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Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice
Krejcarová, Jana ; Rathouská, Jana (advisor) ; Nachtigal, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice Author: Jana Krejcarová Supervisor: PharmDr. Jana Rathouská, Ph.D. Background: This thesis was focused on the detection and subsequent evaluation of the expression of adhesion molecules ICAM-1, VCAM-1 and COX-2 and P-selectin in mouse aortas. Control and monitored group of mice differed in the genetic material, where monitored group of mice had unfunctional genes for apolipoprotein E and LDL receptor (apoE−/− /LDLR−/− ). Methods: For the study, two months old male mice of the C57BL/6J strain were used. Males were divided into two groups - control and monitored with unfunctional receptor for low- density lipoprotein and for apolipoprotein E. Both groups were fed by a standard diet for rodents. For detection and subsequent quantification of the expression of the markers VCAM-1, ICAM-1, COX-2 and P-selectin, Western blot analysis was used. Levels of soluble ICAM-1 and P-selectin in plasma were assessed by Luminex®. Results: Western blot analysis showed no significant difference in the expression of VCAM-1, ICAM-1, COX-2 and P-selectin between both groups of mice. Using the...
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Music interpreted by educationist in kindergarden
KREJCAROVÁ, Jana
The aim of this bachelor thesis is to make variety of activities with music usage interpreted by a pedagogue for kids from kindergarten. The goal of the activities is to instigate a relationship to music and a musical instrument in a kid by a pedagogue. The theoretical part deals with musical progress of preschool kid, listening to the music at this age and increase of listening skills. The musical activities and the usage of playing the musical instrument are characterized in context to contemporary education in kindergarten, the meaning of the character of a pedagogue is mentioned. The practical part deals with variety of activities with the usage of a pedagogue´s playing the piano and guitar, verifying these activities in kindergarten and subsequently assessment according to beforehand given evaluative plan. The supplements of the bachelor thesis are audio records of playing the piano of the author´s work from the realisation of the program.
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Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice
Krejcarová, Jana ; Rathouská, Jana (advisor) ; Nachtigal, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice Author: Jana Krejcarová Supervisor: PharmDr. Jana Rathouská, Ph.D. Background: This thesis was focused on the detection and subsequent evaluation of the expression of adhesion molecules ICAM-1, VCAM-1 and COX-2 and P-selectin in mouse aortas. Control and monitored group of mice differed in the genetic material, where monitored group of mice had unfunctional genes for apolipoprotein E and LDL receptor (apoE−/− /LDLR−/− ). Methods: For the study, two months old male mice of the C57BL/6J strain were used. Males were divided into two groups - control and monitored with unfunctional receptor for low- density lipoprotein and for apolipoprotein E. Both groups were fed by a standard diet for rodents. For detection and subsequent quantification of the expression of the markers VCAM-1, ICAM-1, COX-2 and P-selectin, Western blot analysis was used. Levels of soluble ICAM-1 and P-selectin in plasma were assessed by Luminex®. Results: Western blot analysis showed no significant difference in the expression of VCAM-1, ICAM-1, COX-2 and P-selectin between both groups of mice. Using the...
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Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice
Krejcarová, Jana ; Rathouská, Jana (advisor) ; Nachtigal, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice Author: Jana Krejcarová Supervisor: PharmDr. Jana Rathouská, Ph.D. Background: This thesis was focused on the detection and subsequent evaluation of the expression of adhesion molecules ICAM-1, VCAM-1 and COX-2 and P-selectin in mouse aortas. Control and monitored group of mice differed in the genetic material, where monitored group of mice had unfunctional genes for apolipoprotein E and LDL receptor (apoE−/− /LDLR−/− ). Methods: For the study, two months old male mice of the C57BL/6J strain were used. Males were divided into two groups - control and monitored with unfunctional receptor for low- density lipoprotein and for apolipoprotein E. Both groups were fed by a standard diet for rodents. For detection and subsequent quantification of the expression of the markers VCAM-1, ICAM-1, COX-2 and P-selectin, Western blot analysis was used. Levels of soluble ICAM-1 and P-selectin in plasma were assessed by Luminex®. Results: Western blot analysis showed no significant difference in the expression of VCAM-1, ICAM-1, COX-2 and P-selectin between both groups of mice. Using the...
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Nursing care of women after operative deliveries
KREJCAROVÁ, Jana
Nursing Care in Women after an Operative Delivery The bachelor thesis focuses on nursing care in women after an operative delivery terminating pregnancy. The theoretical part describes the individual surgical procedures (forceps delivery, vacuum extraction, cesarean section), nursing care and educational activity accompanying them. Further, it also includes a brief history of these methods. In the practical part, four basic goals and hypotheses were stated. The goal of the work was to identify differences in taking care of women after operative deliveries, to find out whether the care is conducted in the form of a nursing process, whether the care follows nursing and obstetrical standards in the selected delivery wards. With the view of these goals the following hypotheses were formulated: nursing care in women after operative deliveries differs in selected delivery wards and that midwives use a nursing process meeting the nursing and obstetrical standards in care of women. The research was conducted by a quantitative method. Data were collected through the use of anonymous questionnaires. These questionnaires were given to midwives in selected delivery wards (České Budějovice, Plzeň and Písek). The respondents were nurses working in delivery rooms, puerperium wards and gynecologic intensive care unit. The goals of the work were fulfilled. The hypothesis regarding the differences in care between individual delivery wards was confirmed. The second hypothesis that the care in women after operative deliveries is conducted in the form of a nursing process was proved in 72% cases. The third hypothesis confirms using of nursing standards in this care in 92% and the fourth hypothesis reveals that obstetrical standards are met in 75%. The results for the analyzed set of cases as a whole show certain definite tendencies, however the care differs among individual places and even among various wards within one institution. Therefore it would be useful to asses the organization of work, technical conditions and possible modifications that could improve the nursing care.
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