|
|
Optimization of optical tweezers for multimodal holographic microscope
Vaverka, Jan ; Antoš, Martin (referee) ; Dostál, Zbyněk (advisor)
This bachelor's thesis has described the principle of operation of interference microscopes with closer analysis of multimodal holograpfic microscope (MHM), which has been constructed at the Institut of Physical Engeneering (IPE) of Brno University of Technology. It has also analysed principles of optical trapping and has closely descirebed force effects on the trapped object with characteristic lenght bigger than wavelenght of trapping beam. It has designed optimization of existing optical tweezers module so that the module is connectable to the prototype of MHM, located at IPE. Simulation and optimization of the optical assembly of module have been performed, the mechanical design of module with the drawings has been designed. The module has been entered into production.
|
|
|
The tri-band fluorescent reader
Škrabalová, Denisa ; Čolláková, Jana (referee) ; Dostál, Zbyněk (advisor)
This bachelor thesis deals with the issue of laboratory testing and design of an optical instrument called a tri-band fluorescent reader, which is designed for fluorophores Alexa Fluor 488, Alexa Fluor 555 and Alexa Fluor 647. It contains a theoretical part with key knowledge not only in the natural sciences, such as physics, chemistry, biology, but also the current technical capabilities that are necessary for the practical part. In the practical part is drafting the objectives of the thesis, which are the measurement and evaluation of the samples examined using a monochromator, acquisition and evaluation of images using the existing system, build spectral analysis throughput, create a simulation of custom solutions and create a system engineering design. The mechanical model of the reader assembly was made after measuring the samples and the device and creating the simulation.
|
|
|
The analysis of limits for multimode fibre imaging
Štolzová, Hana ; Kozubek, Michal (referee) ; Dostál, Zbyněk (advisor)
Multimódová vlákna jsou zobrazovacím prostředkem s významným potenciálem v in-vivo mikroendoskopii. V poslední době tato metoda zaznamenala velký rozvoj, a to díky zdokonalování výpočetní a jiné techniky, například prostorové modulace světla. Cílem této práce bylo nalézt specifické limity zobrazování multimódovými vlákny a představit jejich počítačovou simulaci. Byl zkoumán vliv způsobu osvětlení optického systému obsahujícího multimódové vlákno na jeho schopnost fokusace a zobrazování. Analýzou dat získaných ze simulací a experimentu bylo zjištěno, že různá míra omezení Gaussovského svazku a plnění apertury multimódového vlákna má za následek významnou změnu zobrazovacích schopností systému. Při pozorování kvality fokusace bylo zjištěno, že nejlépe se projevují svazky málo omezené aperturou vlákna. Tento fakt byl potvrzen i experimentálním měřením. Zobrazování za použití svazků s podobnými hodnotami omezení (50%) projevovalo i nejlepší schopnost přenosu kontrastu. Avšak při analýze rozlišení dvou bodových objektů se jako nejvhodnější projevily svazky významně přeplňující numerickou aperturu vlákna, 100% a více. Přítomnost tohoto rozdílu poukazuje na skutečnost, že multimódové vlákno není zcela náhodné médium, ale propagace světla skrz multimódové vlákno projevuje znaky závislosti na vnějších zobrazovacích podmínkách, jako je například změna omezení osvětlovacího svazku. V této práci bylo představeno několik způsobů vyhodnocení kvality zobrazování pomocí multimódového vlákna. Každé z těchto kritérií podalo dílčí charakteristiku chování optického systému obsahujícího multimódové optické vlákno. Jednotlivé výsledky se neshodují na jednom konkrétním řešení a nutí osobu využívající zobrazovací systém obsahující multimódové vlákno ke zvážení několika aspektů, a to v jakém prostředí bude daný optický systém využívat a který parametr kvality zobrazení bude považovat za nejdůležitější.
|
|
|
Coherence Controlled Holographic Microscope with the digital optics
Vavřinová, Jana ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
The Digital Micromirror Device (DMD) technology has been developed especially for Digital Light Processing projectors, which allow the image projection. After this succesful implementation, and thanks to the commercial availibility and low initial cost of the DMD chip, a wide range of other applications became possible. Besides, it may be used in microscopy as a spatial light modulator. For example in Coherence-Controlled Holographic Microscope (CCHM) that finds its use especially for imaging and measurement of live-cell dynamic processes. The DMD chip placed in the illumination part of CCHM allows for broadening the application possibilities. Namely it could be different illumination mode experiments or tomographic applications. The master's thesis deals with the optical design of CCHM with digital optics, i. e. DMD chip. The selection of optical elements for CCHM, the experimental verification of the imaging setup and the process of designing the illumination part are described in detail. In the end, the analysis of different designs for illumination setup with the digital optics in object arm is carried out and the results are compared.
|
|
|
Confocal module for the Coherence Controlled Holographic Microscope
Kubátová, Eva ; Kozubek, Michal (referee) ; Dostál, Zbyněk (advisor)
The Coherence Controlled Holographic Microscope (CCHM) was developed at BUT Brno for a quantitative phase imaging of living cells. Nowadays it ocurres that its imaging properties are enhanced by the use of additional modules. In the present the microscope is equipped with the epifluorescence module, which allows observation of fluorescently marked living cells. This thesis is going to follow up on the development of this module and is going to extend its options by confocal imaging. The disadvantage of current multi-channel confocal microscopes is a mechanical rotation of the Nipkow discs, which causes undesired mechanical vibrations. That is why in this thesis it is replaced by Digital Micromirror Device. With its use was developed optical system of the whole confocal model, whose correct funcion was simulated in optical CAD. The experimentally verified prototype serves to test the imaging properties. On this basis is designed an application idea of the fluorescence confocal module, which will be possible to connect to the CCHM microscope.
|
|
|
System for measuring of pintrack dimensions
Vodička, Petr ; Dostál, Zbyněk (referee) ; Antoš, Martin (advisor)
In this bachelor thesis is processed a summary of contactless sensors for length measurement, that are commonly used in industry, it's physical principals and are described systems for measure dimensions of a steel construction. There is description of a steel carrier and classified ways the carrier can be deformed. A measuring gate for evaluation of status of steel carrier is designed and proper sensors are chosen and teste. Docomentation for measuring gate is created.
|
|
|
3D printed microscopes
Žáková, Veronika ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
Modular microscope building kits allow those interested in microscopy to get a hands-on introduction to the construction of a light microscope. For this reason, it would be appropriate to equip the laboratories of the Institute of Physical Engineering at Brno University of Technology with such a kit. The inspiration can be found in the open source project openUC2, which offers optical kits based on a combination of 3D printed and commercially available optical components. The aim of my work is to use the open source parts database and supplement it with my own designs that would enable the construction of specialized polarization and fluorescence microscopes. The first part of the thesis introduces light microscopy methods and the equipment that is essential for their proper operation. Next, the 3D printing methods that are applicable to the fabrication of the designed parts are summarized. Beyond the openUC2, the design of a Köhler illuminator, a motorized stage and a motorized objective slider based on commonly available parts are introduced. A transmission microscope, a reflective polarizing microscope and a light sheet microscope were designed based on the UC2 kit with my construction modules. The first two have been assembled and tested along with their control software.
|
|
|
The adjusting collimator for the Fluorescent holographic microscope
Hlaváčová, Kateřina ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
For the proper function of the Fluorescence olographic microscope, it is necessary to adjust all the optical components of the microscope. Furthermore, the precise adjustment is the very critical condition for proper imaging of the Coherence-controlled holographic microscope. Therefore, it is necessary to create a sight collimator for these microscopes for their adjustment. The fluorescence holographic microscope is based on an interference and holographic principles, whose history is mentioned in the theoretical part of the thesis. The existing state of the art of laser sight collimators and their use in practice is also mentioned. The optical and mechanical design of the laser sight collimator and its realization are described in the next part of the thesis. The software for detecting the black sight cross was created for the use of the laser sight collimator in practice. The software is necessary to evaluate the correctness of the alignment of the adjusted microscope. The descriptions of the adjustment procedures for the laser sight collimator and for the Fluorescence holographic microscope are mentioned in the last part of the thesis. These procedures are necessary for proper manipulation and use with the proposed laser sight collimator.
|
|
| |
|
|
Fiber guided illumination module
Kropáč, Vlastimil ; Kvasnica, Lukáš (referee) ; Dostál, Zbyněk (advisor)
This diploma thesis describes the design of the illumination system for a Coherence--Controlled Holographic Microscope (CCHM). The theoretical part mentions the history of microscopy, the principle of holography and individual types of interference microscopy. To get closer to the topic, individual light sources and an overview of current illumination systems are mentioned. The diploma thesis also describes the procedure of designing a fiber-optic illumination module for microscopy from optical design through design of construction to the last step, which is assembly and testing of the module.
|
guest ::
RSS feed.