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3
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Differentiation of Sertoli cell progenitors and preparation of testicular 3D cultures of Xenopus tropicalis.
Slováková, Lucie
;
Tlapáková, Tereza
(advisor) ;
Hovořáková, Mária
(referee)
Sertoli cells represent the only somatic cell type within the seminiferous tubules with direct contact to germ cells. Sertoli cells significantly contribute to the development of the testicular niche in a male embryo. Their role during postnatal life is in the regulation and nutrition of germ cells and the formation of the blood-testis barrier to protect these cells. In our laboratory, we have been successful in establishing a cell line of X. tropicalis immature Sertoli cells (XtiSCs) derived from juvenile testes of X. tropicalis. The objective of this thesis was to induce the differentiation process of XtiSCs into mature Sertoli cells. In vitro experiments using several factors or primary culture from adult male X. tropicalis did not show any mature markers in differentiated XtiSCs. Another experiment using cell culture derived from pubertal mice was partially successful in the induction of the differentiation process. These results indicate that XtiSCs do have some differentiation potential into mature Sertoli cells. Part of this work was to test the ability of testicular cells isolated from juvenile males of X. tropicalis to form de novo organoids. In vitro experiments were successful when these cells were cultured in a three-layer matrigel.
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The role of morphogenes in stem cell neurogenic differentiation in mammals
Slováková, Lucie
;
Tlapáková, Tereza
(advisor) ;
Kyclerová, Hana
(referee)
Stem cells are non-differentiated self-renewing cell population that can derive different kinds of cell types according to their differential potential. Neurogenic differentiation is the process of generating of all three types of nervous systems from the neural stem cells. This process is common for embryonic development, however neurogenesis appears to be present also in adult mammalian brain. It continues to generate new neurons within its microenvironments called niches and we can find two major areas of neurogenesis. One is the subventricular zone of the forebrain, the other is the subgranular zone within the hippocampal dental gyrus. In these niches we can find specific signaling molecules called morphogens. Morphogens function in regulating neural stem cell activity. They play a part in proliferation, differentiation and cell migration, thus determining the fate of neural cells. In addition, morphogens play an important role in many diseases and cancers.
Detailed record
The awareness of czech adolescents about gender issues.
SLOVÁKOVÁ, Lucie
This thesis aimed at mapping out the knowledge of Czech adolescent of gender questions and establishing how they evaluate the present educational concept in regard of gender equality. As this bachelor´s thesis´ main method of research, the secondary data analysis has been chosen is the content analysis of documents regarding the gender problems. For the primary data collection, the interrogation method{--}the questionnaire technique{--}has been chosen. By means of questionnaries which had been distributed to adolescent in various school types the target has been achieved and the foreward supposition has been confirmed as well.
Detailed record
See also: similar author names
3
SLOVÁKOVÁ, Lucie
3
Slováková, Lenka
1
Slováková, Linda
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