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National Repository of Grey Literature 3 records found  Search took 0.02 seconds. 
The Evolution of Mitochondrial Processing Peptidase and Its Substrates.
 Chytrá, Dana ; Janata, Jiří (advisor) ; Vopálenský, Václav (referee)
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Characterization of gycin-rich loop of mitochondrial processing peptidase alpha subunit from Saccharomyces cerevisiae
 Chytrá, Dana ; Janata, Jiří (advisor) ; Zikánová, Blanka (referee)
Mitochondrial processing peptidase (MPP) is a heterodimeric enzyme which belongs to M16B subfamily of metaloendopeptidases. A universal function of this enzyme is in recognition and cleavage of great number of mitochondrial preprotein presequences, which differ in length and amino acid sequence. MPP consists of catalytical β-MPP and probably recognizing α-MPP. The most conservative region in α-MPP is GRL - glycine-rich loop. Its function is supposed in primary interaction with preprotein presequence. It is possible to study conformational change of GRL after binding the substrate by fluorescence experiments. In this diploma thesis the constructs coding the α-MPP with the single reporter tryptophan residue in the position 289 or 299 were prepared using site-directed mutagenesis. These forms of α-MPP were produced in E. coli BL21(DE3)+pGroESL. Activities of MPP dimer containing α-MPP with the single tryptophan residue in the reporter position were compared with MPP from wild type of S. cerevisiae. Used substrate was yeast malate dehydrogenase precursor with fused presequence (pMDH) from three organisms (yeast, mouse and melon). These presequences differ in their length. Activities of MPP dimer containing α-MPP with the single reporter tryptophan residue in the position 289 were about 70 % while...
Detailed record
The Evolution of Mitochondrial Processing Peptidase and Its Substrates.
 Chytrá, Dana ; Vopálenský, Václav (referee) ; Janata, Jiří (advisor)
Detailed record

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