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National Repository of Grey Literature

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Methodology of Diagnostics of Pectobacterium atrosepticum and Dickeya solani using Real-Time PCR. Kmoch, M. ; Binderová, D. ; Kopačka, V. ; Vacek, J. ; Brázdová, S. ; Petrzik, Karel ; Ševčík, R. The methodology describes the optimization of the molecular diagnostic method of Pectobacterium atrosepticum and Dickeya solani based on real-time PCR and SybrGreen real-time PCR, including sample preparation, DNA isolation, specificity control and evaluation. Detailed record

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