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Sequential analysis of the LDL receptor gene
MAŠKOVÁ, Denisa
Attention is currently being paid to the LDL (low density lipoprotein) receptor. It was discovered in 1985 by Goldstein and Brown who discovered its function and pathogenesis of familial hypercholesterolemia. The receptor gives a cell enough cholesterol essential to membrane synthesis and attend as a precursor to some products of cells (for example bile acids and sterols) (Ashermann et al., 2004). The mutation that affect the LDL receptor (LDLR) gene indicate some disorder of LDLR functions (Češka, 2005). The result of the disorders are increased of plasma LDL cholesterol and the associated early development of atherosclerosis and other disorders (Khan, 2014). This bachelor thesis deals with the analysis of the LDLR gene. The gene is located on the short arms of chromosome 19 in region 13.2, only exon 15 was analyzed. Identification of mutations in the LDLR gene was performed by Sanger sequencing. The sequences were processed in EditBio program and compared with the NCBI (National Center for Biotechnology Information). The methodical part was performed on a set of 15 anonymized DNA samples in which one pathological mutation (c.2253_2256dupGCTG) was found causing familial hypercholesterolemia, one probably benign (c.2225C>T), the most frequent was benign mutation (c.2232A>G) and one mutation in the intron was also found. Only one sample was without mutation.
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Analysis of the current opposition in Russia
Mašková, Denisa ; Dvořáková, Vladimíra (advisor) ; Klokava, Anfisa (referee)
The thesis focuses on analysis of the current opposition in Russia. Attention is mainly focused on the conditions for the existence and operation of the opposition created by the government's garniture, including the purposeful changes of the valid legislation and its influence on the state of the present opposition. Furthermore, the work aims to map key opposition parties and find out what the character of the current opposition is, whether it offers a liberal-democratic alternative to the existing regime or not. Last but not least, presented public opinion polls investigate whether there is room for opposition in society and what the society's attitude toward opposition is. This thesis is a contribution to discussion in the context of the nature of the current regime in Russia and the real possibilities for its change in the near future, because analysing the opposition is important for understanding of the system and its functioning, both in terms of conditions for its functioning, its embedding in society and ideological focus.
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Analysis of differences in the party systems of Ukraine and Russia
Mašková, Denisa ; Němec, Jan (advisor) ; Husák, Jan (referee)
The thesis focuses on the comparison of party systems of Ukraine and Russia. Attention is focused on the historical context of the development of the party systems and their changes after each parliamentary election since the collapse of the Soviet Union. The aim is to identify the causes of differences in party systems through the analysis of the institutional and legal framework and its influence on the composition of the current parliaments, stability of party systems and circumstances of the formation of political parties. The work represents a contribution to the debate about the transformation of party systems of post-communist countries, their specifics and the approaching party systems of consolidated democracies of Western Europe.
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