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Influence of hydroxycinnamic acids on methylglyoxal-induced protein glycation
Vejvalková, Hana ; Boušová, Iva (advisor) ; Trnková, Lucie (referee)
Author: Hana Vejvalková Publishing place: Charles University in Prague, Faculty of Pharmacy in Hradec Králové Title: Influence of hydroxycinnamic acids on methylglyoxal-induced protein glycation Supervisor: PharmDr. Iva Boušová, Ph.D. The diploma thesis was aimed at potencial antiglycation effect of antioxidants of natural origin (hydroxycinnamic acids) on the modification of bovine serum albumine and asparate aminotransferase by methylglyoxal as a glycating agent. I monitored the effect of hydroxycinnamic acids on generation of glycation products using fluorescent, absorption, and electrophoretic methods. The influence of hydroxycinnamic acids on the glycation of model proteins was ambiguous. The only derivative with proven antiglycation effect on production of non-specific glycation products and pentosidin measured by fluorescence and spectrophotometry was p-coumaric acid in 100 μM concentration. On the other hand, chlorogenic acid 100 μM displayed strong proglycation effects in all methods used for detection of glycation products. The effect of all tested substances was concentration dependent. Results show that the more substituents given acid contained in its molecule, the stronger proglycation effect this compound exerted.
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Dotační možnosti realizace rozvoje cestovního ruchu v mikroregionu Horňácko
Trnková, Lucie
This thesis evaluates the use of grant funds from various grant schemes for transnational, national, regional and local level in relation to support the development of tourism. The chosen problem area for the evaluation is the micro-region Horňácko. Review of the literature explains the concepts related to the evaluation of strategic documents, programs, projects, contemporary approaches and documentation as well as methods for the evaluation. The thesis also analyzes the main strategic documents related to the development of the monitored area through which the importace of development is confirmed. Especially in the area of tourism there is a potential area specific for such development. There are also included characteristics of the area, history and explanation of the specific municipalities. The usage of financial aids for the development of tourism is evaluated in the analysis of realized projects. Various projects in the field of tourism development in Horňácko are specialy evaluated with objectives of strategic documents and the usage of potential in the area. In the evaluation we find essential persistent problems which are confirmed by the content of the new LAG strategy for the next programming period from 2014 to 2020. There are researched further possibilities of financial aid for new programming period from several sources availible on different levels in the framework of ascertined facts.
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The European Union and phenomenon of migration - Slovenian case
TRNKOVÁ, Lucie
On the beginning of the diploma thesis the student will deal with the issue of migration in general terms, brief explanation of basic terms also from the internatioal law point of view will be presented. Diploma thesis first addresses the phenomenon of migration in general, briefly explains the basic concepts and typology. After this theoretical definition the focus shifts to more practical plane, focusing on selected global migration trends that are currently taking place within the European Union, and approaches to international law on immigration issue, including the European Union's attempt to create a common asylum and migration policy . The final part of the thesis is essentially an analytical study of migration issues on the territory of Slovenia, a relatively prospective member of the European Union with {\clq}qBalkan" history, and his position due to the shaping of their own at first, then Europe's asylum and migration policy.
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