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Determination of basic chemical characteristics of blackberry pulp
Pilátová, Anna ; Diviš, Pavel (referee) ; Vespalcová, Milena (advisor)
This bachelor thesis deals with determination of selected chemical characteristics of juice, pulp and extract prepared from blackberry of two species, from The Czech Republic and Ukraine. The theoretical part contains the botanical characteristics of blackberry, the content of active substances and possibilities of use of blackberries in the food industry. The following chapters focus on the description of phenolic substances and the determination of pH and titration acidity of fruit juices by manual methods. In the experimental part, two extraction solvents were first compared for extraction of anthocyanins from blackberry moldings. Furthermore, the selected chemical and physical properties of extracts, juice and pulp from blackberries were determined. The analysis was carried out for blackberries from the Czech Republic and Ukraine. A 1 : 1 mixture of distilled water and ethanol was a better extraction solvent for the content of anthocyanins. After 14 hours of maceration 150.4 mg of anthocyanins was obtained from 100,0 g of blackberry moldings Extract from blackberry moldings has been determined to have a much higher concentration of anthocyanins than juice and pulp. The extract of Ukrainian blackberries contained 197.4 mg CGEdm-3 and extract of Czech blackberries 150.4 mg CGEdm-3. Also phenolic substances had the highest concentration in extracts. Ukrainian blackberries contained 3501.9 mg GAEdm-3 and Czech blackberries contained 3256.1 mg GAEdm-3.
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Bioactive substances contained in elderberry pomace
Šimšová, Veronika ; Diviš, Pavel (referee) ; Vespalcová, Milena (advisor)
This bachelor thesis deals with the determination of selected chemical characteristics in the elderberry juice, compote and in the extracts of elderberry marc. The theoretical part describes the botanical characteristics of elderberry (Sambucus nigra L.) including its occurrence, the possibility of utilization of individual parts of the plant in the food industry and in the healing therapies and chemical composition of the berries. The biggest attention is given to the bioactive compounds contained in these fruits. The second chapter deals with the dry matter and the methods of its determination. The last chapter of the theoretical part describes vitamin C, its importance for the human organism and manual methods for determination of vitamin C. Among the selected chemical characteristics, set out in the experimental part, was the determination of reducing sugars by gravimetric method and Berthrand method, titratable acids, total anthocyanins by pH-differential method, total phenolic compounds by the Folin-Ciocalteu method and determination of the dry matter by drying and refractometric method. Analyzes have proven that extracts made of elderberry marc under suitable conditions have, in most cases, chemical characteristics comparable to the elderberry juice and compote. However, extracts are much better source of anthocyanic and phenolic compounds than juice and compote.
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Bioactive substances contained in elderberry pomace
Šimšová, Veronika ; Diviš, Pavel (referee) ; Vespalcová, Milena (advisor)
This bachelor thesis deals with the determination of selected chemical characteristics in the elderberry juice, compote and in the extracts of elderberry marc. The theoretical part describes the botanical characteristics of elderberry (Sambucus nigra L.) including its occurrence, the possibility of utilization of individual parts of the plant in the food industry and in the healing therapies and chemical composition of the berries. The biggest attention is given to the bioactive compounds contained in these fruits. The second chapter deals with the dry matter and the methods of its determination. The last chapter of the theoretical part describes vitamin C, its importance for the human organism and manual methods for determination of vitamin C. Among the selected chemical characteristics, set out in the experimental part, was the determination of reducing sugars by gravimetric method and Berthrand method, titratable acids, total anthocyanins by pH-differential method, total phenolic compounds by the Folin-Ciocalteu method and determination of the dry matter by drying and refractometric method. Analyzes have proven that extracts made of elderberry marc under suitable conditions have, in most cases, chemical characteristics comparable to the elderberry juice and compote. However, extracts are much better source of anthocyanic and phenolic compounds than juice and compote.
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Determination of basic chemical characteristics of blackberry pulp
Pilátová, Anna ; Diviš, Pavel (referee) ; Vespalcová, Milena (advisor)
This bachelor thesis deals with determination of selected chemical characteristics of juice, pulp and extract prepared from blackberry of two species, from The Czech Republic and Ukraine. The theoretical part contains the botanical characteristics of blackberry, the content of active substances and possibilities of use of blackberries in the food industry. The following chapters focus on the description of phenolic substances and the determination of pH and titration acidity of fruit juices by manual methods. In the experimental part, two extraction solvents were first compared for extraction of anthocyanins from blackberry moldings. Furthermore, the selected chemical and physical properties of extracts, juice and pulp from blackberries were determined. The analysis was carried out for blackberries from the Czech Republic and Ukraine. A 1 : 1 mixture of distilled water and ethanol was a better extraction solvent for the content of anthocyanins. After 14 hours of maceration 150.4 mg of anthocyanins was obtained from 100,0 g of blackberry moldings Extract from blackberry moldings has been determined to have a much higher concentration of anthocyanins than juice and pulp. The extract of Ukrainian blackberries contained 197.4 mg CGEdm-3 and extract of Czech blackberries 150.4 mg CGEdm-3. Also phenolic substances had the highest concentration in extracts. Ukrainian blackberries contained 3501.9 mg GAEdm-3 and Czech blackberries contained 3256.1 mg GAEdm-3.
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