee)
Barbora Kalužíková Analytical assessment of selected drugs using the UHPLC I Thesis Charles University, Faculty of Pharmacy in Hradec Kralove Study Pharmacy The aim of this work has been to develop an analytic method for the evaluation of sobuzoxane, ICRF-154, and dexrazoxane using UHPLC-UV. Furthermore to verify the linearity of various sobuzoxane concentrations for quantitative evaluation, test of the stability of sobuzoxane in a working solution, and examine sobuzoxane in acid/base stress tests. UHPLC-UV method with gradient elution was developed for the analysis of sobuzoxane, ICRF-154, and dexrazoxane. Analyte separation was achieved using a Zorbax-Aq rapid resolution HT chromatograpjic column (3 mm x 100 mm; 1,8 µm). Methanol and 2 mmol/l ammonium formate acidified to pH 4 with formic acid were used as the mobile phase at following gradient 0 min - 4,5 min 20 % methanol, 4,5 min - 8 min 80 % methanol, 8 min - 11 min 20 % methanol. Analytes were recorded using a UV detector set at a wavelength of 254 nm. Linearity of the method for sobuzoxane was verified in the range (50 µg/ml - 400 µg/ml) using linear regression. The developed method was used to test stability of sobuzoxane in a working solution during analysis and under acid/base stress conditions. The original goal of analyzing one working...
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Analytical evaluation of selected drugs in biological material
Čalkovská, Nikola ; Kovaříková, Petra (advisor) ; Kučera, Radim (referee)
Bisdioxopiperazines have been synthesized for the treatment of tumors with a significant antiproliferative effect. However, they have low bioavailability after oral administration. Consequently, sobuzoxane has been developed. It has been prepared as a prodrug of bisdioxopiperazine ICRF-154 to increase its bioavailability and ease the oral administration for the treatment of lymphomas and leukemias. It is assumed that sobuzoxane is metabolized to an active metabolite of ICRF-154, and then supposedly converted to open analog EDTA-diamide. During preclinical researches of bisdioxopiperazines it has been discovered that they reduce the adverse effects (cardiotoxicity) of anthracyclines. Dexrazoxane has the greatest cardioprotective potential, so it has been used for 20 years as cardioprotective drug. The exact mechanism of antiproliferative and cardioprotective effects has not been fully understood yet. Therefore, the aim of this work is to develop UHPLC-MS / MS method which enables the simultaneous analysis of sobuzoxane and it is anticipated metabolites, which would help in studying bioactivation of sobuzoxane in cardiac cells, in cell medium, and in plasma. There was developed UHPLC-MS/MS method with gradient elution for the analysis of sobuzoxan, ICRF-154 and EDTAm in plasma and cell medium. For...
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Analytical and bioanalytical evaluation of drug candidates from the group of aroylhydrazones
Kresová, Jana ; Kovaříková, Petra (advisor) ; Stariat, Ján (referee)
High-performance liquid chromatography (HPLC) is a progressive analytical method used for qualitative and quantitative drug analysis. This work describes various modifications of the chromatographic method for determining of BSIH (isonicotinic acid [2-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)- benzylidene]-hydrazide) and SIH (salicylaldehyde isonicotinoyl hydrazone) in rabbit plasma. BSIH belongs to prochelators which convert to iron chelator SIH in the presence of hydrogen peroxide. Increased stability in plasma and lower toxicity even during repetitive administration is the advantage of BSIH. Separation of BSIH, SIH and internal standard (o-108) was tested on various stationary phases using different chromatographic conditions. However, none of them provided better results than the initial one. Tha analysis was performed on chromatographic column Zorbax Bonus-RP (150 mm x 3,0 mm, particle size 3,5 µm,) with guard column using identical sorbent at a flow rate of 0,3 ml/min. Phosphate buffer (10 mM monosodium phosphate, pH6, adjusted with sodium hydroxide) : acetonitrile with methanol (60:40) (v/v) with the ratio of 60:40 (v/v)) as the mobile phase. Detector response was registered at 297 nm. Linearity of the method was verified in the concentration range of 10 µM to 100 µM for both BSIH and...
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Studium bioaktivace vybraných proléčiv
Vernerová, Monika ; Kovaříková, Petra (advisor) ; Kastner, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Student: Monika Vernerová Supervisor: Assoc. Prof. PharmDr. Petra Kovaříková, Ph.D. Consultant: Kristiina Huttunen, Ph.D., School of Pharmacy, Faculty of Health Sciences, Kuopio, University of Eastern Finland Title of thesis: Bioactivation study of selected pro-drugs This thesis deals with an enzymatic bioactivation study of the valproic acid's pro- drug. The evaluation was based on the data obtained from the HPLC analysis with appropriate method. The incubation reactions were carried out in a presence of different biological tissues, but also with isolated enzymatic fractions. The half-life of each bioactivation reaction was calculated from the gained results. It was also observed, if the bioconversion of the pro-drug to the parent drug, valproic acid, had been occured. These in vitro results will help to analyse samples from following in vivo experiments and help in designing better pro-drugs in future.
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Non-silica based materials in the drug analysis II.
Rýdlová, Vanda ; Kučera, Radim (advisor) ; Kovaříková, Petra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug analysis Student: Vanda Rýdlová Consultant: PharmDr. Radim Kučera, Ph.D. Title of Thesis: Non-silica materials in drug analysis II. This thesis focuses on non-silica materials and their application in drug analysis. Non-silica materials seem to be a suitable alternative to silica-based materials. This thesis is primarily focused on zirkonia and titania, which are the most intensively studied non-silica materials and their properties, advantages and possibilities of application in separation processes are discussed in many works. For completeness, the part is dedicated to alumina, whose application has been the subject of several studies and articles. But its use is marginal unlike zirkonia and titania.
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Investigation of stability of novel aroylhydrazone iron chelating agents in biological materials II.
Pečinka, Zdeněk ; Kovaříková, Petra (advisor) ; Kučera, Radim (referee)
59 8.Abstract High-performance liquid chromatography (HPLC) is one of the most frequently used separation technique for quantitative and qualitative evaluation of drugs. The present paper deals with optimization of the chromatographic conditions and its application to evaluating the stability of two novel iron chelators 2,6DHAF-INH and AHC-INH in rabbit plasma. The derivates were synthetized so as to increase stability of their hydrazone bond compared to their mother compound of salicylaldehyde isonicotinoyl hydrazone (SIH). The best differentiation of the two evaluated derivates, products of their decomposition and internal standard (SIH) was achieved by means of a chromatographic column with a reverse stationary phase LiChroCART HPLC - cartrige LiChrospher 100 RP - 18e (15µm) with a precolumn. The mobile phase for analysis of 2,6DHAF-INH was selected to be a mix of phosphate buffer (0.01 M aqueous solution NaH2PO4 . 2 H2O s 2 mM EDTA; pH 6.0) and methanol in 47:53(v/v) ratio. Detection at 300 nm wave length, flow 1.0 ml/min, column temperature 25 řC were used. The mobile phase for analysis of AHC-INH was selected to be a mix of phosphate buffer (0.01 M aqueous solution NaH2PO4 . 2 H2O s 2mM EDTA; pH 6.0) and methanol in 50:50(v/v) ratio. Detection at 325 nm wave length, flow 1.0 ml/min, column...
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HPLC evaluation of selected pharmaceuticals VIII
Hynková, Martina ; Mokrý, Milan (advisor) ; Kovaříková, Petra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Student: Martina Hynková Supervisor: RNDr. Milan Mokrý, CSc. Diploma thesis: HPLC evaluation of selected pharmaceuticals VIII RP-HPLC method for simultaneous analysis of Paracetamol, Ascorbic Acid and Phenylephrine in pharmaceutical formulation (powder for oral solution) was developed. In this formulation, the amount of Paracetamol is sixtimes higher then the amount of Phenylephrine. Macherey-Nagel column NUCLEOSIL C18 (150 mm x 4 mm id, 5 µm particle size) was used as a sorbent. Isocratic elution with mobile phase composed of 0,001M monopotassium phosphate puffer and organic modifier methanol (80 : 20, V/V; pH 3,00 - adjusted with 50% phosphoric acid) at a flow rate of 0,7 ml.min-1 and 25 řC in less then 14 minutes. UV detection at the wavelenght 225 nm. Sample volume 10 µl. The influence of variations in mobile phase parameters (pH, content of organic modifier and puffer concentration) was observed. With allopurinol as an IS, calibration was performed to enable quantification of drugs in pharmaceutical formulation.
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