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National Repository of Grey Literature 13 records found  previous11 - 13  jump to record: Search took 0.01 seconds. 
Evaluation of efficacy and bio-distribution of AAV5-miHTT in HD minipig brain
 Bohuslavová, Božena ; Juhás, Štefan ; Juhásová, Jana ; Ellederová, Zdeňka ; Motlík, Jan
Healthy minipigs (n=29) were pre-screened for serum AAV5 neutralizing antibodies (NABs) in serum by uniQure. Conclusions: Intrastriatal/intrathalamic bilateral application of AAV5-CAG-miHtt(3e13/1e13gctotal) or PBS/Sucrose (54μl/216μl) similarly to AA5-CAG-GFP didn´t cause any neurological deficit in transgenic as well as wild type animals. The body weight increased in all animals 84 days after AAV5-CAG-miHtt/PBS-Sucroseintrastriatal/intrathalamic delivery with two exceptions TGanimal L616 (AAV5-CAG-miHtt1e 13g ctotal) and WTanimal L485 (AAV5-CAG-miHtt3e13gc total). The TG boar N176 from group VII (PBS/Sucrose) died during narcosis 56 days after IP/IT application), shortly after CSF and blood collection.The boar breathed heavily and intermittently, then the heart stopped beat and resuscitation failed. We immediately flushed them by 20 L of PBS with heparin and collect all tissues. All animals had normal food consumption. Inflammatory proces in AAV5-CAG-miHtt (1e13 gc total) seems to be similar to PBS/Sucrose injected animals accompanied with no detectable histological pathologies.
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Methodology of „acute“and transgenic Huntington disease model design in miniature pigs and its application for new methods of treatment and drugs testing in neurodegenerative diseases area
 Motlík, Jan ; Juhás, Štefan ; Juhásová, Jana ; Vodička, Petr ; Baxa, Monika
The aim of this methodology is to develop a biomedical model of Huntington's disease in miniature pigs and its use in practice for preclinical testing of new treatments both pharmacological and using new molecular biological methods.
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Methodology of spinal cord injury model design in miniature pigs and its application for new methods of treatment and drugs testing
 Maršala, M. ; Motlík, Jan ; Juhás, Štefan ; Juhásová, Jana
The goal of proposed methodology is to describe the possibilities of reproducible and adjustable minipigs spinal cord injury model utilization in preclinical practice for new methods of treatment and drugs testing potentially favorable for therapy of acute and chronic spinal cord injury in human. The methodology has been developed as a part of solution to program of applied research and experimental development ALFA Technology Agency of the Czech Republic, subprogram Progressive technologies, materials and systems (TA01011466; 80%) and Operational Program Research and Development for Innovation Ministry of Education, Youth and Sports (project ExAM - CZ.1.05./2.1.00/03.0124; 20%).
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National Repository of Grey Literature : 13 records found   previous11 - 13  jump to record:
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