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Contemporary using of medicinal Cannabis in the Czech Republic
Houdková, Tereza ; Jahodář, Luděk (advisor) ; Karlíčková, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Mgr. Tereza Houdková Supervisor: Prof. RNDr. Luděk Jahodář, Csc. Title of doctoral thesis: Contemporary using of medicinal Cannabis in the Czech Republic Cannabis. The plant, which is seen quite controversial. The plant, which has an excellent therapeutic effects, on the other hand, a drug that is frowned upon by many people. With the coming of legalization of therapeutic cannabis in the Czech Republi, the issue gets into the public awareness. The aim of this work is the mapping of legislation and the attitudes of institutions to the official use of cannabis as a drug substance.
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Evaluation of alkaloids using in vivo tests with Artemia salina I.
Balíková, Barbora ; Vytlačilová, Jitka (advisor) ; Karlíčková, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Barbora Balíková Consultant: RNDr. Jitka Vytlačilová, Ph.D. Title of diploma thesis: The evaluation of alkaloids using in vivo tests with Artemia salina I. Alzheimer's disease is the most common cause of dementia and it is one of the lifestyle diseases. There is so far available only limited symptomatic treatment and the occurrence of the lifestyle diseases (and thus Alzheimer's disease) is increasing in recent decades. Therefore it is necessary to find new compounds which will be effective in the treatment of this disease. Toxicological screening - which was our task - is the part of the research of each substance. For the evaluation of the acute toxicity appears as a suitable organism brine shrimp Artemia salina, which was used in the experiment for the purposes of this work. The experiment was conducted in a miniaturized environment. There were tested three substances belonged to the isoquinoline alkaloids: californidine, papaverine and morphine. As the most toxic was evaluated alkaloid californidine, alkaloid papaverine had less toxicity. Morphine appeared essentially as nontoxic with using the same methodology and similar concentrations as in the case of californidine...
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Antiproliferative effect of Myrica rubra extracts in cancer cell lines
Štohanslová, Barbora ; Skálová, Lenka (advisor) ; Karlíčková, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Barbora Štohanslová Supervisor: Prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: Antiproliferative effect of Myrica rubra extracts in cancer cell lines Myrica rubra is a woody herb planted in south-eastern Asia, especially in China. Berries, leaves and bark of Myrica rubra plant have been used in Chinese folk medicine for hundreds of years. Recent studies established significant biological effect of Myrica rubra extracts in some of the cancer cell lines. The aim of this study was to test anticancer activity of essential oil from Myrica rubra leaves (MO) to gastrointestinal cell lines (CACO-2 and HCT-8) and study mechanisms of MO effect (production of free radicals and apoptosis activation). Other goal was to compare antiproliferative effect of essential oil's fraction and α-humulene a β-caryophyllene, two most common components found in MO. Proliferation were evaluated by three different methods (NRU, MTT, x-Celligence), quantification of oxidative stress was researched using DCF. Apoptotic marker expression was examined by western blot. Results showed Myrica rubra oil inhibited the proliferation of investigated cell lines; CACO-2 seems to be more sensitive. From acquired...
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Ecotoxicological screening of diazepam drug
Šilhavá, Tereza ; Jahodář, Luděk (advisor) ; Karlíčková, Jana (referee)
54 Abstract (EN) Charles University in Prague, Pharmaceutical Faculty in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Tereza Šilhavá Consultant: RNDr. Jitka Vytlačilová, Ph.D. Title of Thesis: Ecotoxicological screening of diazepam drug Nowadays, information about the influence of medicinal products on the environment is still missing. We have studied the effect of diazepam tablet (5 mg) and the standard on the following organisms: Brachionus calyciflorus (Rotoxkit F Chronic), Thamnocephalus platyurus (Thamnotoxkit FTM ), Desmodesmus subspicatus (algal growth inhibition test). Brachionus calyciflorus was found out as the most sensitive organism also in comparison with already performed tests within the diploma thesis (Rapidtoxkit, Growth inhibition test - Tetrahymena pyriformis, Growth seeds inhibition test - Sinapis alba). Key words: ecotoxicology, diazepam, Brachionus calyciflorus, Thamnocephalus platyurus, Desmodesmus subspicatus
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Copper reducing properties of flavanones
Koblásová, Anna ; Karlíčková, Jana (advisor) ; Jahodář, Luděk (referee)
1 ABSTRACT Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Mgr. Anna Koblásová Consultant: PharmDr. Jana Karlíčková, Ph.D. Title of the Rigorous Thesis: Copper reducing properties of flavanones Copper is an essential trace element, an integral part of many important enzymes involved in a number of vital biological processes. It is necesarry for optimal antioxidant defense of organism. Excess or deficiency of copper cause impaired cellular functions. High concentrations of copper may increase oxidative damage to lipids, proteins, and DNA and contribute to neurodegenerative disorders. Copper deficiency decreases the body's ability to deal with oxidative stress. Free copper ions may catalyze the formation of highly reactive hydroxyl radicals, for that reason they are tight binding to protein carriers. Cuproenzymes are important members of the human antioxidant system. Flavonoids are a large group of polyphenolic compounds with antioxidant and chelating properties. They inhibit lipid peroxidation, formation of reactive oxygen species and chelate redox-active metals. These abilities are influenced by the number, positions, and types of substitutions. Flavonoids have generated an interest because of their broad pharmacological...
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The content of anthocynanidines and other pigments in commercial drugs and food supplements
Karanská, Hana ; Martin, Jan (advisor) ; Karlíčková, Jana (referee)
Name of the work: The content of the anthocyanidines and other pigments in commercial drugs and food supplements Author: Hana Karanská This thesis is focused on the determination of anthocyanins and anthocyanidins in food supplements, fruits and juices. The substances were extracted with methanol using an ultrasonic bath or boiling water bath. Analysis was performed by HPLC. The results of this work show that the content of anthocyanins and anthocyanidins are in the determined samples represented in varying degrees. All the analysed samples showed the content of these substances. Based on information from the manufacturer we can say that the product Ostrovidky plus contained the declared amount of anthocyanins. Manufacturers of remaining specimens does not provide sufficient information about the composition for unambiguous determination of the result, but we can say that the products approximately satisfy the content of substances declared by the manufacturer. The amount of anthocyanins in the fruit meets the values reported in literature. Juices did not indicate the exact amount of anthocyanins.
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Assessment of copper chelation of flavones by bathocuproine method
Michalicová, Alena ; Karlíčková, Jana (advisor) ; Macáková, Kateřina (referee)
Michalicová A., Assessment of copper chelation of flavones by bathocuproine method, Rigorous thesis 2013/2014, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, pp.62. In the human body, copper is the third most widespread trace element. It is responsible for the proper function of various enzymes, which are involved in many important metabolic processes. The maintenance of copper homeostasis is essential for human and its disorders lead to different diseases and pathological changes. Plants produce a large number of metabolites, which are generally divided into primary and secondary. Secondary metabolism, built on primary metabolism, is not necessary for plant life, but offers a wide range of biological effects, used in medicine and pharmacy. Some secondary metabolites from the group of flavones also show chelating activity and are capable of forming chelating complexes with transition elements, such as copper, iron, etc. Thanks to their properties, they could introduce potential therapeutic solutions for copper homeostasis disorders. This thesis deals with the chelating activity of six flavones with the substitution on the ring A - 5-hydroxyflavone, baicalein, baicalin, chrysin, mosloflavone and negletein. Baicalein exhibited the highest chelating activity, while the baicalin...
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Interactions of catechins with copper ions
Řihošková, Petra ; Karlíčková, Jana (advisor) ; Jahodář, Luděk (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical botanic and ecology Candidate: Mgr. Petra Řihošková Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Interactions of catechins with copper ions Keywords: catechins, chelating activity, copper, bathocuproine, hematoxylin, antioxidants Flavonoids are a class of plant polyphenols with significant antioxidant activity, which positively affects human health. Flavonoids are able to chelate metal ions (e.g. iron and copper) which participates in the formation of oxygen free radicals, or they directly react with enzymes whose activity increases oxidative stress. Their effect is influenced by the chemical structure, especially by the number and position of substituents and molecular size. Catechins belong to the group of flavonoids found in various types of food, e.g. in tea, chocolate, fruit, or red wine. Catechins exhibit a broad spectrum of biological effects, therefore they are often studied. Their antioxidant effect is mainly manifested by uptaking or quenching of free radicals and the chelation of iron and copper. Copper is one of the trace elements and is necessary for the proper function of the body. In the human body copper is part of enzymes and affects many metabolic processes. Copper...
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Effects of flavonoids on cardiovascular system
Šramarová, Pavlína ; Karlíčková, Jana (advisor) ; Kašparová, Marie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Pavlína Šramarová Supervisor: PharmDr. Jana Karlíčková, Ph.D Bachelor thesis 2013/2014: Effects of flavonoids on cardiovascular system, pp. 54 Flavonoids are plant secondary metabolities. Flavonoids have a positive influence on human health and show antioxidant activity. They scavenge free radicals and reduce the risk of cardiovascular diseases, inflammatory diseases and cancer. This bachelor thesis explores mechanisms of their action, relationships between structure of flavonoids and antioxidant activity as well as their potential benefit in the prevention and treatment of cardiovascular diseases. In addition, this work provides an overview of the significant effects of certain plants containing flavonoids such as Camellia sinensis, Vitis vinifera, Achillea millefolium, genus Artemisia etc. Key words: flavonoids, the cardiovascular system, effects, antioxidants
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