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Different perspectives when working with a printed textbook and an I-textbook in selected chapters of mathematics in tertiary education
VOCETKOVÁ, Klára
The Czech education market at the primary and secondary level offers a wide variety of printed textbooks, which are more and more accompanied by I-textbooks and also by hybrid textbooks. Elementary school pupils and their counterparts in secondary schools may practice their mathematical skills using various mathematical exercises, which help them achieve a solid foundation necessary for successfully completing university mathematics courses. The teaching materials for the university level are not issued widely, as it is for elementary and secondary schools, universities usually use their materials, and the university teachers recommend them to their students. Some of the teachers present to their students printed practice books, and others use digital teaching portals available online. Seldomly the teachers provide the teaching materials to their students according to their individual learning styles. This approach is a core issue of the presented research. The thesis consists of two parts: theoretical and empirical. The theoretical part presents the theoretical background of the research, including the definition and classification of textbooks and the functional structure textbook analysis and categorization of the mathematical text. A particular chapter is devoted to the university education level, specifics of the mathematical text, and the didactical principles in the context of textbook selection. The empirical part presents the conducted research methodology following the results of previous pieces of research in variable conditions. The main objective of the work is to identify the different viewpoints on the work with a printed university mathematics textbook and an I-textbook. The main viewpoints are the number of used hints necessary for solving mathematical problems, the students' error rate, and the time the students need to solve these problems. Other research questions deal with the selection preferences of the students regarding the media and the utility of interactive elements in new media. The research respondents are first-year university students participating in the introductory course of engineering Mathematics at the Faculty of Economics at the University of South Bohemia in České Budějovice who were given several sets of mathematical problems to solve and to help them to acquire the given subject matter. These problems were selected based on a specification table to meet the necessary validity, reliability, practicability, and sensitivity so that the posed research questions and formulated hypotheses could be processed quantitatively and verified.
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Intelligent nanofibres functionalized with growth factors and blood derivatives for dermatology applications
Vocetková, Karolína ; Amler, Evžen (advisor) ; Rosina, Jozef (referee) ; Arenberger, Petr (referee)
Platelet derivatives are an attractive source of natural growth factors and they are widely used in various tissue engineering and regenerative medicine applications. The aim of this study was to optimize cell culture conditions using platelet lysate and to develop platelet-functionalized fibrous scaffolds as a controlled drug delivery system for native growth factors. Fibrous scaffolds were prepared by electrostatic and centrifugal spinning of PCL and they were functionalized by the platelets by surface adhesion or their encapsulation using emulsion spinning techniques. The cell culture study determined the 7% platelet lysate to be the optimum concentration as a medium supplement in keratinocyte and fibroblast culture. Additionally, following surface adhesion of the platelets to PCL electrospun nanofibres, the platelets were activated due to their contact with the nanofibre nanotopography, resulting in formation of fibrin network. Fibrin served as a reservoir of the growth factors, prolonging the half-time of EGF release to 1.7 days. Such platelet-functionalized samples fostered proliferation of keratinocytes, fibroblasts and melanocytes. Furthermore, adhesion of platelets to centrifugally spun nanofibrous scaffolds resulted in almost two-fold increase in the amount of immobilized platelet-derived...
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Nanofiber dressing consisting of antisense rna-functionalized nanodiamonds for therapy of non-healing wounds in diabetic individuals
Neuhoferová, Eva ; Petráková, V. ; Vocetková, K. ; Kindermann, Marek ; Křivohlavá, Romana ; Benson, Veronika
Non-healing wounds are serious complication in diabetic patients and represent an attractive challenge for development of suitable carrier system possessing constant and localized release of therapeutic biomolecule into the wound without any undesired side effects. Given the fact that these non-healing wounds are result of impaired balance in metalloproteinases synthesized by immune cells residing the wounds, gene therapy offering knock down of such enzymes is of great interest. \nHere we challenged a development of functional and biocompatible wound dressing enabling controlled release of trackable carrier loaded with therapeutic siRNA. Our dressing consists of scaffold from degradable polymer nanofibers enriched with fluorescent nanodiamond particles (FND). We have previously shown the nanodiamond particles are great carriers for antisense RNAs. Their advantages represent high biocompatibility, stable luminescence giving us the possibility to track the carrier system in the wound, and effective release of antisense RNA in the wound. Embedding of nanodiamond-siRNA systems into nanofiber scaffold enables continuous release of siRNA and maintaining the stable siRNA concentration in the wound site resulting in a promotion of wound healing. \nWe developed FND-siRNA complexes specific to MMP-9 that efficiently inhibit the expression of target MMP-9 mRNA. The complexes were embedded into core/shell nanofibers from PVA and PCL, visualized by confocal microscopy, and characterized by electron microscopy. Real-time PCR was used to assess the silencing effect of siRNA that has been delivered to target murine fibroblasts by FND released from nanofiber dressing. Nanofiber system with embedded FNDs was applied on wounds in diabetic animal models to evaluate its suitability regarding short and long term toxicity, efficacy, and handling in vivo. \n
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