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Changes in the content of gliadin protein fractions in two genotypes of winter wheat with different lengths of vegetation time depending on nitrogen fertilization with drought interaction.
Francová, Marie ; Zemanová, Jana (referee) ; Hrstka, Miroslav (advisor)
In this diploma thesis the influence of the nitrogen fertilization and drought on the change in the content of gliadin protein fractions in two genotypes of winter wheat Avenue and Tobac was studied. These two genotypes differ in vegetation time length. Half of the plants were fertilized using nitrogen fertilizer at 200 kg N/ha. One third of the plants were cultured under the conditions of early drought (in bloom season), other one third was grown under the conditions of of late drought (grain filling season), and last third was grown under the conditions of natural irrigation. Individual gliadin fractions were separated by using A-PAGE method and their content quantified by computer densitometry. Our results have shown increase in gliadin fractions content after nitrogen fertilization. Early drought itself caused significant increase in the levels of -gliadin fractions in Tobac genotype. Early and late drought in combination with nitrogen fertilization increased levels of gliadin fractions in Tobac genotype. Early drought in combination with nitrogen fertilization had no effect on Avenue genotype, except of -gliadin fractions which decreased significantly. Late drought in combination with nitrogen fertilization caused significant increase in gliadin content in Avenue genotype. The highest increase in gliadin content was observed in fraction -5 of the Tobac variety during interaction nitrogen fertilization with late drought.
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The Thought-world of utraquist priest Václav Rosa
Francová, Marie ; Zilynská, Blanka (advisor) ; Zdichynec, Jan (referee)
Václav Rosa was utraquist priest, who got his ordination in 1516. Since that time he had replaced several parishes in the Bohemia. He passed away in 1560. Besides casual experiences of lower clergy his notes contain the view into the contemporarythinkingand the perceptionof the world. This thesis follows up my bachelor thesis, which is called Václav Rosa's writings as source for cognitionof religious situationinthe first half of the 16thcentury. This master thesis is focused on Rosa's library and its content, his dreams and his opinions are compared with other utraquist authors, who are Šimon of Slaný, Jan Bechyňka, Vaněk Valečovský, Bohuslav Bílejovský, Jan Laetus and the Priest of Černovice. The aim of this thesis is to detail Rosa's personality and places him in the picture frame of utraquist clergy.
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Václav Rosa's writings as source for cognition of religious situation in the first half of the 16th century
Francová, Marie ; Zilynská, Blanka (advisor) ; Zdichynec, Jan (referee)
This thesis is based on Václav Rosa's writings, who was utraquist priest and lived on different parsonages between 1516 and 1560. Writings give information about his itinerary (places and period of his stay), circumstances each of utraquist parsonages with consideration to personal and property, permits pursue relationship between parish clergy and utraquist consistory and between parish clergy and local protectors. Moreover writings contain reflection of politics events, which happened in Prague and in nationwide level (1524, 1547). The analysis of major source was refilled of pursuing wider contexts with consideration to other sources, especially to Bartoš Písař's chronicle and to Sixt z Ottersdorfu's writings. The eleboration of thesis concentrated to capture inner circumstances in utraquist church, to Rosa's description as man and priest. This probe of individual life of one utraquist priest should serve as case study, which approximates life conditions of non-catholic clergy.
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Changes in the content of gliadin protein fractions in two genotypes of winter wheat with different lengths of vegetation time depending on nitrogen fertilization with drought interaction.
Francová, Marie ; Zemanová, Jana (referee) ; Hrstka, Miroslav (advisor)
In this diploma thesis the influence of the nitrogen fertilization and drought on the change in the content of gliadin protein fractions in two genotypes of winter wheat Avenue and Tobac was studied. These two genotypes differ in vegetation time length. Half of the plants were fertilized using nitrogen fertilizer at 200 kg N/ha. One third of the plants were cultured under the conditions of early drought (in bloom season), other one third was grown under the conditions of of late drought (grain filling season), and last third was grown under the conditions of natural irrigation. Individual gliadin fractions were separated by using A-PAGE method and their content quantified by computer densitometry. Our results have shown increase in gliadin fractions content after nitrogen fertilization. Early drought itself caused significant increase in the levels of -gliadin fractions in Tobac genotype. Early and late drought in combination with nitrogen fertilization increased levels of gliadin fractions in Tobac genotype. Early drought in combination with nitrogen fertilization had no effect on Avenue genotype, except of -gliadin fractions which decreased significantly. Late drought in combination with nitrogen fertilization caused significant increase in gliadin content in Avenue genotype. The highest increase in gliadin content was observed in fraction -5 of the Tobac variety during interaction nitrogen fertilization with late drought.
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