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Determination of Potential Drugs in Biological Matrices
Suchardová, Jana ; Kubíček, Vladimír (advisor) ; Lázníčková, Alice (referee)
Charles University of Prague Fakulty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Candidate: Jana Suchardová Consultant: Ing. Vladimír Kubíček, CSc. The aim of this diploma thesis was to find suitable conditions for HPLC determination of VN 014 in biological matrices. Experiments were carried out on four core-shell columns (Ascentis® Express F5, Ascentis® Express C18, Ascentis® Express Phenyl-Hexyl, Ascentis® Express RP-Amide) with different mobile phases. In this thesis two extraction methods were also tested - solid phase extraction and liquid-liquid extraction. The best results were achieved on Ascentis® Express RP-Amide column with the mobile phase composed of 0.03 mol/l formic acid and methanol. LLE method was chosen for the sample preparation. Key words: HPLC, core-shell columns, SPE, LLE, RP-Amide
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Interaction of Tensides and Organic Dyes in Solutions II
Bukatová, Helena ; Kubíček, Vladimír (advisor) ; Lázníčková, Alice (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Candidate: Helena Bukatová Supervisor: Ing. Vladimír Kubíček, CSc. Title of diploma thesis: Interaction of Tensides and Organic Dyes in Solutions II This diploma work is devoted to examination of the aqueous solutions of surfactants and organic dyes in the solution of Britton-Robinson buffer by two spectral methods: UV-VIS absorption spectrophotometry and fluorescence spectroscopy. There are two different dye-surfactant systems investigated: neutral red with sodium lauryl sulfate and phenol red with hexadecyl-trimethyl-ammonium bromide. The possible interactions were observed in the several surfactant concentration levels of the solutions with increased dye concentration. The task of my work was investigation of changes in dye spectra as a consequence of the dye-surfactant interaction and determination of the possible origin of the spectra changes. The results showed that absorption spectra of solutions containing neutral red itself differ sharply from those taken for solutions of neutral red and the tenside. The different character of the spectra is observable from the concentration of the tenside 6∙10-5 mol/l. Two types of dye-tenside associate are probably formed. The associate...
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Interaction of Tensides and Dyes in Solutions I
Belatková, Petra ; Kubíček, Vladimír (advisor) ; Lázníčková, Alice (referee)
This diploma thesis deals with study of interactions between organic dye phenol red and tensides cetyltrimethylamoniumbromide and benzethoniumchloride with using extraction photometry, UV-VIS spectrofotometry and fluorimetry. There were detected changes in spectra of phenol red in presence of both used surfactants, which were ascribed to a formation of premicellar aggregates as a consequence of significant interactions between the dye and the tensides.
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HPLC Analysis of Albendazole and Its Metabolites Using a Core-Shell Column
Králíková, Alena ; Kubíček, Vladimír (advisor) ; Lázníčková, Alice (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Candidate: Alena Králíková Supervisor: Ing. Vladimír Kubíček, CSc. Title of diploma thesis: HPLC Analysis of Albendazole and Its Metabolites Using a Core- Shell Column The HPLC analysis of albendazole and its metabolites using the core-shell column Ascentis® Express RP-Amide is studied in the thesis. A suitable composition of the mobile phase was searched in the first part of the experiment. Two mobile phases of different composition, enabling an excellent separation of albendazole and its metabolites, were found. The linearity of the diode-array detector response in a wide concentration range of the benzimidazoles for both found mobile phases were tested in the second part. Calibration curves for albedazole, albendazole sulphone, and albendazole sulfoxide were constructed using the measured values.
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Study of Interactions between Phenol Red and Cotionic Tensides Using Spectral Methods I.
Belatková, Petra ; Kubíček, Vladimír (advisor) ; Lázníčková, Alice (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Candidate Mgr. Petra Belatková Consultant Ing. Vladimír Kubíček, CSc. Title of Thesis Study of interactions between phenol red and cationic tensides with using spectral methods I This diploma thesis deals with study of interactions between organic dye phenol red and tensides cetyltrimethylamoniumbromide, benzethoniumchloride, cetylpyridiniumchloride and carbethopendeciniumbromide with using fluorimetry with polarized light. In addition, in the case of the last two surfactants the interactions were studied by using extraction photometry, UV-VIS spectrofotometry and classic spectrofluorimetry. The obtained results show that premicellar aggregates, containing both the dye and the tenside species, are formed in the studied solutions due to the significant mutual interaction of both species.
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Study of Fluorescence of Selected Benzimidazoles
Richtrová, Monika ; Kubíček, Vladimír (advisor) ; Lázníčková, Alice (referee)
The influence of used solvents on fluorescence of seven selected benzimidazoles (reduced flubendazole, albendazole, 2-aminobenzimidazole, carbendazim, benomyl, thiabendazole a oxibendazole) is studied in this thesis. It was found, that solvents, used in liquid chromatography as mobile phases, influence the fluorescence of individual benzimidazoles in a very diverse ways. The shape of the fluorescence spectra is very strongly dependent on the structure of a benzimidazole. The obtained results can serve as starting points during a development of chromatographic methods for a benzmidaloles determination with the fluorimetric detection. In the other part of the thesis, a derivatization of hydrolyzed mebendazole with 7- chloro-4-nitrobenzo-2-oxa-1,3-diazole is studied as a way enabling a fluorescence detection of this substance. Unfortunately, the obtained results are not promising.
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