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Ultrastructural interactions of larval bird schistosome Trichobilharzia regenti and immune cells of hosts nervous system
Krčmářová, Veronika ; Bulantová, Jana (advisor) ; Hodová, Iveta (referee)
Trichobilharzia regenti is a neurotropic fluke belonging to family Schistosomatidae. Larvae called schistosomula migrate in the definitive hosts (anseriform birds) throuth the central nervous system (CNS) to their final location in nasal mucosa, where they mature and lay eggs. In contrast with that, the infection of accidental mammalian hosts (including human), is often stopped already in the skin immediately after entering the host. However, some schistosomula are able to reach CNS of experimentally infected mice, and survive there temporarily. Reaction to the CNS infection of mice is usually provided by microglia, astrocytes or the other immune cells infiltrated from the hosts blood. Parasite protects itself against the host reaction with its tegument. It does not serve only as mechanical barrier, but also as main secretoric organ that is capable of active immune evasion. Changes within CNS of the vertebrate hosts, caused by migrating schistosomula of T. regenti, were already described by routine histological and immunohistochemical methods. Till now, there was a lack of informations about interactions of immune cells of the host and the tegument of the parasite on ultrastructural level. To fill this gap in knowledge, two different methods were used: (1) imunohistochemistry in light and electron...
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Morphology of Fascioloides magna life stages and histopathological changes in selected species of definitive hosts
Košťáková, Monika ; Kašný, Martin (advisor) ; Hodová, Iveta (referee)
Fascioloides magna is a trematode originating in North America. It is a significant pathogen, especially in cervids, but it can also infect ruminants kept for agricultural purposes.Adult trematodes are found in the liver tissue of their definitive hosts, where they can survive for a long period of time and produce large quantity of eggs. Those are passed through bile ducts and intestine with feces into the external environment. This thesis is focused on the morphology of individual developmental stages of F. magna. To this date only very limited number of literary resources concerning the topic had been published. Recorded results are compared with literary resources on Fasciola hepatica, a closely related trematode. The morphological characteristics of the individual developmental stages were studied by using histological, electron-miscroscopical and other methods (fluorescent labelling). The thesis also describes pathological changes of the definitive hosts' liver tissue. By means of the stated methods, the thesis expands the existing knowledge on the morphology of F. magna especially of surface structures and the distribution of the senzoric organs, some of which have been described for the very first time. Powered by TCPDF (www.tcpdf.org)
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