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Optimization of conditions for qualitative and quantitative analysis of farnesol and tyrosol using UPLC-MS/MS.
Greguš, Petr ; Nováková, Lucie (advisor) ; Šatínský, Dalibor (referee)
APLICATION OF UHPLC/MS/MS TECHNIQUE FOR QUALITATIVE AND QUANTITATIVE ANALYSIS OF FARNESOL AND TYROSOL, AS A PRODUCT OF METABOLISM OF CANDIDA ALBICANS Petr Gregus 1 , Lucie Novakova 1 1 Charles University in Prague, Faculty of Pharmacy in Hradec Kralove, Heyrovskeho 1203, 50005 Hradec Králové SUMMARY : Candida albicans is generally one of the most often isolated fungal pathogens in human body. It is a common cause of nosocomial infections, bloodstream infections, urinary infections and infections of digestive tract. One of the most infectious diseases is vaginal candidosis, which affects most of healthy population of women. Candida albicans can grow as hyphae, pseudohyphae, or budding yeast. The availability of this diversity - the variability of the internal morphology is of great benefit for the oraganism's lifestyle. Morphological change of yeast forms to pseudomycelium or mycelium (hyphae) characterizes the transition from colonial statute to invasive. In addition to the physical-chemical factors during these transformation procedures - farnesol and tyrosol participate as well as quorum sensing compounds. Farnesol which, if accumulated over a certain limit, it prevents the conversion of yeast to the mycelium. Tyrosol, which accelerates the conversion in to the form of the germ (germ tubes), namely the...
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Optimization of conditions for qualitative and quantitative analysis of farnesol and tyrosol using UPLC-MS/MS.
Greguš, Petr ; Nováková, Lucie (advisor) ; Šatínský, Dalibor (referee)
Direct activation of C-H bond in tetrazoles Richard Lubojacký, 5th grade Supervisor: Prof. RNDr. Milan Pour, Ph.D. Department of Inorganic and Organic Chemistry, Faculty of Pharmacy in Hradec Králové Within the framework of this diploma thesis, direct arylation and alkenylation of 1- substituted tetrazoles at C5 was developed and optimized. An economical preparation of a range of 1,5-disubstituted tetrazoles was thus made possible. In these reactions, Pd-catalysis in the presence of CuI and Cs2CO3 was found as optimal. Contrary to similar reactions of imidazoles and purines, it was necessary to incorporate phosphine ligands in order to stabilize Pd - intermediates and prevent them from a premature decomposition into cyanamides. A library of 1,5-disubstituted tetrazoles with very promising yields has been prepared using this method.
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Optimization of conditions for qualitative and quantitative analysis of farnesol and tyrosol using UPLC-MS/MS.
Greguš, Petr ; Nováková, Lucie (advisor) ; Šatínský, Dalibor (referee)
Direct activation of C-H bond in tetrazoles Richard Lubojacký, 5th grade Supervisor: Prof. RNDr. Milan Pour, Ph.D. Department of Inorganic and Organic Chemistry, Faculty of Pharmacy in Hradec Králové Within the framework of this diploma thesis, direct arylation and alkenylation of 1- substituted tetrazoles at C5 was developed and optimized. An economical preparation of a range of 1,5-disubstituted tetrazoles was thus made possible. In these reactions, Pd-catalysis in the presence of CuI and Cs2CO3 was found as optimal. Contrary to similar reactions of imidazoles and purines, it was necessary to incorporate phosphine ligands in order to stabilize Pd - intermediates and prevent them from a premature decomposition into cyanamides. A library of 1,5-disubstituted tetrazoles with very promising yields has been prepared using this method.
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Optimization of conditions for qualitative and quantitative analysis of farnesol and tyrosol using UPLC-MS/MS.
Greguš, Petr ; Šatínský, Dalibor (referee) ; Nováková, Lucie (advisor)
APLICATION OF UHPLC/MS/MS TECHNIQUE FOR QUALITATIVE AND QUANTITATIVE ANALYSIS OF FARNESOL AND TYROSOL, AS A PRODUCT OF METABOLISM OF CANDIDA ALBICANS Petr Gregus 1 , Lucie Novakova 1 1 Charles University in Prague, Faculty of Pharmacy in Hradec Kralove, Heyrovskeho 1203, 50005 Hradec Králové SUMMARY : Candida albicans is generally one of the most often isolated fungal pathogens in human body. It is a common cause of nosocomial infections, bloodstream infections, urinary infections and infections of digestive tract. One of the most infectious diseases is vaginal candidosis, which affects most of healthy population of women. Candida albicans can grow as hyphae, pseudohyphae, or budding yeast. The availability of this diversity - the variability of the internal morphology is of great benefit for the oraganism's lifestyle. Morphological change of yeast forms to pseudomycelium or mycelium (hyphae) characterizes the transition from colonial statute to invasive. In addition to the physical-chemical factors during these transformation procedures - farnesol and tyrosol participate as well as quorum sensing compounds. Farnesol which, if accumulated over a certain limit, it prevents the conversion of yeast to the mycelium. Tyrosol, which accelerates the conversion in to the form of the germ (germ tubes), namely the...
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