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Vlastnosti a regulace objemově-závislých aniontových kanálů u astrocytů.
Harantová, Lenka ; Anděrová, Miroslava (advisor) ; Moravec, Jan (referee)
Animal cells need to preserve constant volume in the face of osmolarity perturbations to function properly. To regain their original volume after hyposmotically induced swelling, most cell types extrude intracellular electrolytes and organic osmolytes accompanied by osmotically driven water. This process is termed regulatory volume decrease and is ensured by various ion channels and transporters. Recently, much attention has been focused on the ubiquitous volume-regulated anion channels activated by cell swelling. VRACs are moderately outwardly rectifying with intermediary conductance, permeable to inorganic anions and organic osmolytes and sensitive to broad-spectrum anion channels blockers. Functional properties of VRACs in astrocytes are particularly interesting, because many brain pathologies, such as ischemia, traumatic brain injury or hyponatremia, are associated with marked astrocytic swelling and VRACs could thus constitute a possible target for therapy of cerebral edema. Furthermore, VRACs are thought to play a role in cell cycle progression, cell migration, apoptosis and intercellular communication. Despite intensive research, VRACs molecular identity and mechanism of their activation and regulation are still unclear. This work summarizes known facts about VRACs, accentuating their...
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Final report about research in Sokolov post mining sites in 2019 based on agreement between ENKI ops and Institute of Soil Biology BC CAS
Frouz, Jan ; Bartuška, Martin ; Kopecký, T. ; Kučera, J. ; Bednářová, E. ; Krůček, M. ; Král, K. ; Kotápišová, M. ; Moradi, J. ; Lin, Qiang ; Dini-Andreote, F. ; Meador, Travis Blake ; Angel, Roey ; Harantová, L. ; Heděnec, P. ; Li, Lingjuan ; Baldrian, P. ; Angst, Šárka
The report brings main results of the research about soil and ecosystem recovery in post mining landscape near Sokolov based on agreement between ENKI ops and Institute of soil biology, BC CAS.
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Volume-regulated anion channels in astrocytes- in vitro and in situ analysis
Harantová, Lenka ; Anděrová, Miroslava (advisor) ; Vargová, Lýdia (referee)
Astrocytes need to preserve constant volume in the face of osmolarity perturbations to function properly. To regain their original volume after hyposmotically induced swelling, they extrude intracellular electrolytes and organic osmolytes, such as inorganic ions, excitative amino acids or polyols, accompanied by osmotically driven water. This process is termed regulatory volume decrease and is ensured by various ion channels and transporters. Recently, much attention has been focused on the ubiquitous volume-regulated anion channels activated by cell swelling. VRACs are moderately outwardly rectifying with intermediary conductance, permeable to inorganic anions and organic osmolytes and sensitive to broad-spectrum anion channels blockers. Using patch-clamp technique we aimed to characterize VRACs in cultured cortical astrocytes isolated from neonatal Wistar rats and to elucidate the effect of intracellular Na+ on VRAC activity. In addition, we also intended to characterize these channels in situ in brain slices of 10 - 12 days old rats, focusing mainly on hippocampal astrocytes. To induce astrocytic swelling, we exposed astrocytes to hypotonic solution (250 mOsm). In agreement with previous findings, we showed that cultured cortical astrocytes activate VRAC currents upon exposure to hypotonic stress, which...
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Vlastnosti a regulace objemově-závislých aniontových kanálů u astrocytů.
Harantová, Lenka ; Moravec, Jan (referee) ; Anděrová, Miroslava (advisor)
Animal cells need to preserve constant volume in the face of osmolarity perturbations to function properly. To regain their original volume after hyposmotically induced swelling, most cell types extrude intracellular electrolytes and organic osmolytes accompanied by osmotically driven water. This process is termed regulatory volume decrease and is ensured by various ion channels and transporters. Recently, much attention has been focused on the ubiquitous volume-regulated anion channels activated by cell swelling. VRACs are moderately outwardly rectifying with intermediary conductance, permeable to inorganic anions and organic osmolytes and sensitive to broad-spectrum anion channels blockers. Functional properties of VRACs in astrocytes are particularly interesting, because many brain pathologies, such as ischemia, traumatic brain injury or hyponatremia, are associated with marked astrocytic swelling and VRACs could thus constitute a possible target for therapy of cerebral edema. Furthermore, VRACs are thought to play a role in cell cycle progression, cell migration, apoptosis and intercellular communication. Despite intensive research, VRACs molecular identity and mechanism of their activation and regulation are still unclear. This work summarizes known facts about VRACs, accentuating their...
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E-customs´ systems and projects and their implementation in the Czech Customs administration
Harantová, Lenka ; Novák, Radek (advisor) ; Uhlíř, Karel (referee)
Diploma work called "E-customs'systems and projects and their implementation in the Customs administration of the Czech Republic" is intended to provide interested parties with an overview of systems, projects and key issues related to the evolution of the European electronic customs initiative and the present state of play especially in the Czech Customs administration. Subject of e-Customs is a collection of information customs systems and projects in order to exchange data and communicate between the customs and economic operators in a secure manner. Systems and project of e-Customs are set up in the individual parts of the present work with the stress of the link to legal, operational and technological instruments, both at Community and national level to allow for an efficient implementation of electronic customs systems. The work includes evaluation of the successfully launched and today stable transit and export systems and recommendation to accept measures leading to higher efficiency from the side of the Czech customs administration. The work is a sufficient information source of the e-Customs programme devoted not only to employees of the Czech Customs administration, but to all subjects related to customs procedures.
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