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Development and testing of capillary columns based on ion exchanger
Vojta, Jiří ; Coufal, Pavel (advisor) ; Bosáková, Zuzana (referee)
In this work, capillary monolithic columns based on styrene, divinylbenzene and methacrylic acid were prepared by free radical polymerization inside fused sillica capillaries of 320 µm inner diameter. The columns were prepared by very simple process from polymerization mixtures with variable amount of methacrylic acid. Effect of electrostatic interactions between methacrylic acid, as weak cation-exchanger, and analytes on their retention was showed. Based on chromatographic measurements, effect of amount of methacrylic acid in polymerization mixtures on the morphological properties of monoliths and on the retention of small organic amines and benzene was discussed.
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Use of liquid chromatography in pharmaceutical analysis and preparation of monolithic stationary phases for thin-layer chromatography
Vojta, Jiří ; Coufal, Pavel (advisor) ; Tůma, Petr (referee) ; Jelínek, Ivan (referee)
(EN) In the first part of this work, analytical methods for determination of impurities of active pharmaceutical ingredients (API) in combined pharmaceutical dosage forms were developed and validated. Development of the methods covered both the optimization of sample preparation procedure and chromatographic conditions. The methods were validated according to International Conference on Harmonization guideline and both of them were confirmed to be able to analyze stability samples. Impurities in paracetamol, codeine phosphate hemihydrate and pitophenone hydrochloride in the presence of fourth API fenpiverinium bromide were separated by using ion-pair reversed phase chromatography with gradient elution. Symmetry C18, 250 x 4,6 mm, 5 µm heated to 35 řC was used as a separation column. A diode array detector was used. The detection wavelengths were set as follows: 220 nm for paracetamol impurity K, 245 nm for paracetamol and its other impurities and 285 nm for codeine, pitophenone and their impurities. Impurities in valsartan, amlodipine besylate and hydrochlorothiazide were separated by reversed phase UHPLC method with gradient elution. Chromatographic column Zorbax Eclipse C8 RRHD, 100 x 3,0 mm, 1,8 µm heated to 30 řC and spectrophotometric detection were used. The detection wavelengths were set as...
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Use of liquid chromatography in pharmaceutical analysis and preparation of monolithic stationary phases for thin-layer chromatography
Vojta, Jiří
(EN) In the first part of this work, analytical methods for determination of impurities of active pharmaceutical ingredients (API) in combined pharmaceutical dosage forms were developed and validated. Development of the methods covered both the optimization of sample preparation procedure and chromatographic conditions. The methods were validated according to International Conference on Harmonization guideline and both of them were confirmed to be able to analyze stability samples. Impurities in paracetamol, codeine phosphate hemihydrate and pitophenone hydrochloride in the presence of fourth API fenpiverinium bromide were separated by using ion-pair reversed phase chromatography with gradient elution. Symmetry C18, 250 x 4,6 mm, 5 µm heated to 35 řC was used as a separation column. A diode array detector was used. The detection wavelengths were set as follows: 220 nm for paracetamol impurity K, 245 nm for paracetamol and its other impurities and 285 nm for codeine, pitophenone and their impurities. Impurities in valsartan, amlodipine besylate and hydrochlorothiazide were separated by reversed phase UHPLC method with gradient elution. Chromatographic column Zorbax Eclipse C8 RRHD, 100 x 3,0 mm, 1,8 µm heated to 30 řC and spectrophotometric detection were used. The detection wavelengths were set as...
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Hlučnost výrobních strojů ve vybraném podniku
VOJTA, Jiří
The diploma thesis deals with noise measurement. The theoretical part contains the knowledge gained by studying literature. I have described acoustics, sound and noise, noise and health and legislative contexts as well as noise reduction methods. The practical part contains information about the company and individual machinery, the results of measurements and their evaluation according to valid legislation. I have suggested appropriate measures to reduce the noise burden on operators.
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Use of liquid chromatography in pharmaceutical analysis and preparation of monolithic stationary phases for thin-layer chromatography
Vojta, Jiří ; Coufal, Pavel (advisor) ; Tůma, Petr (referee) ; Jelínek, Ivan (referee)
(EN) In the first part of this work, analytical methods for determination of impurities of active pharmaceutical ingredients (API) in combined pharmaceutical dosage forms were developed and validated. Development of the methods covered both the optimization of sample preparation procedure and chromatographic conditions. The methods were validated according to International Conference on Harmonization guideline and both of them were confirmed to be able to analyze stability samples. Impurities in paracetamol, codeine phosphate hemihydrate and pitophenone hydrochloride in the presence of fourth API fenpiverinium bromide were separated by using ion-pair reversed phase chromatography with gradient elution. Symmetry C18, 250 x 4,6 mm, 5 µm heated to 35 řC was used as a separation column. A diode array detector was used. The detection wavelengths were set as follows: 220 nm for paracetamol impurity K, 245 nm for paracetamol and its other impurities and 285 nm for codeine, pitophenone and their impurities. Impurities in valsartan, amlodipine besylate and hydrochlorothiazide were separated by reversed phase UHPLC method with gradient elution. Chromatographic column Zorbax Eclipse C8 RRHD, 100 x 3,0 mm, 1,8 µm heated to 30 řC and spectrophotometric detection were used. The detection wavelengths were set as...
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Use of liquid chromatography in pharmaceutical analysis and preparation of monolithic stationary phases for thin-layer chromatography
Vojta, Jiří
(EN) In the first part of this work, analytical methods for determination of impurities of active pharmaceutical ingredients (API) in combined pharmaceutical dosage forms were developed and validated. Development of the methods covered both the optimization of sample preparation procedure and chromatographic conditions. The methods were validated according to International Conference on Harmonization guideline and both of them were confirmed to be able to analyze stability samples. Impurities in paracetamol, codeine phosphate hemihydrate and pitophenone hydrochloride in the presence of fourth API fenpiverinium bromide were separated by using ion-pair reversed phase chromatography with gradient elution. Symmetry C18, 250 x 4,6 mm, 5 µm heated to 35 řC was used as a separation column. A diode array detector was used. The detection wavelengths were set as follows: 220 nm for paracetamol impurity K, 245 nm for paracetamol and its other impurities and 285 nm for codeine, pitophenone and their impurities. Impurities in valsartan, amlodipine besylate and hydrochlorothiazide were separated by reversed phase UHPLC method with gradient elution. Chromatographic column Zorbax Eclipse C8 RRHD, 100 x 3,0 mm, 1,8 µm heated to 30 řC and spectrophotometric detection were used. The detection wavelengths were set as...
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Development and testing of capillary columns based on ion exchanger
Vojta, Jiří ; Coufal, Pavel (advisor) ; Bosáková, Zuzana (referee)
In this work, capillary monolithic columns based on styrene, divinylbenzene and methacrylic acid were prepared by free radical polymerization inside fused sillica capillaries of 320 µm inner diameter. The columns were prepared by very simple process from polymerization mixtures with variable amount of methacrylic acid. Effect of electrostatic interactions between methacrylic acid, as weak cation-exchanger, and analytes on their retention was showed. Based on chromatographic measurements, effect of amount of methacrylic acid in polymerization mixtures on the morphological properties of monoliths and on the retention of small organic amines and benzene was discussed.
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The role of a radiation monitoring and a dosimetric provision in the first phase of a local radiation emergency
VOJTA, Jiří
In the last decades, the sources of ionizing radiation have been used more and in various branches of medicine, industries, agriculture and research. Like each human activity, the usage of the sources of ionizing radiation is also connected with possible occurrences of accidents and emergencies. Such events can then cause damage to a person health or the environment . The usage of sources of ionizing radiation belongs to those few human activities in the world, where a big attetion is paid not only to the development of appli-cations but also to their safety. However, despite all of it, it is not possible to eliminate the risk of accidents and emergencies. Recently, there has been some talk of possible misuses of the sources of ionizing radiation. Such extraordinary events place high on the first response forces. One of the first re-sponse forces is Fire Rescue Service of the Czech Republic, the forces of which are lo-cated over the Cezch Republic. This thesis shall provide these forces with such infor-mation that they should be able, along with the authorities responsible for the radiation protection, to evaluate the situation and minimise effects on health, lives and environ-ment in the event of radiation extraordinary event. The purpose of this thesis is to solve local extraordinary radiation events (such as acci-dents during the transport of the sources of ionizing radiation, discovery of an unknown radiator). It does not deal with nuclear emergencies and accidents of other equipment, where the scope of the event and its consequences are of nation-wide importance. The first part of the thesis includes basic terms of the ionizing radiation and radiation pro-tection. The other part is focused on the radiation research, monitoring, response system and dosimetry devices. This part also includes materials for trainings and drills focusing on early identification of raiation hazards of an extraordinary event.
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