|
|
Elicitation of bioactive compounds production in in vitro cultures of Panax ginseng
Syslová, Eliška ; Skálová, Lenka (advisor) ; Karlíčková, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Eliška Syslová Supervisor: prof. RNDr. Lenka Skálová, Ph.D. Thesis title: Elicitation of bioactive compounds production in in vitro cultures of Panax ginseng This diploma thesis is focused on the study of affecting the biosynthesis of secondary metabolites of Panax ginseng C. A. Meyer and is focused on increasing the production of biologically active polyacetylenes and ginsenosides in in vitro root cultures by various elicitors. The effect of the concentration of elicitors and growth regulators on the production of root biomass and secondary metabolites was researched. The carriers of the main pharmacological actions of Panax ginseng are ginsenosides and polyacetylenes. The root cultures were cultivated in the liquid medium in the dark at 24 ± 1řC. During the 27 day the sterile elicitation was done and a week later it was harvested. The samples were processed according to the optimized procedure, separately for UPLC analysis of ginsenosides and UPLC analysis of polyacetylenes as well. The presence of individual elicitors influenced the growth of biomass and the amount of ginsenosides and polyacetylenes in the samples.
|
|
|
Anthelmintics in plants - uptake, biotransformation and transcriptional response
Syslová, Eliška ; Szotáková, Barbora (advisor) ; Petrová, Šárka (referee) ; Tůmová, Lenka (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate Mgr. Eliška SYSLOVÁ Supervisor prof. Ing. Barbora SZOTÁKOVÁ, Ph.D. Consultant RNDr. Radka PODLIPNÁ, Ph.D. Title of Doctoral Thesis: Anthelmintics in Plants - Uptake, Biotransformation and Transcriptional Response The use of anthelmintics, drugs against parasitic worms, is almost necessary in veterinary medicine today. However, anthelmintics may enter the environment with the excrements of treated animals, and influence non-target organisms there. Plants which can receive, biotransform or accumulate these substances, are an integral part of the environment. Biotransformation of anthelmintics may affect the capacity of the antioxidant system, interfere with endogenous plant metabolism or polyphenol biosynthesis where plants use the same detoxifying enzymes. The aim of this thesis is to study the influence of the most commonly used anthelmintics albendazole (ABZ), fenbendazole (FBZ), flubendazole (FLU) and ivermectin (IVM) on plants. To study their uptake by plants, their biotransformation in plants and transcriptional response of plant cells to their presence. The results of the dissertation include the finding that model systems of cell suspensions and in vitro regenerants of Plantago lanceolata...
|
|
|
Anthelmintics in plants - uptake, biotransformation and transcriptional response
Syslová, Eliška ; Szotáková, Barbora (advisor) ; Petrová, Šárka (referee) ; Tůmová, Lenka (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate Mgr. Eliška SYSLOVÁ Supervisor prof. Ing. Barbora SZOTÁKOVÁ, Ph.D. Consultant RNDr. Radka PODLIPNÁ, Ph.D. Title of Doctoral Thesis: Anthelmintics in Plants - Uptake, Biotransformation and Transcriptional Response The use of anthelmintics, drugs against parasitic worms, is almost necessary in veterinary medicine today. However, anthelmintics may enter the environment with the excrements of treated animals, and influence non-target organisms there. Plants which can receive, biotransform or accumulate these substances, are an integral part of the environment. Biotransformation of anthelmintics may affect the capacity of the antioxidant system, interfere with endogenous plant metabolism or polyphenol biosynthesis where plants use the same detoxifying enzymes. The aim of this thesis is to study the influence of the most commonly used anthelmintics albendazole (ABZ), fenbendazole (FBZ), flubendazole (FLU) and ivermectin (IVM) on plants. To study their uptake by plants, their biotransformation in plants and transcriptional response of plant cells to their presence. The results of the dissertation include the finding that model systems of cell suspensions and in vitro regenerants of Plantago lanceolata...
|
|
|
Elicitation of bioactive compounds production in in vitro cultures of Panax ginseng
Syslová, Eliška ; Skálová, Lenka (advisor) ; Karlíčková, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Eliška Syslová Supervisor: prof. RNDr. Lenka Skálová, Ph.D. Thesis title: Elicitation of bioactive compounds production in in vitro cultures of Panax ginseng This diploma thesis is focused on the study of affecting the biosynthesis of secondary metabolites of Panax ginseng C. A. Meyer and is focused on increasing the production of biologically active polyacetylenes and ginsenosides in in vitro root cultures by various elicitors. The effect of the concentration of elicitors and growth regulators on the production of root biomass and secondary metabolites was researched. The carriers of the main pharmacological actions of Panax ginseng are ginsenosides and polyacetylenes. The root cultures were cultivated in the liquid medium in the dark at 24 ± 1řC. During the 27 day the sterile elicitation was done and a week later it was harvested. The samples were processed according to the optimized procedure, separately for UPLC analysis of ginsenosides and UPLC analysis of polyacetylenes as well. The presence of individual elicitors influenced the growth of biomass and the amount of ginsenosides and polyacetylenes in the samples.
|
|
|
Transition to Democracy in Rwanda with Regard to the Role of Chieftaincy
Syslová, Eliška ; Mlejnek, Josef (advisor) ; Skalník, Petr (referee)
This diploma thesis "Transition to democracy in Rwanda with Regard to the Role of Chieftaincy" analyses the democratization process in Rwanda, which had run before the genocide as well as after the genocide in 1994. This diploma thesis draws from theoretical conceptions of democratization, which are introduced in the first chapter. In order to understand the circumstances in which the transition to democracy is taking place, the history of Rwanda since the beginning of colonization until the first republic is analysed. In the following period, the influence of important elements forming the shape of the second republic regime is depicted. The essence of this thesis is the analysis of the democratization processes, which genesis are to be found in the second half of the 1980's. The democratization and attitudes of individual participants are analysed until 2003, when a new constitution was approved. The events of the genocide are described briefly. The final part deals with clientship and chieftaincy and their role during the democratization process. The theoretical basis of the analysis derives from the work of Max Weber. The importace of clientship after 1994 is analysed on the gacaca tribunals. Powered by TCPDF (www.tcpdf.org)
|
|
| |
guest ::
