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Národní úložiště šedé literatury Nalezeno 2 záznamů.  Hledání trvalo 0.00 vteřin. 
The cell migration monitoring in a microfluidic system by the "Scratch Wound Healing Assay" method
 Morgaenko, Katsiarina ; Skopalík, Josef (oponent) ; Chmelíková, Larisa (vedoucí práce)
This thesis describes the cell culture methods of mouse embryonic fibroblast (3T3), human umbilical vein endothelial (HUVEC), and Chinese hamster ovary (CHO) cell lines in microfluidic systems simulating capillary. Literature research for microfluidic realizations of Scratch Wound Healing Assay with fibroblasts and endothelial cell lines was performed in this thesis. The principles of confocal and fluorescence microscopy, and relevant image processing techniques used for quantifying cell migration were studied. Experimental setup for microfluidic Scratch Wound Healing Assay with using of trypsin – EDTA for creation of cell delusion, and confocal microscope Leica TCS SP8 X for subsequent image acquisition was proposed and tested. An appropriate algorithm for cell migration analysis was written in Matlab computing environment. Experiment’s results are discussed in the conclusion of this thesis.
Úplný záznam
The cell migration monitoring in a microfluidic system by the "Scratch Wound Healing Assay" method
 Morgaenko, Katsiarina ; Skopalík, Josef (oponent) ; Chmelíková, Larisa (vedoucí práce)
This thesis describes the cell culture methods of mouse embryonic fibroblast (3T3), human umbilical vein endothelial (HUVEC), and Chinese hamster ovary (CHO) cell lines in microfluidic systems simulating capillary. Literature research for microfluidic realizations of Scratch Wound Healing Assay with fibroblasts and endothelial cell lines was performed in this thesis. The principles of confocal and fluorescence microscopy, and relevant image processing techniques used for quantifying cell migration were studied. Experimental setup for microfluidic Scratch Wound Healing Assay with using of trypsin – EDTA for creation of cell delusion, and confocal microscope Leica TCS SP8 X for subsequent image acquisition was proposed and tested. An appropriate algorithm for cell migration analysis was written in Matlab computing environment. Experiment’s results are discussed in the conclusion of this thesis.
Úplný záznam

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