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The effect of mebendazole on proliferation, migration and adhesion of oral cancer cells in vitro
Stupková, Eva ; Skálová, Lenka (advisor) ; Boušová, Iva (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Eva Stupková Supervisor: RNDr. Věra Králová, Ph.D. Title of diploma thesis: Effect of mebendazole on proliferation, migration and adhesion of oral carcinoma cells in vitro Mebendazole is one of commonly used anthelmintics in both human and veterinary medicine. The mechanism of its action is based on microtubule synthesis inhibition. This thesis is focused on mebendazole effect on cancer cells. Especially, we studied the effect of mebendazole on proliferation, migration and adhesion of squamous-cell carcinoma (SCC) cells, very aggressive and highly invasive cancerous disease. Our experiments ran on two cancerous cell lines (DOK, PE/CA-PJ15) and on non-cancerous gingival fibroblasts. We used WST-1 method for proliferation change assessment and xCELLigence RTCA DP Analyzer for measuring the effect on migration and adhesion of SCC cells. In our experiments, mebendazole caused decrease of SCC proliferation, migration and it also had effect on adhesion, in clinically achievable concentrations.
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Products of Black Eagle Pharmacy at Malá Strana district of Prague
Stupková, Eva ; Babica, Jan (advisor) ; Dohnal, František (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Social and Clinical Pharmacy Candidate: Mgr. Eva Stupková Supervisor: PharmDr. Jan Babica, Ph.D. Title of rigorosum thesis: Products of Black Eagle Pharmacy at Malá Strana district of Prague This thesis is focused on products of Black Eagle Pharmacy in years 1857-1930. Throughout these years subsequently Benjamin, Karel and Jiří Fragner were the head pharmacists. The pharmacy offered many products in various forms (the oldest ones include Prague Universal Household Ointment or Dr. Rosa's Balsam). At the beginning of the 20th century the range of goods was even wider. The pharmacy produced medi- cinal products (such as Buccavacedrol, Galegol, etc.) as well as dairy products, sweets and other goodies. In the 20's the variety of products increased to such a degree that the production had to be moved to a B. Fragner factory in Dolní Měcholupy. Some of the medicinal products were still made by the end of 20th century (Jopton, Guanar, Hysteps, etc.). In some instances the name has changed but the contents remained close to the original up to this day (Thymomel). Core of this thesis is a chronologically ordered list of products. Each product has its own brief characteristic, contents, indication and dosage information. For the sake of...
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Products of Black Eagle Pharmacy at Malá Strana district of Prague
Stupková, Eva ; Babica, Jan (advisor) ; Dohnal, František (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Social and Clinical Pharmacy Candidate: Mgr. Eva Stupková Supervisor: PharmDr. Jan Babica, Ph.D. Title of rigorosum thesis: Products of Black Eagle Pharmacy at Malá Strana district of Prague This thesis is focused on products of Black Eagle Pharmacy in years 1857-1930. Throughout these years subsequently Benjamin, Karel and Jiří Fragner were the head pharmacists. The pharmacy offered many products in various forms (the oldest ones include Prague Universal Household Ointment or Dr. Rosa's Balsam). At the beginning of the 20th century the range of goods was even wider. The pharmacy produced medi- cinal products (such as Buccavacedrol, Galegol, etc.) as well as dairy products, sweets and other goodies. In the 20's the variety of products increased to such a degree that the production had to be moved to a B. Fragner factory in Dolní Měcholupy. Some of the medicinal products were still made by the end of 20th century (Jopton, Guanar, Hysteps, etc.). In some instances the name has changed but the contents remained close to the original up to this day (Thymomel). Core of this thesis is a chronologically ordered list of products. Each product has its own brief characteristic, contents, indication and dosage information. For the sake of...
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The effect of mebendazole on proliferation, migration and adhesion of oral cancer cells in vitro
Stupková, Eva ; Skálová, Lenka (advisor) ; Boušová, Iva (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Eva Stupková Supervisor: RNDr. Věra Králová, Ph.D. Title of diploma thesis: Effect of mebendazole on proliferation, migration and adhesion of oral carcinoma cells in vitro Mebendazole is one of commonly used anthelmintics in both human and veterinary medicine. The mechanism of its action is based on microtubule synthesis inhibition. This thesis is focused on mebendazole effect on cancer cells. Especially, we studied the effect of mebendazole on proliferation, migration and adhesion of squamous-cell carcinoma (SCC) cells, very aggressive and highly invasive cancerous disease. Our experiments ran on two cancerous cell lines (DOK, PE/CA-PJ15) and on non-cancerous gingival fibroblasts. We used WST-1 method for proliferation change assessment and xCELLigence RTCA DP Analyzer for measuring the effect on migration and adhesion of SCC cells. In our experiments, mebendazole caused decrease of SCC proliferation, migration and it also had effect on adhesion, in clinically achievable concentrations.
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