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Analýza parametrů sazenic lesních dřevin z hlediska atraktivity pro prase divoké (Sus scrofa L.)
Petrů, Markéta
Increasing population of wild boar correlate with increasing damage not only on agricultural lands on crops or grasslands, but also in forests. This damage is mainly on new forest tree plantations which in no way contributes to the successful restoration of clearings after the bark beetle calamity and has a negative impact on the economy. This diploma thesis describes possible influences that may affect the plants attractiveness for wild boar and their damage. An inventory of plants damage was carried out on randomly selected areas in the areas of southern, central and eastern Moravia. A series of experiments with plantings was also performed on the experimental area in the Moravský Krumlov game reserve, where the behaviour of pigs towards these plantings was monitored by the analysis of damaged seedlings and also from pictures taken by photo traps. Obtained inventory data showed us that the wild boar preferred containerized seedlings and deciduous tree species however the experimental system planting did not show a preference for the root system. Seedlings damage was demonstrated in the vast majority only by pulling or rooting without damaging the root system. In terms of time the seedlings were most damaged in the first week after planting, then the risk of damage decreased. To reduce the risk of disturbance by pigs on plantings a method of protection against this damage is not known yet, so the most effective solution remains to reduce the population of wild boar in affected areas.
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Bacterial proteins in the biogenesis of mitochondria of unicellular eukaryotes.
Petrů, Markéta ; Doležal, Pavel (advisor) ; Embley, Martin (referee) ; Hashimi, Hassan (referee)
in English Formation of mitochondria by the conversion of a bacterial endosymbiont is the fundamental moment in the evolution of eukaryotes. An integral part of the organelle genesis was the displacement of the endosymbiont genes to host nucleus and simultaneous creation of new pathways for delivery of proteins synthesized now in the host cytoplasm. Resulting protein translocases are complexes combining original bacterial components and eukaryote-specific proteins. In addition to these novel protein import machines, some components of the original bacterial secretory pathways have remained in the organelle. While the function of a widely distributed mitochondrial homolog of YidC, Oxa1, is well understood, the role of infrequent components of Sec or Tat translocases has not yet been elucidated. So far, more attention has been paid to their abundant plastid homologs, which assemble photosynthetic complexes in the thylakoid membrane. In the thesis, the structure and function of prokaryotic YidC, Sec and Tat machineries and their eukaryotic homologs are described. By comparing both organelles of the endosymbiotic origin, the hypothesis is drawn on why these translocases have been more "evolutionary successful" in plastids than in mitochondria.
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How Intracellular Pathogens Manipulate Cellular Trafficking.
Petrů, Markéta ; Doležal, Pavel (advisor) ; Pyrih, Jan (referee)
Many intracellular single-celled organisms belong to medically important human pathogens. The selected parasites are subject of this thesis - Chlamydia spp., Legionella pneumophila, Trypanosoma cruzi and Toxoplasma gondii - as well as their interactions with the vesicular transport of the host cell. Basic pathways of vesicular transport are delineated and important participating molecules described. Furthermore, the effector proteins of pathogens that interact with these molecules are included. The special chapter is devoted to phenomenon of mimetics of SNARE proteins by bacteria. The manuscript concludes with a chapter on LpSNARE of Legionella pneumophila, which was found in our laboratory and which is a topic of my experimental work herein.
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The inner mitochondrial membrane cristae biogenesis
Efimova, Iuliia ; Mráček, Tomáš (advisor) ; Petrů, Markéta (referee)
Invaginations of the inner mitochondrial membrane originate cristae - important structural and bioenergetic mitochondrial compartments. Long-term observations of mitochondrial ultrastructure uncovered cristae dynamics, but did not identify mechanisms of cristae formation and maintenance. This thesis summarizes results of latest research on molecular mechanisms of mitochondrial cristae biogenesis, which are conserved from fungi to mammals including human. The emphasis is put on major remodeling factors: F1Fo-ATP synthase dimers, MICOS complex, OPA1 protein and cardiolipin. Their defects lead to extensive changes on cristae level, as well as on mitochondrial, cellular and organismal levels. Various pathophysiological conditions and human mitochondrial diseases are related to these defects. More detailed research of cristae biogenesis is therefore of high significance, new findings could assist in the development of new treatments for mitochondrial disorders.
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Bacterial proteins in the biogenesis of mitochondria of unicellular eukaryotes.
Petrů, Markéta
in English Formation of mitochondria by the conversion of a bacterial endosymbiont is the fundamental moment in the evolution of eukaryotes. An integral part of the organelle genesis was the displacement of the endosymbiont genes to host nucleus and simultaneous creation of new pathways for delivery of proteins synthesized now in the host cytoplasm. Resulting protein translocases are complexes combining original bacterial components and eukaryote-specific proteins. In addition to these novel protein import machines, some components of the original bacterial secretory pathways have remained in the organelle. While the function of a widely distributed mitochondrial homolog of YidC, Oxa1, is well understood, the role of infrequent components of Sec or Tat translocases has not yet been elucidated. So far, more attention has been paid to their abundant plastid homologs, which assemble photosynthetic complexes in the thylakoid membrane. In the thesis, the structure and function of prokaryotic YidC, Sec and Tat machineries and their eukaryotic homologs are described. By comparing both organelles of the endosymbiotic origin, the hypothesis is drawn on why these translocases have been more "evolutionary successful" in plastids than in mitochondria.
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Porovnání růstu prostokořenného a krytokořenného sadebního materiálu
Petrů, Markéta
This work is about comparison of bare-rooted and containerized planting stocks on 5 locations with different conditions, which were established for the purpose of this kind of research. Locations consisted of these forest type sets: 4K, 4S, 5K and two areas with a forest type set 3K. There were 200 bare-rooted varieties and 200 containerized varieties of three species planted on these locations in 2016, namely European beech (Fagus sylvatica L.), Douglas fir (Pseudotsuga menziesii (Mirb.) Franco) and Norway spruce (Picea abies (L.) Karsten). After the end of the vegetation period in 2017, measurements were made on the mentioned trees. Hundred individual trees from each variant were measured. The following parameters and features were measured: height of the aboveground part in 2017, height of the aboveground part in 2016, length of the growth of branches, thickness of the root neck, width of the crown, length and width of the assimilation apparatus, ripple of the trunk, diversion of the trunk, height of split of multiple trunk, colour of the assimilation apparatus, crown shape, multiple top, losses. The measurements were further statistically evaluated and the results showed that the containerized variant of European beech has better growth at all evaluated locations. Containerized variant of all plants was better on 5K area than on the other areas. Bare-rooted variant of Norway spruce had better growth on forest type sets such as 3K,3K B,4K, and 4S. Bare-rooted variant of Douglas fir had better results on 3K (Svárov) and 4K areas, but containerized variant had better results on 3K (Vanovice) and 4K area.
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Bacterial proteins in the biogenesis of mitochondria of unicellular eukaryotes.
Petrů, Markéta
in English Formation of mitochondria by the conversion of a bacterial endosymbiont is the fundamental moment in the evolution of eukaryotes. An integral part of the organelle genesis was the displacement of the endosymbiont genes to host nucleus and simultaneous creation of new pathways for delivery of proteins synthesized now in the host cytoplasm. Resulting protein translocases are complexes combining original bacterial components and eukaryote-specific proteins. In addition to these novel protein import machines, some components of the original bacterial secretory pathways have remained in the organelle. While the function of a widely distributed mitochondrial homolog of YidC, Oxa1, is well understood, the role of infrequent components of Sec or Tat translocases has not yet been elucidated. So far, more attention has been paid to their abundant plastid homologs, which assemble photosynthetic complexes in the thylakoid membrane. In the thesis, the structure and function of prokaryotic YidC, Sec and Tat machineries and their eukaryotic homologs are described. By comparing both organelles of the endosymbiotic origin, the hypothesis is drawn on why these translocases have been more "evolutionary successful" in plastids than in mitochondria.
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Bacterial proteins in the biogenesis of mitochondria of unicellular eukaryotes.
Petrů, Markéta ; Doležal, Pavel (advisor) ; Embley, Martin (referee) ; Hashimi, Hassan (referee)
in English Formation of mitochondria by the conversion of a bacterial endosymbiont is the fundamental moment in the evolution of eukaryotes. An integral part of the organelle genesis was the displacement of the endosymbiont genes to host nucleus and simultaneous creation of new pathways for delivery of proteins synthesized now in the host cytoplasm. Resulting protein translocases are complexes combining original bacterial components and eukaryote-specific proteins. In addition to these novel protein import machines, some components of the original bacterial secretory pathways have remained in the organelle. While the function of a widely distributed mitochondrial homolog of YidC, Oxa1, is well understood, the role of infrequent components of Sec or Tat translocases has not yet been elucidated. So far, more attention has been paid to their abundant plastid homologs, which assemble photosynthetic complexes in the thylakoid membrane. In the thesis, the structure and function of prokaryotic YidC, Sec and Tat machineries and their eukaryotic homologs are described. By comparing both organelles of the endosymbiotic origin, the hypothesis is drawn on why these translocases have been more "evolutionary successful" in plastids than in mitochondria.
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The invasion apparatus of protozoan parasites
Novotná, Veronika ; Petrů, Markéta (advisor) ; Štáfková, Jitka (referee)
Protozoa parasitism has evolved many times independently in the history of eukaryotes. Some organelles were reduced, and specific invasion apparatuses were developed by adapting to the host. These apparatuses help to penetrate host cells and escape the immune system. The thesis summarizes the findings of the invasion apparatus and invasion of four selected unicellular protozoan parasites of genus Plasmodium, Microsporidia, Ichthyobodo and Rozella. Each chapter includes general knowledge of the parasite, its structure and life cycle. Keywords: Apicomplexa, Plasmodium, Microsporidia, Ichthyobodo, Rozella, merozoite, glideosom, moving junction, polar tube, germination, cytostome, zoospore, germ tube;
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Nosema bombycis as a model organism for polar tube study.
Satrapová, Alexandra ; Petrů, Markéta (advisor) ; Trdá, Lucie (referee)
Microsporidia is a group of obligatory intracellular parasites with a unique invasion mechanism. One of the highly specialized invasion structures is the polar tube which is an example of a biological nanotube the study of which could bring usable knowledge in biotechnology. The thesis is a literary overeview which deals with the structure of microsporidian spores and presents N. bombycis as a suitable representative for the study of the infectious apparatus. In more detail, it summarizes the background to the structure of the polar tube and wall of N. bombycis spore.
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