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Phytochemical study of individual plant species of Bergenia genus
Hendrychová, Helena ; Tůmová, Lenka (advisor) ; Nagy, Milan (referee) ; Šmejkal, Karel (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Mgr. Helena Hendrychová Supervisor: doc. PharmDr. Lenka Tůmová, CSc. Title of Doctoral Thesis: Phytochemical study of individual plant species of Bergenia genus Bergenia, native to central Asia, is promising source of medicinal compounds mainly in the system of Chinese and Indian traditional medicine. These species have been used for centuries in therapy of various diseases. They are valued for their ability to dissolve kidney or bladder stones. Bergenia plants are used for therapy of pulmonary diseases and cold. They have demonstrated antioxidant, antibacterial, antiviral, immunostimulant, anti-inflammatory, anticancer, antidiabetic, antitussive, hepatoprotective and diuretic effects. The main aim of this thesis was to determine the content of secondary metabolites (arbutin, total polyphenols, bergenin and anthocyanins) in three taxons of Bergenia genus - Bergenia crassifolia (L.) Fritsch, Bergenia ciliata (Haw.) Sternb. a Bergenia x ornata Stein., to compare their biological activities and to identify the most appropriate kind of Bergenia plants with the greatest potential for use in therapy. Plant hybrid B. x ornata was evaluated on the content of phenolic compounds and biological activity...
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Bioartificial liver in the treatment of experimental acute hepatic failure in a large laboratory animal
Pantoflíček, Tomáš ; Šmejkal, Karel (advisor) ; Král, Vladimír (referee) ; Třeška, Vladislav (referee)
Bioartificial liver in the treatment of experimental acute hepatic failure in a large laboratory animal Aim: To evaluate bioartificial liver (BAL) in the treatment of acute liver failure in the experiment. Methods: 10 minipigs weight 25 - 30 kilograms with experimental acute liver failure (ALF) by surgical devascularization with portocaval by-pass were treated by BAL. The biochemical and hemodynamical parameters of ALF including intracranial pressure (ICP) were measured. The results were compared to control group without BAL treatment. Results: To compare plasma bilirubin there was statistically significant difference between experimental and control groups in 6th and 9th hours to favourite BAL group of minipigs (20,86 vs. 10,60, 22.29 vs. 11,75) and in the 12th hour (37,4 vs. 12,88). The value of ICP in both groups was not significant. Conclusion: The functionality of BAL on our own surgical model of ALF was proven. Initial 85 % viability of fresh isolated porcine hepatocytes and their survival in the time of BAL treatment was reached. Bilirubin plasma concentration has significantly changed in comparison in both groups. ICP values were not changed significantly. Key words: acute liver failure - minipig - bioartificial liver - BAL - Bioreactor - hepatocytes - intracranial pressure
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Caring for patient with colostomy
Kováčová, Lenka ; Šmejkal, Karel (advisor) ; Jandík, Pavel (referee) ; Horáčková, Alexandra (referee)
This BA essay deals with the subject of colostomy patient treatment. Theoretical knowledge, skills, and my practical experiences concerning the care for patients with colostomy are described in two separate chapters. The first chapter describes general knowledge - for instance, the illnesses for which colostomy is determined or the assortment of stomachic aids. The second chapter is more or less concerned about practical experiences -the evaluation of the state of health of colostomy patients or a plan of further care. The empirical part is based upon an examination of skills and knowledge of nurses from Podhorska Hospital, in the area of care for patients with colostomy, using the method of non- standardized questionnaire.
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