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Neurotropic and antioxidative activity in vitro of monocotyledonous alkaloidal plants.
Zavadil, Stanislav ; Cahlíková, Lucie (advisor) ; Valterová, Irena (referee)
Zavadil S.: Neurotropic and antioxidative activity in vitro of monocotyledonous alkaloidal plants. Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2011, 74 p. The aim of the diploma thesis was to process screening of total and alkaloidal extracts from four selected plants of the Amaryllidaceae family (Hymenocallis sp., Chlidanthus fragrans, Nerine bowdenii, Zephyranthes grandiflora) and test their anticholinesterase activities to human blood acetylcholinesterase (HuAChE) and to human plasma butyrylcholinesterase (HuBuChE). For the purpose of preliminary determination of substances that could be responsible for the biological activity of these extracts, GC/MS analysis of alkaloidal extracts was carried out. All of these plants were analyzed by GC/MS for the first time. During the processing of GC/MS data we identified alkaloids, which weren't in these plants previously described. The alkaloidal extract of Chlidanthus fragrans showed the strongest inhibition of HuAChE (IC50 = 20.1 ± 2.9 µM) and the alkaloidal extract from Nerine bowdenii was the most potent inhibitor of HuBuChE (IC50 = 4.8 ± 1.1 µM). All alkaloidal extracts didn't show an interesting antioxidant activity. The alkaloid belladine was...
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Criminal and criminological aspects of ransomware spreading
Zavadil, Stanislav ; Gřivna, Tomáš (advisor) ; Bohuslav, Lukáš (referee)
Criminal and criminological aspects of ransomware spreading Abstract This diploma thesis deals with issues of ransomware spreading and examines certain criminal and criminological aspects of this cybercrime phenomenon. Ransomware is malware that encrypts, blocks or prevents access to the computer system or data in a computer system. In connection to this, it demands monetary or other ransom. This diploma thesis firstly describes ransomware from the point of view of its function and technical aspects, including its history, categorization of its variations and description of several notable infection examples, namely WannaCry, Petya, DoubleLocker and Vir Policie. Following section describes possible criminal qualifications according to Czech substantive criminal law, including the consideration of specifics of different ransomware variations and potential development of this criminal aktivity. The final part focuses on criminological aspects of ransomware spreading. It beggins with a description of the crime status and dynamics, including further details about latency and trends. Then follows the description of perpetrator and victim in view of certain criminological theories. Finally, criminological part comprises a chapter about crime control and prevention, which includes practical parts that aim to help...
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Neurotropic and antioxidative activity in vitro of monocotyledonous alkaloidal plants.
Zavadil, Stanislav ; Cahlíková, Lucie (advisor) ; Valterová, Irena (referee)
Zavadil S.: Neurotropic and antioxidative activity in vitro of monocotyledonous alkaloidal plants. Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2011, 74 p. The aim of the diploma thesis was to process screening of total and alkaloidal extracts from four selected plants of the Amaryllidaceae family (Hymenocallis sp., Chlidanthus fragrans, Nerine bowdenii, Zephyranthes grandiflora) and test their anticholinesterase activities to human blood acetylcholinesterase (HuAChE) and to human plasma butyrylcholinesterase (HuBuChE). For the purpose of preliminary determination of substances that could be responsible for the biological activity of these extracts, GC/MS analysis of alkaloidal extracts was carried out. All of these plants were analyzed by GC/MS for the first time. During the processing of GC/MS data we identified alkaloids, which weren't in these plants previously described. The alkaloidal extract of Chlidanthus fragrans showed the strongest inhibition of HuAChE (IC50 = 20.1 ± 2.9 µM) and the alkaloidal extract from Nerine bowdenii was the most potent inhibitor of HuBuChE (IC50 = 4.8 ± 1.1 µM). All alkaloidal extracts didn't show an interesting antioxidant activity. The alkaloid belladine was...
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