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Increasing plant drought resistance by priming methods: the role of antioxidant capacity changes
Popelářová, Anna ; Lipavská, Helena (advisor) ; Lhotáková, Zuzana (referee)
When there is a lack of water in the soil, the plant is subject to stress during which reactive oxygen species (ROS) are formed and the water content in the tissues decreases. ROS cause oxidative damage, which the plant tries to prevent by antioxidation systems action. The osmotic adjustment process ensures water maintenance in the plant. Priming can be thought of as a treatment of plants aimed at increasing stress resistance. This thesis is mainly focused on summarizing information related to priming in connection with drought tolerance improvement and related changes in the activities and amount of antioxidation systems and osmolytes. So far, seed priming methods are probably the most explored, these include hydropriming, osmopriming and priming with elicitors. Priming in the vegetative phase of growth is mainly associated with the application of elicitors. Transgenerational priming has an impact on the progeny of treated plants. Priming-induced changes are species-specific and in some cases may affect the plants for a longer period.
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Dynamics of ALBA proteins in Arabidopsis thaliana evaluated by fluorescence microscopy
Popelářová, Anna ; Honys, David (advisor) ; Vosolsobě, Stanislav (referee)
ALBA proteins were discovered in Archaea more than 30 years ago. They were gradually identified to be well conserved in Eucaryotes as well. A functional dimeric form of these proteins with DNA and RNA-binding capability was claimed in both mentioned domains of organisms. However, their roles diversified during evolution and vary in between organisms. In Archaea, ALBAs are involved in the genome organization and RNA-protein interactions. In Eukaryotes, there are presented two different subfamilies of ALBA proteins - Rpp20 and Rpp25 subfamily. A sole protein from each subfamily was identified in some organisms though they were multiplied in plants, respectively. These proteins can interact with each other and participate in ontogenetic development and stress responses. According to several studies, ALBA proteins were found to be involved in DNA stability maintenance or pre-rRNA splicing in the nucleus of Arabidopsis thaliana. However, they have been shown to play a role in the cellular metabolism and stress responses in cytoplasm. Six ALBA proteins were identified in the genome of A. thaliana, three from each subfamily. In this study, all heterodimeric protein- protein interactions were investigated by the bimolecular fluorescence complementation (BiFC) assay which revealed positive results in...
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Increasing plant drought resistance by priming methods: the role of antioxidant capacity changes
Popelářová, Anna ; Lipavská, Helena (advisor) ; Lhotáková, Zuzana (referee)
When there is a lack of water in the soil, the plant is subject to stress during which reactive oxygen species (ROS) are formed and the water content in the tissues decreases. ROS cause oxidative damage, which the plant tries to prevent by antioxidation systems action. The osmotic adjustment process ensures water maintenance in the plant. Priming can be thought of as a treatment of plants aimed at increasing stress resistance. This thesis is mainly focused on summarizing information related to priming in connection with drought tolerance improvement and related changes in the activities and amount of antioxidation systems and osmolytes. So far, seed priming methods are probably the most explored, these include hydropriming, osmopriming and priming with elicitors. Priming in the vegetative phase of growth is mainly associated with the application of elicitors. Transgenerational priming has an impact on the progeny of treated plants. Priming-induced changes are species-specific and in some cases may affect the plants for a longer period.
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