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Antiproliferative effect of Myrica rubra extracts in cancer cell lines
Štohanslová, Barbora ; Skálová, Lenka (advisor) ; Karlíčková, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Barbora Štohanslová Supervisor: Prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: Antiproliferative effect of Myrica rubra extracts in cancer cell lines Myrica rubra is a woody herb planted in south-eastern Asia, especially in China. Berries, leaves and bark of Myrica rubra plant have been used in Chinese folk medicine for hundreds of years. Recent studies established significant biological effect of Myrica rubra extracts in some of the cancer cell lines. The aim of this study was to test anticancer activity of essential oil from Myrica rubra leaves (MO) to gastrointestinal cell lines (CACO-2 and HCT-8) and study mechanisms of MO effect (production of free radicals and apoptosis activation). Other goal was to compare antiproliferative effect of essential oil's fraction and α-humulene a β-caryophyllene, two most common components found in MO. Proliferation were evaluated by three different methods (NRU, MTT, x-Celligence), quantification of oxidative stress was researched using DCF. Apoptotic marker expression was examined by western blot. Results showed Myrica rubra oil inhibited the proliferation of investigated cell lines; CACO-2 seems to be more sensitive. From acquired...
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Ecotoxicological screening of diazepam drug
Šilhavá, Tereza ; Jahodář, Luděk (advisor) ; Karlíčková, Jana (referee)
54 Abstract (EN) Charles University in Prague, Pharmaceutical Faculty in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Tereza Šilhavá Consultant: RNDr. Jitka Vytlačilová, Ph.D. Title of Thesis: Ecotoxicological screening of diazepam drug Nowadays, information about the influence of medicinal products on the environment is still missing. We have studied the effect of diazepam tablet (5 mg) and the standard on the following organisms: Brachionus calyciflorus (Rotoxkit F Chronic), Thamnocephalus platyurus (Thamnotoxkit FTM ), Desmodesmus subspicatus (algal growth inhibition test). Brachionus calyciflorus was found out as the most sensitive organism also in comparison with already performed tests within the diploma thesis (Rapidtoxkit, Growth inhibition test - Tetrahymena pyriformis, Growth seeds inhibition test - Sinapis alba). Key words: ecotoxicology, diazepam, Brachionus calyciflorus, Thamnocephalus platyurus, Desmodesmus subspicatus
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Copper reducing properties of flavanones
Koblásová, Anna ; Karlíčková, Jana (advisor) ; Jahodář, Luděk (referee)
1 ABSTRACT Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Mgr. Anna Koblásová Consultant: PharmDr. Jana Karlíčková, Ph.D. Title of the Rigorous Thesis: Copper reducing properties of flavanones Copper is an essential trace element, an integral part of many important enzymes involved in a number of vital biological processes. It is necesarry for optimal antioxidant defense of organism. Excess or deficiency of copper cause impaired cellular functions. High concentrations of copper may increase oxidative damage to lipids, proteins, and DNA and contribute to neurodegenerative disorders. Copper deficiency decreases the body's ability to deal with oxidative stress. Free copper ions may catalyze the formation of highly reactive hydroxyl radicals, for that reason they are tight binding to protein carriers. Cuproenzymes are important members of the human antioxidant system. Flavonoids are a large group of polyphenolic compounds with antioxidant and chelating properties. They inhibit lipid peroxidation, formation of reactive oxygen species and chelate redox-active metals. These abilities are influenced by the number, positions, and types of substitutions. Flavonoids have generated an interest because of their broad pharmacological...
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The content of anthocynanidines and other pigments in commercial drugs and food supplements
Karanská, Hana ; Martin, Jan (advisor) ; Karlíčková, Jana (referee)
Name of the work: The content of the anthocyanidines and other pigments in commercial drugs and food supplements Author: Hana Karanská This thesis is focused on the determination of anthocyanins and anthocyanidins in food supplements, fruits and juices. The substances were extracted with methanol using an ultrasonic bath or boiling water bath. Analysis was performed by HPLC. The results of this work show that the content of anthocyanins and anthocyanidins are in the determined samples represented in varying degrees. All the analysed samples showed the content of these substances. Based on information from the manufacturer we can say that the product Ostrovidky plus contained the declared amount of anthocyanins. Manufacturers of remaining specimens does not provide sufficient information about the composition for unambiguous determination of the result, but we can say that the products approximately satisfy the content of substances declared by the manufacturer. The amount of anthocyanins in the fruit meets the values reported in literature. Juices did not indicate the exact amount of anthocyanins.
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Assessment of copper chelation of flavones by bathocuproine method
Michalicová, Alena ; Karlíčková, Jana (advisor) ; Macáková, Kateřina (referee)
Michalicová A., Assessment of copper chelation of flavones by bathocuproine method, Rigorous thesis 2013/2014, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, pp.62. In the human body, copper is the third most widespread trace element. It is responsible for the proper function of various enzymes, which are involved in many important metabolic processes. The maintenance of copper homeostasis is essential for human and its disorders lead to different diseases and pathological changes. Plants produce a large number of metabolites, which are generally divided into primary and secondary. Secondary metabolism, built on primary metabolism, is not necessary for plant life, but offers a wide range of biological effects, used in medicine and pharmacy. Some secondary metabolites from the group of flavones also show chelating activity and are capable of forming chelating complexes with transition elements, such as copper, iron, etc. Thanks to their properties, they could introduce potential therapeutic solutions for copper homeostasis disorders. This thesis deals with the chelating activity of six flavones with the substitution on the ring A - 5-hydroxyflavone, baicalein, baicalin, chrysin, mosloflavone and negletein. Baicalein exhibited the highest chelating activity, while the baicalin...
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Interactions of catechins with copper ions
Řihošková, Petra ; Karlíčková, Jana (advisor) ; Jahodář, Luděk (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical botanic and ecology Candidate: Mgr. Petra Řihošková Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Interactions of catechins with copper ions Keywords: catechins, chelating activity, copper, bathocuproine, hematoxylin, antioxidants Flavonoids are a class of plant polyphenols with significant antioxidant activity, which positively affects human health. Flavonoids are able to chelate metal ions (e.g. iron and copper) which participates in the formation of oxygen free radicals, or they directly react with enzymes whose activity increases oxidative stress. Their effect is influenced by the chemical structure, especially by the number and position of substituents and molecular size. Catechins belong to the group of flavonoids found in various types of food, e.g. in tea, chocolate, fruit, or red wine. Catechins exhibit a broad spectrum of biological effects, therefore they are often studied. Their antioxidant effect is mainly manifested by uptaking or quenching of free radicals and the chelation of iron and copper. Copper is one of the trace elements and is necessary for the proper function of the body. In the human body copper is part of enzymes and affects many metabolic processes. Copper...
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Effects of flavonoids on cardiovascular system
Šramarová, Pavlína ; Karlíčková, Jana (advisor) ; Kašparová, Marie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Pavlína Šramarová Supervisor: PharmDr. Jana Karlíčková, Ph.D Bachelor thesis 2013/2014: Effects of flavonoids on cardiovascular system, pp. 54 Flavonoids are plant secondary metabolities. Flavonoids have a positive influence on human health and show antioxidant activity. They scavenge free radicals and reduce the risk of cardiovascular diseases, inflammatory diseases and cancer. This bachelor thesis explores mechanisms of their action, relationships between structure of flavonoids and antioxidant activity as well as their potential benefit in the prevention and treatment of cardiovascular diseases. In addition, this work provides an overview of the significant effects of certain plants containing flavonoids such as Camellia sinensis, Vitis vinifera, Achillea millefolium, genus Artemisia etc. Key words: flavonoids, the cardiovascular system, effects, antioxidants
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Antiprotozoal activity of alkaloids II.
Kvapilová, Radka ; Vytlačilová, Jitka (advisor) ; Karlíčková, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Author: Radka Kvapilová Supervisor: RNDr. Jitka Vytlačilová, Ph.D. Title of diploma thesis: Antiprotozoal activity of alkaloids II. The development of new antiprotozoal agents for the treatment of infections is very important. Natural substances can be the source of effective drugs. The aim of this study was to evaluate the antiprotozoal activity of these alkaloids - canadine, scoulerine, tetrahydropalmatine and stylopine. The experiment was conducted on typical model organism Tetrahymena thermophila. Percentage inhibition of the organism was determined using the MTT assay. Subsequently median inhibitory concentration IC50 of the test substances was calculated. From our alkaloids stylopine had the greatest antiprotozoal activity. Antiprotozoal activity decreased in the following order stylopine > canadine > scoulerine > tetrahydropalmatine. Key words: Tetrahymena thermophila, antiprotozoal activity, canadine, scoulerine, tetrahydropalmatine, stylopine
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Plants as source of isoflavonoids
Raabová, Karin ; Karlíčková, Jana (advisor) ; Vytlačilová, Jitka (referee)
CHARLES UNIVERSITY IN PRAGUE PHARMACEUTICAL FACULTY IN HRADEC KRÁLOVÉ DEPARTMENT OF PHARMACEUTICAL BOTANY AND ECOLOGY Title of the Bachelor thesis: PLANTS AS SOURCE OF ISOFLAVONOIDS Candidate: Karin Raabová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Bachelor thesis 2014/2015, pp. 65 Isoflavonoids belong to the low molecular weight secondary metabolites of plants produced by many plant families, particularly plants of the family Fabaceae. They are a common part of the human diet (peas, beans, soybeans) and have many biological effects, such as the estrogenic and antioxidant effects, but also can exhibit larvicidal activity. To subsequent establish the presence of these substances in plants are most often used special instrumental (HPLC, MS, DAD) or immunological (ELISA, RIA) methods. This thesis is a literature review, which aimed to gather information about plant sources of isoflavonoids. Among their significant sources of the Kingdom Plantae belong for example Pueraria, Dalbergia, Astragalus, Belamcanda and Gynerium. Keywords: isoflavonoids, plants, biological activity, identification
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