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National Repository of Grey Literature

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	Very low energy STEM for biology Frank, Luděk ; Nebesářová, Jana ; Vancová, Marie ; Paták, Aleš ; Müllerová, Ilona Examination of tissue sections with transmitted electrons has been performed at energies of hundreds and tens of eV with thicknesses of sections of 10 nm or less. This was possible by employing the cathode lens principle working without lowest energy limitations with the help of biasing the sample to a high negative potential. The reflected and transmitted electrons were attracted with the same electric field to earthed detectors situated above and below the sample. Very high image contrasts have been obtained even for samples free of any heavy metal salts for contrast enhancement. Detailed record
	Phytoplasmas occuring in a garden in the South Bohemia Fránová, Jana ; Šimková, Marie ; Přibylová, Jaroslava ; Navrátil, M. ; Nebesářová, Jana Detailed record
	The discovery of potyvirus-like and rhabdovirus-like particles in ultrathin section of Daphne mezereum L Fránová, Jana ; Karešová, R. ; Šimková, Marie ; Navrátil, M. ; Nebesářová, Jana Detailed record
	The first experience with a low voltage electron microscope Nebesářová, Jana The low voltage electron microscope LV EM 5 manufactured by Delong Instruments is described and methods of preparation of biological specimens for the observation in this microscope are briefly evaluated. LV EM 5 is a unique apparatus that could replace in medical and biological research laboratories high volatage transmission electron microscopes. Detailed record
	Observation of biological material in low voltage transmission electron microscope Vancová, Marie ; Nebesářová, Jana Different methods of specimen preparation for low voltage transmission electron microscopy (LVEM) were evaluated. The contrast of the image in LVEM, contrary to high voltage TEM, is strongly influenced by the thickness of the specimen. The resin embedding media used and the process of sectioning employed contributed to image quality and resolution. Detailed record
	Comparison of the cell structure of tissues prepared by different methods for transmission electron microscopy Vancová, Marie ; Nebesářová, Jana The aim of this work is to show how methods which are able to retain antigenicity of tissues can influence the structural appearance of the specimen. We compared the ultrastructure of tissues conventionally embedded in hydrophobic epoxides, with tissues embedded in hydrophilic acrylic resins (polymerized by heat or UV light) and cryo-sections. Detailed record
	The first experience with the specimen preparation for low-voltage transmission electron microscope Nebesářová, Jana ; Vancová, Marie The low-voltage transmission electron microscope, working in the TEM mode, requires ultrathin sections with a thickness 20-30nm. This demand brings problems with a section cohesion caused by insufficient resin infiltration and with a chatter caused by unequal thickness of the section. The contrast of unstained biological specimens in LV TEM is comparable with the contrast obtained in HV TEM of stained specimens. Detailed record
	Electron microscopical study of embryonic envelopes in the tapeworm Proteocephalus torulosus (Cestoda: Proteocephalidea) Bruňanská, Magdaléna ; Nebesářová, Jana ; Scholz, Tomáš The ultrastructure of the embryonic envelopes of the cestode Proteocephalus torulosus (Batsch, 1786), a parasite of cyprinid fish, was examined by transmission electron microscopy. Detailed record
	Ontogeny of fat body in Prorhinotermes simplex (Isoptera: Rhinotemetidae) Weyda, František ; Šobotník, Jan ; Nebesářová, Jana Detailed record
	The importance of fixation procedures for transmission electron microscopy of haemocytes of Pyrrhocoris apterus Slavíčková, K. ; Berger, Josef ; Nebesářová, Jana ; Němec, Václav The importance of fixation procedures for transmission electron microscopy of haemocytes of Pyrrhocoris apterus. Detailed record

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