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Stability testing of benzalconium chloride stock solution by HPLC
 Křesťanová, Petra ; Matysová, Ludmila (advisor) ; Sklenářová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Petra Křesťanová Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of the diploma thesis: Monitoring the stability of benzalkonium chloride stock solution by HPLC method This diploma thesis evaluates the stability of benzalkonium chloride in aqueous stock solution. The purpose of this work was to choose the most suitable conditions for storing and determine the shelf-life. This stability study was conducted by validated High Performance Liquid Chromatography method. Tested samples were prepared with pH 6.8 and 5 and stored in glass and plastic containers at room and lowered temperature in the absence of light. The stability was analysed in intervals of 0, 7, 14, 28, 60 and 90 days. All samples showed a good stability during the whole time of testing, without any significant differences. The recommended conditions for storing benzalkonium chloride solutions are room temperature and pH 6.8 in dark glass containers. These solutions can be used in the period of three months.
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