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Synthesis and characterization of new insulin analogs with a triazole bridge at the C-terminus of the B chain
Kuntová, Vendula ; Jiráček, Jiří (advisor) ; Ryšlavá, Helena (referee)
Insulin is a peptide hormone responsible for maintaining glucose homeostasis in the circulation. Insulin interacts with two isoforms of the insulin receptor, IR-A and IR-B, which have different tissue distribution. IR-A is supposed to have rather mitogenic function and IR-B rather metabolic function. The goal of this study was to develop insulin analog, which will be more selective for IR-B than human insulin. We prepared three new insulin analogs with a 1,2,3-triazole bridge at the positions B26 and B29. The triazole bridge was formed by Cu(I)- catalysed cycloaddition between side chains of azidopentanoic acid (N3Pent) at B26 and propargylglycine (Prg) at B29. The analogs differed in configurations on C carbons of unusual amino acids at the positions B26 and B29. Specifically, we prepared insulin analog 1 with D- N3PentB26 and D-PrgB29, insulin analog 2 with D-N3PentB26 and L-PrgB29 and insulin analog 3 with L-N3PentB26 and D-PrgB29. New analogs were tested for their binding to both isoforms (IR-A and IR-B) of the insulin receptor. Analogs 1 and 2 were less potent in binding than human insulin and had no selectivity for receptor isoforms. Analog 3 was 4-times more potent in binding to IR-B and 2-times more potent in binding to IR-A than human insulin. However, the binding selectivity of the...
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Monitoring of blood glucose level in patients with diabetes in patients with diabetes in specific situations
Kuntová, Vendula ; Matoulek, Martin (advisor) ; Váchová, Alena (referee)
The correct providing of the selfmonitoring by the diabetic patients can lead to the sufficient offset of the diabetes. Continuous monitoring of the glycaemia belongs among its most modern methods and thank to it is possible to detect even four times more serious glycaemia deviations than if we use the standard glucose measure methods. This bachelor thesis deals with the continuous glycaemia monitoring of the first type diabetes female patient. Main goal of this thesis is to describe the glycaemia changes in the case of the specific physical activities depending on the diet and daily regime providing the analysis of changes and prediction of the glycaemia. The theoretical part of my thesis summarizes the basic information regarding the diabetes mellitus disease. Subsequently, thesis is mainly focused on the acute complications of diabetes, glycaemia monitoring and options of the insuline modes. The practical part is dedicated to the evaluation of the female patient diet, physical activities and sensors records. The evaluation is provided through the organized tables and charts. The detail analysis of glycaemia was performed during the thirteen days. In the thesis were described causes of the outbreak of hyperglycaemia and hypoglycaemia depending on the nutrition, physical activities and daily...
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