Home > Search Results: Zobalová, Eliška
Search:
Search Tips :: Advanced Search
Search collections:
Sort by: Display results: Output format:
National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
The role of Fbxo38 ubiquitin ligase in mouse spermatogenesis
 Zobalová, Eliška ; Čermák, Lukáš (advisor) ; Stopka, Pavel (referee)
Cullin-dependent ubiquitin ligases are responsible for the regulation of most cellular processes. Despite their mutated forms being the cause of many human diseases, their physiological roles are not sufficiently described. In the presented results, we focused on the physiological role of ubiquitin ligase SCFFBXO38 (SKP1-CULLIN1-FBXO38), whose mutated forms are responsible for the progression of distal neuropathy. Preparation of mouse model deficient in FBXO38 revealed that homozygous pups were born in a lower than expected ratio. Animals were growth-retarded, both at the level of the whole organism and individual organs, especially the liver and testes. Males with a deletion in the Fbxo38 gene had significantly lower reproductive capacity, which was associated with lower production of mature sperm and pathological changes in the structure of seminiferous tubules. We found that the FBXO38 protein is functionally expressed in Sertoli cells responsible for regulating spermatogenesis and seminiferous tubules integrity. Detailed analysis of spermatogenic populations revealed a defect at the level of spermatocyte differentiation. The dynamics of this differentiation depend on the hematotesticular barrier functional integrity formed by the intercellular junctions of Sertoli cells. We confirmed that the...
Detailed record
Pseudotyping in baculoviruses
 Zobalová, Eliška ; Španielová, Hana (advisor) ; Fraiberk, Martin (referee)
The baculoviruses are a group of enveloped DNA viruses that infect the larval stage of arthropods, mainly insects. They are widely used in biotechnology and well known for their utility as biological pesticides and gene expression vectors for the production of proteins in insect cells and larvae. However, they are also able to enter in mammalian cells and deliver-genes for expression under the control of mammalian cell-active promoters. Recombination or pseudotyping can result in formation of baculoviruses that provide a higher transduction frequency or are able to recognize specific mammalian cells. This bachelor thesis describes the phenomenon of viral pseudotyping and summarizes published information about the use of pseudotyped baculoviruses for gene therapy and vaccination.
Detailed record

See also: similar author names
1 ZOBALOVÁ, Eva
Interested in being notified about new results for this query?
Subscribe to the RSS feed.
Digital Repository :: Search :: Submit :: Personalize :: Help
Powered by Invenio v1.1.2
Maintained by admin.invenio@techlib.cz
This site is also available in the following languages:
Česky  English