|
|
Use of Kluyveromyces marxianus to bioethanol produce from waste paper
Tomečková, Andrea ; Hrstka, Miroslav (referee) ; Babák, Libor (advisor)
The diploma thesis is focused on production possibilities of bioethanol from waste paper by yeast Kluyveromyces marxianus. Waste cardboard was used as a potential substrate for bioethanol production. Several methods for cardboard preparation were introduced and compared as well as methods of fermentation. Simultaneous sacharification and fermentation and separate hydrolysis and fermentation of preprepared cardboard paper were performed in different pH buffer (4,8-7). Simultaneous sacharification and fermentation was held at a temperature of 45°C. Hydrolysis in separate hydrolysis and fermentation was performed at 50°C and fermentation at 25°C. Procedures outputs were obtained by sampling in specific time intervals and samples were analyzed by HPLC for presence and concentration glucose and ethanol. The results of the analysis have shown that the highest concentration of glucose produced by enzymatic hydrolysis was achieved by using microwaves, 2% H2SO4 and 2% NaOH pretreated paperboard at pH 4,8. The highest yield of ethanol was obtained by separate hydrolysis and fermentation of pulp pretreated by microwaves, 2% H2SO4 and 2% NaOH in pH 5,4 buffer. The method SHF proved to be more effective for the production of ethanol than SSF.
|
|
|
Use of Kluyveromyces marxianus to bioethanol produce from waste paper
Tomečková, Andrea ; Hrstka, Miroslav (referee) ; Babák, Libor (advisor)
The diploma thesis is focused on production possibilities of bioethanol from waste paper by yeast Kluyveromyces marxianus. Waste cardboard was used as a potential substrate for bioethanol production. Several methods for cardboard preparation were introduced and compared as well as methods of fermentation. Simultaneous sacharification and fermentation and separate hydrolysis and fermentation of preprepared cardboard paper were performed in different pH buffer (4,8-7). Simultaneous sacharification and fermentation was held at a temperature of 45°C. Hydrolysis in separate hydrolysis and fermentation was performed at 50°C and fermentation at 25°C. Procedures outputs were obtained by sampling in specific time intervals and samples were analyzed by HPLC for presence and concentration glucose and ethanol. The results of the analysis have shown that the highest concentration of glucose produced by enzymatic hydrolysis was achieved by using microwaves, 2% H2SO4 and 2% NaOH pretreated paperboard at pH 4,8. The highest yield of ethanol was obtained by separate hydrolysis and fermentation of pulp pretreated by microwaves, 2% H2SO4 and 2% NaOH in pH 5,4 buffer. The method SHF proved to be more effective for the production of ethanol than SSF.
|
|
|
Physiological parameters Suffolk breed of sheep breeding in Soběslav
TOMEČKOVÁ, Andrea
The aim of the thesis was the evaluation of commercial properties, physiological state and selected blood parameters in sheep suffolk breed with focus on monitoring increases, blood parameters and coprological examination which prooved parasitological settlement. Monitoring was conducted in 2013 and early 2014 when 17 ewes and 16 lambs were observed. For thesis purpose two samples were made on 25.3.2013 and 14.10.2013. Several elevated values have been detected among which were glucose, urea, total protein, albumin, 1 and 2 globulin and partially cholesterol. ß globulin was below the physiological range, globulin was initially below the physiological range, but in the second sampling was above the physiological range. Decreased values were observed in case of calcium concentration in blood plasma. The parasitological findings prooved presence of coccidia Eimeria and gastrointestinal nematodes.
|
|
| |
guest ::
RSS feed.