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Functionalization of gold nanoparticles for imaging
Jakubechová, Jana ; Pekárková, Jana (referee) ; Drbohlavová, Jana (advisor)
The diploma thesis deals with synthesis of gold nanoparticles and their surface functionalization suitable for in vitro imaging. In this view there are requirements for optical properties such as stability, monodispersity and no presence of cytotocxicity. In order to fulfill these demands the synthesis by Turkevich method with surface modification by glutathione and polytethylenglycol was performed. Analytical methods such as DLS, SEM and Zeta potential measurement were utilized to characterize the physical and chemical properties of synthesized gold nanoparticles. Finally, MTT assay was performed to evaluate toxicity of gold nanoparticles using HEK 293 cell line.
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Comparison of the imaging capabilities of the transmission and scanning electron microscope
Maňka, Tadeáš ; Šamořil, Tomáš (referee) ; Zlámal, Jakub (advisor)
The aim of this work is to compare the imaging capabilities of the transmission and scanning electron microscope. The theoretical part describes the construction and the principle of each type of microscope. There is a list of the signal detectors, and the principle of imaging is described. The samples and their preparation are described. The practical part is aimed to imaging possibilities of both microscopes. A standard calibration samples were used for comparison. The advantages and disadvantages of both microscopes were discussed.
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Functionalization of gold nanoparticles for imaging
Jakubechová, Jana ; Pekárková, Jana (referee) ; Drbohlavová, Jana (advisor)
The diploma thesis deals with synthesis of gold nanoparticles and their surface functionalization suitable for in vitro imaging. In this view there are requirements for optical properties such as stability, monodispersity and no presence of cytotocxicity. In order to fulfill these demands the synthesis by Turkevich method with surface modification by glutathione and polytethylenglycol was performed. Analytical methods such as DLS, SEM and Zeta potential measurement were utilized to characterize the physical and chemical properties of synthesized gold nanoparticles. Finally, MTT assay was performed to evaluate toxicity of gold nanoparticles using HEK 293 cell line.
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Comparison of the imaging capabilities of the transmission and scanning electron microscope
Maňka, Tadeáš ; Šamořil, Tomáš (referee) ; Zlámal, Jakub (advisor)
The aim of this work is to compare the imaging capabilities of the transmission and scanning electron microscope. The theoretical part describes the construction and the principle of each type of microscope. There is a list of the signal detectors, and the principle of imaging is described. The samples and their preparation are described. The practical part is aimed to imaging possibilities of both microscopes. A standard calibration samples were used for comparison. The advantages and disadvantages of both microscopes were discussed.
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