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The hydration of hyaluronic acid
Průšová, Alena ; Šimon, Peter (oponent) ; Kučerík, Jiří (vedoucí práce)
Hydration is a crucial factor influencing the secondary structure and consequently the function of molecules present in living systems. Due to mutual affinity of water molecules, they form specific structures which composition and physical properties are affected by the presence of an extraneous molecule. Hyaluronan belongs to the group of biomolecules having large binding and retention capability for water. The aim of this work was to investigate the hydration of different molecular fractions hyaluronan and enumerate the amount of water in individual hydration layers. In the first part, the differential scanning calorimetry was used. In the second part of the work, based on the change in compressibility, the bound water was studied using high resolution ultrasonic spectroscopy.
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Study of Hydrophobic Domains in Humic Acids
Čechlovská, Hana ; Šimon, Peter (oponent) ; Franců, Juraj (oponent) ; Pekař, Miloslav (vedoucí práce)
The nature of hydrophobic domains in humic acids was studied from different points of view. To shed light on the meaning of fluorescent spectra, the measured samples underwent the sequential extraction which partially revealed the role of water-soluble components, free and bond lipids in optical properties of humic acids. The results indicated that the fluorescence peaks traditionally attributed to the superposition of individual chemical structures are rather a result of aggregation properties of humic molecules and hydrophobic effect driving aromatic molecules together forming aggregates apparently large molecular weight. Further, it seems that there is a significant influence of non-fluorophores and non-chromophores on the optical properties of humic acids. Results are consistent with the theory on supramolecular structure of humic acids. Next, the aggregation, conformational behaviour and thermodynamic stability of humic acids were studied by high resolution ultrasonic spectroscopy. It was demonstrated that humic molecules are able to interact and form aggregates at very low concentration (
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The hydration of hyaluronic acid
Průšová, Alena ; Šimon, Peter (oponent) ; Kučerík, Jiří (vedoucí práce)
Hydration is a crucial factor influencing the secondary structure and consequently the function of molecules present in living systems. Due to mutual affinity of water molecules, they form specific structures which composition and physical properties are affected by the presence of an extraneous molecule. Hyaluronan belongs to the group of biomolecules having large binding and retention capability for water. The aim of this work was to investigate the hydration of different molecular fractions hyaluronan and enumerate the amount of water in individual hydration layers. In the first part, the differential scanning calorimetry was used. In the second part of the work, based on the change in compressibility, the bound water was studied using high resolution ultrasonic spectroscopy.
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