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Gamma-Tubulin forms and functions in microtubule organization and beyond
Kourová, Hana ; Binarová, Pavla (advisor) ; Šolc, Petr (referee) ; Bíšová, Kateřina (referee)
Microtubules are highly dynamic structures forming complex arrays changing accordingly to cellular requirements. In most eukaryotes, microtubules are nucleated from defined organizing centres like centrosomes or spindle pole bodies. Despite conservation of the major cytoskeletal components in plants and animals, plant cells lack centrosome-like structures and have evolved a unique mechanism to control microtubule assembly and organization. γ-Tubulin is an essential component of microtubule organizing centres, highly conserved in all eukaryotes with a prominent role in microtubule nucleation. However, growing body of evidence suggests γ-tubulin as a multifunctional protein. Next to microtubule nucleation, γ-tubulin controls microtubule plus-end dynamics, regulates gene expression, associates with DNA repair proteins and is implemented in mitotic and cell cycle regulation. We focused on γ-tubulin complexes and aimed to characterize interactors of γ-tubulin and their function in Arabidopsis. Next to characterizing microtubule dependent functions, we analyzed cell cycle and division. We also studied DNA damage response and involvement of E2F/RBR (retinoblastoma related) pathway and possible link to γ-tubulin. We characterized NodGS protein as a novel interactor of γ-tubulin in Arabidopsis and proposed...
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