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Nanofibrous scaffolds in controlled delivery of autologous growth factors
Buzgo, Matej ; Amler, Evžen (advisor) ; Gášková, Dana (referee)
Platelet preparations are a source of various autologous growth factors and have numerous applications in tissues engineering. The aim of this work was to development electrospun nanofiber scaffolds with platelet preparations. Scaffolds based on the adhesion of platelets on nanofiber meshes were developed. The scaffolds were able to enhance chondrocyte proliferation in vitro. The main disadvantage of this system is the burst release of growth factors immediately after adhesion. To overcome this, we developed coaxially electrospun scaffolds with incorporated alpha granules. Alpha granules are novel platelet preparations with high amounts of growth factors. This system was able to stimulate chondrocyte proliferation and maintain TGF- 1 concentrations for 7 days. Additionally, a novel drug delivery system with coaxially incorporated liposomes was developed. Liposomes incorporated into nanofibers remain intact and can be used for the delivery of various molecules. The ability to maintain HRP activity was compared to systems based on coaxial electrospinning with liposomes, coaxial electrospinning without liposomes and blend electrospinning. When compared to other systems, coaxial electrospinning with liposomes preserves enzyme activity twice as long. These results clearly indicate the potential of...
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Coaxial and emulsion nanofibers for biomedical use
Korbelová, Gabriela ; Buzgo, Matej (advisor) ; Pavlů, Barbora (referee)
The current treatment of tissue injuries deal with numerous problems, such as limited healing capacity and immune response of the body. In many cases such obstacles does not allow a full regeneration of the injured tissues and the full resumption of its original features. Controlled delivery of growth factors has considerable importance for inducing wound healing for a wide range of defects. Biocompatible and biodegradable nanofibrous carriers prepared by electrospinning represent a universal platform for the delivery of a wide range of substances in regulated manner. The goal of this thesis is to create an overview and assessment of the nanofibrous systems for controlled drug release created by electrospinning with a focus on growth factors delivery. Nanofiber carrier offers a number of advantages, they are able to consistently deliver bioactive substance into the site of injury, maintain bioactivity of the substance and stimulate the adhesion, proliferation and differentiation of target cells. Compared to the classic methods of tissue engineering using tissue grafts with the risk of their immune rejection these funkcionalized biodegradable carriers offers the possibility to create cell-free carrier systems. The work is divided into 4 chapters concerning the methods of preparation of nanofibrous...
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Coaxial and emulsion nanofibers for biomedical use
Korbelová, Gabriela ; Buzgo, Matej (advisor) ; Pavlů, Barbora (referee)
The current treatment of tissue injuries deal with numerous problems, such as limited healing capacity and immune response of the body. In many cases such obstacles does not allow a full regeneration of the injured tissues and the full resumption of its original features. Controlled delivery of growth factors has considerable importance for inducing wound healing for a wide range of defects. Biocompatible and biodegradable nanofibrous carriers prepared by electrospinning represent a universal platform for the delivery of a wide range of substances in regulated manner. The goal of this thesis is to create an overview and assessment of the nanofibrous systems for controlled drug release created by electrospinning with a focus on growth factors delivery. Nanofiber carrier offers a number of advantages, they are able to consistently deliver bioactive substance into the site of injury, maintain bioactivity of the substance and stimulate the adhesion, proliferation and differentiation of target cells. Compared to the classic methods of tissue engineering using tissue grafts with the risk of their immune rejection these funkcionalized biodegradable carriers offers the possibility to create cell-free carrier systems. The work is divided into 4 chapters concerning the methods of preparation of nanofibrous...
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Nanofibrous scaffolds in controlled delivery of autologous growth factors
Buzgo, Matej ; Amler, Evžen (advisor) ; Gášková, Dana (referee)
Platelet preparations are a source of various autologous growth factors and have numerous applications in tissues engineering. The aim of this work was to development electrospun nanofiber scaffolds with platelet preparations. Scaffolds based on the adhesion of platelets on nanofiber meshes were developed. The scaffolds were able to enhance chondrocyte proliferation in vitro. The main disadvantage of this system is the burst release of growth factors immediately after adhesion. To overcome this, we developed coaxially electrospun scaffolds with incorporated alpha granules. Alpha granules are novel platelet preparations with high amounts of growth factors. This system was able to stimulate chondrocyte proliferation and maintain TGF- 1 concentrations for 7 days. Additionally, a novel drug delivery system with coaxially incorporated liposomes was developed. Liposomes incorporated into nanofibers remain intact and can be used for the delivery of various molecules. The ability to maintain HRP activity was compared to systems based on coaxial electrospinning with liposomes, coaxial electrospinning without liposomes and blend electrospinning. When compared to other systems, coaxial electrospinning with liposomes preserves enzyme activity twice as long. These results clearly indicate the potential of...
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