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Modulatory effects of blueberry extract on the activities and expression of selected biotransformation enzymes in rat
Láníčková, Tereza ; Skálová, Lenka (advisor) ; Boušová, Iva (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Biochemical Sciences Candidate: Tereza Láníčková Supervisor: Prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: Modulatory effects of bilberry extract on the activities and expression of selected biotransformation enzymes in rat Bilberry (Vaccinium myrtillus L., Ericacea) is one of the richest sources of anthocyanins. These bioactive polyphenolic secondary metabolites give bilberry not only a characteristic blue/black colour, but they are also responsible for many health benefits. Therefore, bilberry has potential use in the treatment and prevention of conditions associated with visual disorders, inflammation, dyslipidemia, hyperglycemia or in some other disorders associated with increased oxidative stress. The aim of this study was to evaluate the effect of bilberry extract, which was administrated for 29 and 58 days, on activity and expression of selected biotransformation enzymes in rat liver. Enzymatic activity and expression of mRNA was determined in the cytosols and microsomes of liver tissue from control and influenced rats. In this study, spectrophotometric or HPLC method was used to measure the activities of aldehyde reductase (AKR1A1), hydroxysteroid dehydrogenase (AKR1C), glutathione-S-transferase (GST),...
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