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Národní úložiště šedé literatury Nalezeno 2 záznamů.  Hledání trvalo 0.01 vteřin. 
Evaluation of the quality of collagen samples in solid phase using circular dichroism
 Ďubašák, Matej ; Žídek, Jan (oponent) ; Vojtová, Lucy (vedoucí práce)
The diploma thesis deals with the novel qualitative analysis of collagen films by circular dichroism in solid phase. Theoretical part describes collagen and its secondary structure, spectroscopic methods of circular dichroism (CD) and attenuated total reflectance (ATR). The experimental part deals with the preparation of homogeneous and transparent collagen films, the volume, concentration and method of drying optimization. Subsequently, a calibration line with different ratios of collagen and gelatine was measured and evaluated. Obtained linear regression equation was used to determine the content of collagen in the modified samples. The results of evaluated modified samples were compared with ATR spectroscopy measurements. ATR measurements showed higher content of collagen than CD measurements. Samples with the lowest and the highest contents of collagen are same for both methods. It proves that we can compare samples with different collagen content to each other but we cannot determine exact collagen content by connecting these two methods. The optimized method of CD can be used to prepare and measure the exact content of collagen in the samples especially within collagen production to reach the constant quality of collagen at each step of fabrication.
Úplný záznam
Evaluation of the quality of collagen samples in solid phase using circular dichroism
 Ďubašák, Matej ; Žídek, Jan (oponent) ; Vojtová, Lucy (vedoucí práce)
The diploma thesis deals with the novel qualitative analysis of collagen films by circular dichroism in solid phase. Theoretical part describes collagen and its secondary structure, spectroscopic methods of circular dichroism (CD) and attenuated total reflectance (ATR). The experimental part deals with the preparation of homogeneous and transparent collagen films, the volume, concentration and method of drying optimization. Subsequently, a calibration line with different ratios of collagen and gelatine was measured and evaluated. Obtained linear regression equation was used to determine the content of collagen in the modified samples. The results of evaluated modified samples were compared with ATR spectroscopy measurements. ATR measurements showed higher content of collagen than CD measurements. Samples with the lowest and the highest contents of collagen are same for both methods. It proves that we can compare samples with different collagen content to each other but we cannot determine exact collagen content by connecting these two methods. The optimized method of CD can be used to prepare and measure the exact content of collagen in the samples especially within collagen production to reach the constant quality of collagen at each step of fabrication.
Úplný záznam

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